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Panc 1

Manufactured by Procell
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Sourced in China

PANC-1 is a cell line derived from a human pancreatic ductal adenocarcinoma. It is commonly used in research related to pancreatic cancer.

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Lab products found in correlation

Bxpc 3, by Procell (10 mentions) Aspc 1, by Procell (9 mentions) Sw1990, by Procell (9 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions) Cfpac 1, by Procell (7 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions) Miapaca 2, by Procell (4 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions) Hpde6 c7, by Procell (3 mentions) Hpde6, by Procell (3 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Lentivirus, by Hanheng Biotechnology (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Procell (2 mentions) Capan 2, by Procell (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Beyotime (2 mentions) Fetal bovine serum (fbs), by GE Healthcare (1 mentions) Capan 1, by American Type Culture Collection (1 mentions)

32 protocols using panc 1

1

Pancreatic Cancer Cell Lines and SLC1A5 Knockdown

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Two human pancreatic cancer cell lines (PANC-1 and SW1990) and a normal pancreatic duct epithelia cell line (HPDE6-C7) were purchased from Procell Life Science&Technology (Wuhan, China). PANC-1 cells were cultured in DMEM (Dulbecco’s Modified Eagle Medium) containing 10% FBS (fetal bovine serum) and 1% P/S (penicillin/streptomycin) (Procell, Wuhan, China). SW1990 cells were cultured in Leibovitz’s L-15 medium containing 10% FBS and 1% P/S (Procell, Wuhan, China). HPDE6-C7 cells were cultured in DMEM (Dulbecco’s Modified Eagle Medium) containing 10% FBS and 1% P/S (Procell, Wuhan, China). The culture conditions were 37°C with 5% CO2.
SLC1A5-specific shRNA and amplification plasmids were designed by Hanheng Biotechnology (Shanghai, China). Lentiviruses were also purchased from Hanheng Biotechnology (Shanghai, China) and were applied to transfect cells. The interference efficiency was tested by RT-qPCR after 72 h post-transfection.
Xu F., Wang H., Pei H., Zhang Z., Liu L., Tang L., Wang S, & Ren B.C. (2022). SLC1A5 Prefers to Play as an Accomplice Rather Than an Opponent in Pancreatic Adenocarcinoma. Frontiers in Cell and Developmental Biology, 10, 800925.
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2

Transfection of Pancreatic Cancer Cells

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Three human pancreatic cancer cell lines (AsPC-1, CFPAC-1, and PANC-1) were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). Human pancreatic normal cell lines (hTERT-HPNE) were obtained from Meisen Cell Biotechnology Co., Ltd. (Hangzhou, China). AsPC-1 and CFPAC-1 cells were maintained in Roswell Park Memorial Institute-1640 and Iscove’s Modified Dulbecco’s Medium (IMDM; Procell), respectively. PANC-1 and HPNE cells were cultivated in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; Procell). All media contained 10% fetal bovine serum (FBS; Procell) and 100 μg/mL penicillin-streptomycin solution (Procell). The humidity incubator was set to 37°C with 5% CO2.
Cells in six-well plates were transiently transfected with siRNA using Lipofectamine™ 3000 transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol. siRNAs were synthesized by GenePharma (Shanghai, China), including ALDH3B1 siRNA (sequence: 5′-GCU GAA GCC AUC GGA GAU UAG tt-3′), NCEH1 siRNA (sequence: 5′-CAA UGA UCG UUA ACA AUC Att-3′), and universal negative control (NC) siRNA.
Chen J., Liu Z., Wu Z., Li W, & Tan X. (2023). Identification of a chemoresistance-related prognostic gene signature by comprehensive analysis and experimental validation in pancreatic cancer. Frontiers in Oncology, 13, 1132424.
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3

Grape Seed Procyanidins Inhibit PANC-1 Cells

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The human PC cell line PANC-1 was obtained from Procell (http://www.procell.com.cn; Wuhan, China) and cultured in monolayers in Dulbecco's modified Eagle's medium (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (Hyclone; GE Healthcare Life Sciences, Logan, UT, USA) in a humidified incubator at 37°C and a 5% CO2 atmosphere. The GSP extract, obtained from JF-NATURAL (Tianjin, China; cat. no. J011003), contained monomeric (9.5%), dimeric (12.8%), trimeric (76.7%) and oligomeric (1%) procyanidins. The 100 µg GSPs extract was dissolved in 100 µl dimethylsulfoxide (DMSO; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) for 10 min at room temperature prior to addition to the cell culture media. The maximum concentration of DMSO in the media did not exceed 0.1%. PANC-1 cells were treated with 20 µg/ml GSP for 3, 12 and 24 h at 37°C. Additionally, cells were treated with DMSO for 3, 12 and 24 h at 37°C served as controls.
Wang W., Zhan L., Guo D., Xiang Y., Zhang Y., Tian M, & Han Z. (2018). Transcriptome analysis of pancreatic cancer cell response to treatment with grape seed proanthocyanidins. Oncology Letters, 17(2), 1741-1749.
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4

