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2 protocols using rpa32 s4 8

1

Comprehensive Antibody Panel for DNA Damage Response

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Primary antibodies used were from Cell Signalling unless otherwise mentioned: β-actin (Abcam #ab6276), cleaved PARP (#5625), CDK1 Y15 (#9111), CHK1 (#2360), CHK1 S345 (#2348), H2AX (#7631), H2AX S139 (Millipore #05-636), H3 (#9715), H3 S10 (#3377), RRM2 (ABNOVA #H00006241-M01), ATR (Santa Cruz #1887), ATR T1989 (Gene Tex #GTX128145), CHK2 (#2662), CHK2 T68 (Abcam #3501), KAP1 (Abcam #10484), KAP1 S824 (Abcam #133440), DNA-PKcs (Abcam #70250), DNA-PKcs S2056 (Abcam #18192), ATM (Abcam #78), ATM S1981 (Abcam #81292), RPA32 (Abcam #2175), RPA32 S4/8 (Bethyl Laboratories #A300-245A), RPA32 S33 (Bethyl Laboratories #A300-246A).
For secondary antibodies, Alexa 488 (#4408, #4412) and Alexa 647 (#4410, #4414) from Cell Signalling were used in immunostaining. IRDye800-conjugated (#925-32210, #926-33210) and IR680-conjugated (#926-68070, #926-68021) antibodies from LICOR were used in immunoblotting.
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2

Comprehensive Antibody Panel for DNA Damage

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The following antibodies were used in this study: histone H4 (Abcam, ab31830), ZNF280C (Abcam, ab83287), PCNA (Santa Cruz Biotechnology, Sc-56), p-Chk1 (S345) (Cell Signaling, 2348), GAPDH (Millipore), histone H3 (Abcam), RPA34 (Thermo Scientific, MA1-26418), MDC1 (Bethyl Laboratories, A300-051A), ATR (Bethyl Laboratories, A300-138A), CtIP (Abcam, ab155988), MRE11 (Genetex), RPA32 (S4/8) (Bethyl Laboratories, A300-245), phospho-histone H2A.X (Ser139) (20E3), HA (Covance, HA.11), and GFP (Invitrogen, A11122).
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