Oxaliplatin (L-OHP, Jiangsu Hengrui, 50mg/pc), doxorubicin hydrochloride (ADM, Zhejiang Haizheng, 10mg/pc), 5-fluorouracil (5-FU, Jiangsu Nantong Jinghua Pharmacy, 0.25g/10ml), and VER (Verapamil, VER, Shanghai Hefeng Pharmacy, 5mg/2ml) were supplied by our hospital. The CCK-8 kit was purchased from Japan Dojindo Laboratories. The RNA extraction and reverse transcription kit were purchased from TIANGEN. 2×SYBR Green universal qPCR Master Mix was purchased from TIANGEN. Mouse-anti human CDKN2B/P15 and P-gP primary antibody were purchased from Abcam. The GADPH antibody was purchased from Sigma. The second antibody labeled by sheep-anti mouse HRP was purchased from Guizhou Jinqiao Biotechnology. The high-flux sequencing was performed by Guangzhou Ruibo. siRNA for gene transfection was purchased from Guizhou Ruibo. The overexpression plasmid and empty carrier TrueORF GOLD type were purchased from ORIGENE. The annexin V-PI double-staining kit was purchased from Beijing Beibo. The Lipofectamine 3000 was purchased from Invitrogen. Primer design and synthesis were performed by Shanghai Shanjing Biotechnology Company.
Sybr green universal qpcr master mix
Manufactured by Tiangen Biotech
The 2×SYBR Green universal qPCR Master Mix is a ready-to-use solution for quantitative real-time PCR (qPCR) applications. It contains SYBR Green I dye, DNA polymerase, reaction buffer, and optimized concentrations of MgCl2 and dNTPs.
Automatically generated - may contain errors
Lab products found in correlation
Rna extraction and reverse transcription kit, by Tiangen Biotech (2 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
P gp primary antibody, by Abcam (1 mentions)
Gadph antibody, by Merck Group (1 mentions)
Gapdh antibody, by Merck Group (1 mentions)
Annexin 5 pi double staining kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using sybr green universal qpcr master mix
1
Oxaliplatin-Doxorubicin-5FU Combination Therapy
2
Evaluation of Anti-cancer Drugs in vitro
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Through our hospital pharmacy, we obtained VER from Shanghai Hefeng Pharmaceutical Company at 5 mg/2 mL, oxaliplatin (L-OHP) from Jiangsu Hengrui at 50 mg/ampul, doxorubicin hydrochloride (ADM) from Zhejiang Haizheng at 10 mg/ampul, 5-FU from Jiangsu Nantong Jinghua Pharmaceutical Company at 0.25 g/10 mL. Cell Counting Kit 8 (CCK-8) was provided by Japanese colleagues at the Chemical Institute. The Tiangen Company provided RNA extraction and reverse transcription kit and 2×SYBR Green Universal qPCR Master Mix. Mouse anti-human UCHL1 primary antibody was obtained from the Abcam company (USA). GAPDH antibody was purchased from Sigma; goat anti-mouse HRP-labeled secondary antibody was obtained from Guizhou Jinqiao Biological Company; and high-throughput sequencing was commissioned by Guangzhou Ruibo company. The Guangzhou Ruibo Company also provided siRNA for gene transfection; empty vector, and overexpression plasmid was purchased from the Origene Company, TrueORF GOLD model. The Beijing Beibo Company provided Annexin V-PI double staining kit; Lipofectamine 3000 was obtained from Invitrogen Company; Shanghai Shanjing Biotechnology Company conducted primer design and synthesis.
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