All reagents and solvents were purchased commercially and used without further purification, unless specifically noted. N-Hydroxysuccinimide (NHS), 3,3′-dithiodipropionic acid, 1-hexadecanol, 4-dimethylaminopyridine (DMAP; 99%, J&K; Guangdong, People’s Republic of China), monomethoxy-poly(ethylene glycol) (mPEG; Mn =2,000 Da, Fluka; St Louis, MO, USA), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl; Shanghai Chemical Co., Shanghai, People’s Republic of China), N,N-dimethyl formamide (DMF), and Cur (Shanghai Yingxuan Co., Shanghai, People’s Republic of China). MCF-7, HEK293, and NIH/3T3 cells were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Fetal bovine serum (FBS) was purchased from Gibco (Life Technologies AG, Switzerland). Ultrapure Milli-Q water (MW 18.2) was used in all experiments.
N hydroxysuccinimide nhs
Manufactured by J&K Scientific
Sourced in China
N-hydroxysuccinimide (NHS) is a chemical compound that serves as an activating agent. It is commonly used in chemical reactions to facilitate the formation of stable amide bonds between carboxylic acid and amine groups.
Automatically generated - may contain errors
Lab products found in correlation
1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc, by J&K Scientific (3 mentions)
Dimethyl sulfoxide (dmso), by Sangon (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
4 dimethylaminopyridine dmap, by J&K Scientific (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Nih3t3, by American Type Culture Collection (1 mentions)
Sodium hydroxide (naoh), by J&K Scientific (1 mentions)
Milli q plus 185, by Merck Group (1 mentions)
Acetic anhydride, by Sinopharm (1 mentions)
Triethylamine, by Sinopharm (1 mentions)
Haucl4 4h2o, by Sinopharm (1 mentions)
Dulbecco s modified eagle s medium dmem ham s f12 medium, by Thermo Fisher Scientific (1 mentions)
Calcein am pi double stain kit, by Solarbio (1 mentions)
Cck 8 cell viability kit, by Solarbio (1 mentions)
Rat igg elisa kit, by Thermo Fisher Scientific (1 mentions)
Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Solarbio (1 mentions)
14 protocols using n hydroxysuccinimide nhs
1
Synthesis and Characterization of Curcumin-loaded PEG Conjugates
2
DOTA-Dendrimer-Hyaluronic Acid Nanoplatform
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Ethylenediamine core amine-terminated G5.NH2 poly(amidoamine) dendrimers with a polydispersity index (PDI) less than 1.08 were purchased from Dendritech (Midland, MI). DOTA-NHS was purchased from CheMatech (Dijon, France). HA with molecular weight of 6 k Da, sodium hydroxide, EDC, and N-hydroxysuccinimide (NHS) were purchased from J&K Chemical Ltd. (Shanghai, China). HCCLM3 cells (an HCC cell line) were obtained from the Liver Cancer Institute, Zhongshan Hospital, Fudan University (Shanghai, China). Fetal bovine serum (FBS), streptomycin, penicillin, and Dulbecco’s modified Eagle medium (DMEM) were obtained from Hangzhou Jinuo Biomedical Technology (Hangzhou, China). Triethylamine, acetic anhydride, HAuCl4·4H2O, and all other chemicals and solvents were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). The water used in all experiments was purified by a Milli-Q Plus 185 water purification system (Millipore, Bedford, MA) with a resistivity greater than 18 MΩ cm. Regenerated cellulose dialysis membranes (MWCO = 14,000 or 1,000) were acquired from Thermo Fisher Scientific (Pittsburgh, PA).
