The following anti-human CD4 mAbs were used: BT-061 (Biotest AG, Dreieich, Germany), OKT-3 (BioLegend, San Diego, CA, USA), RPA-T4 (BD Pharmingen, San Jose, CA, USA), SK3 (BioLegend), MT310 (Santa Cruz Biotechnology, Dallas, TX, USA), QS4120 (Abnova, Neihu District, Taipei City, Taiwan), B-A1 (Acris Antibodies, San Diego, CA, USA), EDU-2 (Santa Cruz Biotechnology), MT441 (Ancell Corporation, Bayport, MN, USA), OKT-4 (BioLegend).
Mt310
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The MT310 is a versatile laboratory centrifuge designed for a variety of applications. It features a compact and durable construction, enabling efficient separation of samples. The MT310 is equipped with a brushless motor and digital speed control, ensuring precise and consistent performance.
Automatically generated - may contain errors
Lab products found in correlation
Rpa t4, by BD (1 mentions)
Ab237709, by Abcam (1 mentions)
Ab125212, by Abcam (1 mentions)
Ab7260, by Abcam (1 mentions)
Mab5564, by Merck Group (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Tissue tek oct compound, by Sakura Finetek (1 mentions)
Ab13970, by Abcam (1 mentions)
Sp8 confocal microscope, by Leica (1 mentions)
2 protocols using mt310
1
Characterization of Anti-CD4 Monoclonal Antibodies
2
Spinal Cord Tissue Preparation and Immunohistochemistry
Check if the same lab product or an alternative is used in the 5 most similar protocols
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After treatment for 10 and 60 days, the animals were anaesthetised and perfused with 4% paraformaldehyde. Subsequently, 4 cm‐long spinal cord samples were collected, immersed in 30% sucrose, and embedded in a Tissue‐Tek® O.C.T compound (Sakura Finetek, Torrance, CA, USA). Further, 15 μm sections were made using a Leica CM1950 cryostat (Leica Microsystems, Wetzlar, Germany). Immunohistochemistry was performed as described previously.28 , 29 Images were obtained using a Leica SP8 confocal microscope (Leica Microsystems).
The following primary antibodies were incubated overnight at 4°C: rabbit anti‐GFAP (ab7260, Abcam) 1:500, mouse anti‐Tuj‐1 (MAB5564, Millipore) 1:500, chicken anti‐GFP (ab13970, Abcam) 1:500, rabbit anti‐CD4 (MT310, Santa Cruz Biotechnology, Dallas, TX, USA) 1:500, rabbit anti‐CD8 (ab237709, Abcam) 1:500 and rabbit anti‐CD68 (ab125212, Abcam) 1:500. Fluorescent secondary antibodies from different host sources were labelled with Alexa Fluor 488, 568, or 647 (all 1:500, Invitrogen) and incubated with the samples for 1 h at 25°C.
The following primary antibodies were incubated overnight at 4°C: rabbit anti‐GFAP (ab7260, Abcam) 1:500, mouse anti‐Tuj‐1 (MAB5564, Millipore) 1:500, chicken anti‐GFP (ab13970, Abcam) 1:500, rabbit anti‐CD4 (MT310, Santa Cruz Biotechnology, Dallas, TX, USA) 1:500, rabbit anti‐CD8 (ab237709, Abcam) 1:500 and rabbit anti‐CD68 (ab125212, Abcam) 1:500. Fluorescent secondary antibodies from different host sources were labelled with Alexa Fluor 488, 568, or 647 (all 1:500, Invitrogen) and incubated with the samples for 1 h at 25°C.
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