Diabetes mellitus was induced in male C57BL/6 mice aging from 8 to 10 weeks old by a single intraperitoneal (i.p.) injection of STZ (100 mg/kg), which was dissolved in 100 mM citrate buffer (pH 4.5) as depicted previously [12 (link),13 (link)]. Control mice received an equivalent volume of citrate buffer. After one week, the mouse tail vein blood glucose levels were measured by the Roche Accu-Chek Active blood glucose monitor. The mice with a fasting blood glucose level more than 12 mmol/L were considered diabetic. All mice were allowed free access to standard laboratory chow and tap water for another 3 months. Eight weeks after STZ injection, the diabetic and control mice were randomly divided into two groups, which were received injection (i.p.) of normal saline or AS (10 mg/kg/day) for consecutive 4 weeks. In brief, four groups were included (1) vehicle-treated nondiabetic mice, (2) AS-treated nondiabetic mice, (3) vehicle-treated diabetic mice, and (4) AS-treated diabetic mice. At the end of the experiments, the mice were killed under anesthesia after cardiac function measurement. The body weight was recorded, and the blood samples, heart tissues were collected.
Accu chek active blood glucose monitor
Manufactured by Roche
Sourced in Germany, Switzerland
The Accu-Chek Active is a blood glucose monitor designed to measure and display blood glucose levels. It features a simple and intuitive user interface to facilitate blood glucose testing.
Automatically generated - may contain errors
4 protocols using accu chek active blood glucose monitor
1
Diabetic Mouse Model: STZ-Induced and AS Treatment
2
Plasma Metabolite and Insulin Resistance Analysis
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The levels of cholesterol, triglycerides, total proteins, albumin, and globulins in the plasma were measured with a commercially available kit (Bioclin, Belo Horizonte, MG, Brazil). The low-density lipoprotein cholesterol (LDL-c) and very-low-density lipoprotein cholesterol (VLDL-c) levels were calculated according to Friedewald et al. [44 (link)]. Blood samples were collected from the tail and glycemia was measured with an Accu-Chek Active blood glucose monitor (Roche, Mannheim, Germany) [42 (link)]. Insulin was determined using a rat/mouse insulin enzyme-linked immunosorbent assay (ELISA) kit from Millipore/Sigma-Aldrich (catalog number EZRMI13K-St Charles, MO, USA). The Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) was calculated using the following formula: HOMA-IR (mmol/L) = fasting glucose value (mg/dL) × fasting insulin value (ng/dL)/405 [45 (link)]. The β-cell function was obtained by the HOMA-β method using the following formula: HOMA-β = 20 × fasting insulin value (µU/mL)/fasting glucose value (mmoL/L) −3.5) [46 (link)].
3
Inducing Diabetes in C57BL/6 and PARP-1-/- Mice
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To induce diabetes, male C57BL/6 (WT) mice or PARP-1−/− mice (10 weeks old, Jackson Laboratories, ME, USA) were treated with STZ (sigma, 50mg/kg in citrate buffer, pH 4.5) by intraperitoneal injection for 5 consecutive days [26 (link)], while the control animals (male C57BL/6 mice) received the same volume of citrate buffer. Mouse tail vein blood glucose levels were monitored by analysis with the Roche Accu-Chek Active blood glucose monitor. The mice with a fasting blood glucose concentration >11.1 mmol/l were considered diabetic. Mice were housed in a pathogen-free animal care facility and allowed free access to food and water. PARP-1−/− mice were genotyped by PCR (Supplementary Figure 1 ).
4
Burn-Induced Metabolic Alterations
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The rats were fasted for 12 h before burn or sham injury. Eight animals were killed, and blood was drawn from the abdominal aorta. Fasting blood glucose levels were determined before injury (d0) and at days 1, 4, 7, and 14 after injury (fasted for 8 h before blood glucose measurement). Fasting blood glucose levels were determined using an Accu-chek Active Blood Glucose Monitor (Hoffmann-La Roche, Basel, Switzerland). Serum total testosterone was measured in duplicate using a radioimmunoassay kit (ICN-Biomedicals, Irvine, Calif). Serum and TA muscle cell lysate IGF-1 were assayed using commercially available immunoassay kits using the xMAP technology (Linco Research, St Charles, Mo).
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