Dmla full automatic microscope

The airway tissues were fixed with 4% paraformaldehyde for 24 h at room temperature. Then the tissues were washed overnight with tap water and dehydrated by gradient ethanol, which was prepared with different volume of 100% ethanol and double distilled water. Subsequently, the tissues were paraffin-embedded, sectioned, and dewaxed. After being stained with hematoxylin for 10 min, the tissues were further incubated with hydrochloric acid alcohol for 5 min. Then the tissues were further dyed with Masson solution (D026-1-2, Jiangcheng Bio, http://www.njjcbio.com/) for 5 min, 1% phosphomolybdic acid aqueous solution (G3472, Solarbio) for 3 min, and toluidine blue (T818873, Macklin) for 5 min at room temperature. After being soaked in gradient ethanol again for dehydration, the tissue slices were made transparent by rinsing in xylene. Finally, the tissue slices were sealed with neutral gum and the image of each section was collected under a DMLA full automatic microscope (Leica, Solms, Germany) at a magnification of 100 ×.

Lung and airway tissues were fixed with 4% paraformaldehyde (R008069, Rhawn) for 24 h at room temperature. Then the samples were washed overnight with tap water and dehydrated with gradient ethanol (E809057, Macklin, Shanghai, China) and double distilled water. Subsequently, the tissues were embedded into paraffin, sectioned, and dewaxed. Then the tissues or cells in BALF were stained by hematoxylin (B25380, Yuanye, Shanghai, China) for 10 min and further incubated with hydrochloric acid alcohol (AR0038, Boster, Wuhan, China, http://www.boster.com.cn/index/index.html) for 5 min. Then the samples rested in 50°C warm bath until the tissues appeared blue, and were further stained with eosin (C0109, Beyotime) for 1 min at room temperature. After soaking the samples in gradient ethanol again for dehydration, the sample slices were made transparent by soaking in xylene (R017750, Rhawn). Finally, the sample slices were sealed with neutral gum (N116470, Aladdin, Shanghai, China) and the image of each section was collected under a DMLA full automatic microscope (Leica, Solms, Germany) at a magnification of 100 ×.