Genetically Engineered Cell Lines for Cancer Research

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SW1990, Panc-1, AsPC-1, and CFPac-1 were purchased from Procell Life Science & Technology Co. Ltd (Wuhan, China); BxPC-3, Capan-2, and HPDE6-C7 were purchased from BNBio Tech Co. Ltd; Capan-1 was purchased from ATCC. All cell lines were maintained as per the manufacturer’s propagation instructions. HPDE-KRASG12D cells were established by transfecting HPDE6-C7 cells with pCMV-KRASG12D plasmid (Miaoling Biology, Wuhan, China). CRISPR-Cas9 homology-directed repair (HDR) assay was carried out as previously described following Feng Zhang’s protocol [76 (link)] with sequence listed in Supplementary Information (SI) 21. The transfections were carried out using Lipofectamine 3000 (Thermo Fischer Scientific, Waltham, MA, USA).
Tan Y.Q., Sun B., Zhang X., Zhang S., Guo H., Basappa B., Zhu T., Sethi G., Lobie P.E, & Pandey V. (2024). Concurrent inhibition of pBADS99 synergistically improves MEK inhibitor efficacy in KRASG12D-mutant pancreatic ductal adenocarcinoma. Cell Death & Disease, 15(2), 173.
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5

Modulating Pancreatic Cancer Cell Signaling

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Two human pancreatic cancer cell lines (BxPC-3 and PANC1) and normal pancreatic duct epithelia cell line (HPDE6-C7) were purchased from Procell Life Science & Technology company (Wuhan, China). Three cells were all cultured in DMEM (Dulbecco’s Modified Eagle Medium) medium containing 10% FBS (Fetal bovine serum) and 1% P/S (Penicillin/ Streptomycin) (Procell, Wuhan, China). TLR3-specific shRNA and amplification plasmids were designed by HanHeng Biotechnology (Shanghai, China). Lentiviruses (HanHeng Biotechnology, Shanghai, China) were applied to transfect pancreatic cancer (PC) cells. The manipulation efficiency was tested by RT-qPCR after 72 h post-transfection.
Bai Z., Xu F., Feng X., Wu Y., Lv J., Shi Y, & Pei H. (2022). Pyroptosis regulators exert crucial functions in prognosis, progression and immune microenvironment of pancreatic adenocarcinoma: a bioinformatic and in vitro research. Bioengineered, 13(1), 1717-1735.
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6

Pancreatic Cancer Cell Line Cultivation

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Five human PC cell lines (ASPC-1, BxPC-3, SW1990, PANC-1 and Mia-PaCa-2) and human monocytic cells THP-1 were procured from Procell Life Science & Technology, Ltd (Wuhan, China). Human normal pancreatic ductal epithelial cell line (H6C7) was procured from Chuanqiu Biotech, Ltd (Shanghai, China). At the conditions of 37˚C with 5% CO2, all the above cells were cultured in RPMI-1640 medium (#11,875,085, Thermo Fisher Scientific, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; #10,100,147, Thermo Fisher Scientific).
ShRNA-LINC00460-1/-2 (sh-LINC00460-1/-2) and their negative control (sh-NC) were procured from Generalbiol, Ltd (Chuzhou, China). Overexpression-ANLN (pcDNA-ANLN), Overexpression-LINC00460 (pcDNA-LINC00460) and its negative control (pcDNA-NC), miR-503-5p mimics, miR-503-5p inhibitor and their negative control (miR-NC) were all procured from Ribo Biotech, Ltd (Guangzhou, China). The aforementioned agents were transfected into SW1990 and PANC-1 cells using a Lipofectamine RNAiMAX kit (#13,778,150, Invitrogen, Carlsbad, CA, USA). After treatment for 48 h, the transfected cells were harvested to perform the following trails. Additionally, THP-1 cells were utilized to incubate with PMA (100 ng/mL) for 24 h to reduce differentiation into macrophages.
Yao J., Gao R., Luo M., