The following Abs were used: mouse anti-GPR15 (MAB3654) and anti-GRK5 (MAB4539) from R&D Systems; rabbit anti-GPR15 (NBP1-02651) from Novus Biologicals; mouse anti-Myc (05-724) from EMD Millipore; rabbit anti-Myc (sc-789), rabbit anti-GRK6 (sc-566), rabbit anti–β-tubulin (sc-9104), rabbit anti-GRK2 (sc-562), rabbit anti-GRK6 (sc-566), and rabbit anti–14-3-3β (sc-629) from Santa Cruz Biotechnology; rabbit anti–phospho-PKA substrate (p-PKA sub, 9624), rabbit anti–phospho-PKC substrate (p-PKC sub, 2261), rabbit anti-Rab7 (9367), rabbit anti-LAMP1 (9091), rabbit anti-EEA1 (3288), rabbit anti-PKA C-α (5842), and rabbit anti–β-arrestin1/2 (D24H9) from Cell Signaling Technology; sheep anti-TGN46 (AHP500GT) from AbD Serotec; AF488-mouse anti-HA (A-21287), rabbit anti-AF488 (A-11094), Cy3-goat anti-mouse immunoglobulin G (IgG; A10521), Cy3-goat anti-rabbit IgG (A10520), AF488–donkey anti-sheep IgG (A11015), AF647–goat anti-rabbit IgG (A21245), and RPE–goat anti-mouse IgG (M30004-1) from Life Technologies; horseradish peroxidase (HRP)–goat anti-rabbit IgG (PI-1000) and HRP–goat anti-mouse IgG (PI-2000) from Vector Laboratory; AF488-Fab fragment of goat anti-mouse IgG (115-547-003) and normal goat-anti mouse IgG (115-005-146) from Jackson ImmunoResearch Laboratories; AF488-streptavidin (405235) from BioLegend; normal goat serum from Thermo Fisher; and human AB serum from Sigma-Aldrich.
Rabbit anti β arrestin1 2 d24h9
Manufactured by Cell Signaling Technology
Rabbit anti–β-arrestin1/2 (D24H9) is a primary antibody that specifically recognizes β-arrestin1 and β-arrestin2 proteins. It is used to detect and quantify these proteins in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Normal goat serum (ngs), by Thermo Fisher Scientific (1 mentions)
Sheep anti tgn46 ahp500gt, by Bio-Rad (1 mentions)
Human ab serum, by Merck Group (1 mentions)
Nitrocellulose membrane, by GE Healthcare (1 mentions)
Mouse anti α tubulin t5168, by Merck Group (1 mentions)
Rabbit anti snap tag, by New England Biolabs (1 mentions)
Rabbit anti β actin 4970, by Cell Signaling Technology (1 mentions)
2 protocols using rabbit anti β arrestin1 2 d24h9
1
Immunochemical Analysis of GPR15 and GRK
2
Immunoblotting Protocol for Protein Detection
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Samples were fractioned by SDS–PAGE on 8% gels under reducing conditions, immunoblotted onto nitrocellulose membranes (GE Healthcare) and bands detected by enhanced chemiluminescence (GE Healthcare) onto films developed on a Xograph Compact X5 processor.
Antibodies were primaries rabbit anti-β-arrestin-1/2 (D24H9, Cell Signaling, 1/1000), rabbit anti-SNAP tag (New England Biolabs, 1/500), mouse anti-α-tubulin (T5168, Sigma, 1/1000), rabbit anti-β-actin (4970, Cell Signaling, 1/1000), and mouse anti-GAPDH (6C5, Merck, 1/10,000); and IgG-HRP secondaries (Santa Cruz Biotechnology).
Antibodies were primaries rabbit anti-β-arrestin-1/2 (D24H9, Cell Signaling, 1/1000), rabbit anti-SNAP tag (New England Biolabs, 1/500), mouse anti-α-tubulin (T5168, Sigma, 1/1000), rabbit anti-β-actin (4970, Cell Signaling, 1/1000), and mouse anti-GAPDH (6C5, Merck, 1/10,000); and IgG-HRP secondaries (Santa Cruz Biotechnology).
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