3
PFCA-Modified CS Nanoparticles for Anti-Cancer Therapy
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CS (molecular weight = 340 kDa, degree of deacetylation percent (DD%) ≥ 95%) was purchased from Aladdin Industrial Co. (Shanghai, China). PFCA was purchased from Fluorochem Limited Co. N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride crystalline (EDC) and N-hydroxysuccinimide (NHS) were provided by JK Chemical Co. (Beijing, China). Dimethyl sulfoxide (DMSO) was purchased from Sangon Biotech Co. (Shanghai, China). PBS was obtained from Beijing Solarbio Science Technology Co. Dulbecco’s modified Eagle’s medium (DMEM)/Ham’s F-12 medium, FBS, and penicillin/streptomycin were purchased from Life Technologies Co. (New York, USA). Anti-PDL1 (catalog no. BE0101) used for therapy was purchased from BIOCELL Biotech Co. Anti-VEGFA (catalog no. HPAB0330CQ), a kind of mouse anti-VEGFA recombinant antibody, used for therapy was purchased from Creative Biolabs Co. Eylea (catalog no. MB2806) used for therapy was purchased from Meilunbio Co. Rat IgG, FITC, and Cy5.5 were purchased from Sigma-Aldrich. The calcein-AM/PI double stain kit, bicinchoninic acid (BCA) protein assay kit, and CCK-8 cell viability kit were purchased from Beijing Solarbio Science Technology Co. The Rat IgG ELISA kit was purchased from Thermo Fisher Scientific.
4
Synthesis and Functionalization of Graphene-Based Nanomaterials
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Natural graphite flake (99.8% purity) was purchased from Sigma–Aldrich. Chloroauric acid (HAuCl4·3H2O, ≥49.0% Au basis), disodium hydrogen phosphate (Na2HPO4), sodium dihydrogen phosphate (NaH2PO4), phosphoric acid (H3PO4), sulfuric acid (H2SO4), HCl, NaBH4, potassium permanganate (KMnO4), BSA, ClCH2COONa, N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS) were purchased from J&K company (Beijing, China). Ethanol was purchased from Beijing Chemicals Co., Ltd. (Beijing, China). All the chemicals used are at least of analytical grade and directly used without additional purification. The water purified through a Millipore system (18.2 MΩ·cm) was used throughout.
5
Enzymatic Glucose Biosensor Fabrication
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Polyacrylamide solution (average Mw = 10,000, 50 wt % in H2O) and Nafion (product No. 274704) were purchased from Aldrich (Shang Hai, China). All aqueous solutions were prepared with deionized water (18 MΩ·cm−1) from a Millipore system. GOx and chitosan were purchased from Alfa Aesar (Shang Hai, China). . D-(+)-Glucose was bought from Sinopharm Chemical Reagent Co., Ltd. (Shang Hai, China). 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were obtained from J&K Scientific (Shang Hai, China). We used commercial compressed air from a cylinder to maintain the oxygen concentration during electrochemical experiments. All other reagents were analytical grade and used without further purification. All chemicals were stored in a 4 °C refrigerator until used.
6
Preparation of Amino-Modified FMT Nanoparticles
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FMT synthesis has been described in previous reports 45 (link), 46 . The procedures used to prepare the amino-modified FMT are as follows. FMT nanoparticles (100 mg, containing carboxyl groups on the surface) were first dissolved in a solution of 100 mL dimethyl sulfoxide containing 12 mg of dimethylaminopyridine (DMAP, 0.1 mmol) (Sigma-Aldrich, St. Louis, MO), and were then added to 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (J&K Scientific Ltd, Shanghai, China) (192 mg, 1 mmol), and N-hydroxysuccinimide (NHS) (J&K Scientific Ltd) (115 mg, 1 mmol). The resulting mixture was then stirred at room temperature for 2 h and 1, 3-diaminopropane (J&K Scientific Ltd) (150 mg, 2 mmol) was added to the mixture and stirred for another 24 h. Thereafter, the reaction mixture was added with excess acetone to obtain crude FMT-NH2 nanoparticles. The crude products were washed thrice with acetone and dried under reduced pressure.
7
Sodium Citrate-Alginate Adsorbent for Metal Ions
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Sodium citrate, sodium alginate (average molecular weight: 270,000, 200 ± 20 mPa·s), 2-morpholineethanesulfonic acid, N-hydroxysuccinimide (NHS), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC), ethylenediamine, and metal salts were all purchased from J & K Scientific Ltd (Beijing, China). Except that sodium alginate is chemically pure, all other reagents used in this study were of analytical grade. The experimental water used in this work was double-distilled water. Lake water was collected from Anqing Linghu Lake, and river water was obtained from the Anqing section of the Yangtze River.
The macro and micro morphological images of CA–SC were taken using a Canon digital camera and an electronic scanning electron microscope (SEM), respectively. The chemical composition and functional groups of the adsorbent were determined using an infrared spectrometer and X-ray photoelectron spectrometer (XPS). The concentration of each metal ion in a solution was measured using an inductively coupled plasma emission spectrometer (ICP-OES).
The macro and micro morphological images of CA–SC were taken using a Canon digital camera and an electronic scanning electron microscope (SEM), respectively. The chemical composition and functional groups of the adsorbent were determined using an infrared spectrometer and X-ray photoelectron spectrometer (XPS). The concentration of each metal ion in a solution was measured using an inductively coupled plasma emission spectrometer (ICP-OES).
8
Chitosan-Based Doxorubicin Delivery System
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Low molecular weight chitosan (Mn = 5000) and Doxorubicin hydrochloride (DOX) were obtained from Meilun Biotechnology Company (Dalian, China). Glutaric dialdehyde solution (25%), Fluorescein isothiocyanate (FITC), NIR-797-isothiocyanate and poly(2-hydroxyethyl methacrylate) (polyHEMA) were purchased from Sigma Chemical Co.. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC×HCl), 4-carboxyphenylboronic acid (CPBA), Ethylene diamine tetraacetic acid, N-hydroxysuccinimide (NHS) and 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenyl-formazan (MTT) were obtained from J&K. Human neuroblastom cancer cell line (SH-SY5Y), murine hepatic cancer cell line (H22) and human liver carcinoma cell line (HepG2) were obtained from KeyGEN BioTECH (Nanjing, China). Male ICR mice (18-22 g) were obtained from Nanjing University of Chinese Medicine (Nanjing, China).
9
Synthesis of Dopamine-PEG Conjugates
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Dopamine hydrochloride (DA) was purchased from Sigma-Aldrich Co. Pyrrole (PY), ethanol, and ammonia (28–32%) were obtained from Sinopharm Chemical Reagent Co., Ltd., (Shanghai, China), and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were purchased from J&K Scientific Ltd. (Beijing, China). Amine-PEG-Amine (NH2-PEG-NH2, 6 kDa, ≥99.0%) was purchased from Chemgen Pharma Co., Ltd. (West Bengal, India), and 2,2-biphenyl-1-picrylhydrazyl (DPPH) was purchased from Aladdin Co., Ltd (Shanghai, China). All reagents were used directly without further purification. MCF-7, Hela and HUVEC cell lines were purchased from the Wuhan Cell bank of Chinese Academy of Sciences.
10
Annexin V-FITC/PI Apoptosis Assay
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All reagents were commercially available. MNPs were synthesized by Materials Science and Engineering Laboratory at Southeast University (Nanjing, China). The manufacturer details of the reagents are listed as follows: EMO was purchased from Shanghai YuanYe Biotechnology Ltd (Shanghai, China); Cy7-NHS ester, N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were purchased from J&K Chemical Ltd (Shanghai, China). Dimethyl sulfoxide (DMSO), Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection agents and Matrigel, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sangon Biological Engineering Technology & Services Ltd (Shanghai, China). Bovine serum albumin (BSA) was purchased from Sigma-Aldrich (MO, USA); Human pancreatic cell line BxPC-3 was purchased from the Chinese Academy of Sciences Shanghai Branch Cell Bank (Shanghai, China); The hTERT-HPNE (Human Pancreatic Nestin Expressing) cell line was purchased from Shanghai BoQuaner Biological Science and Technology Ltd (Shanghai, China).
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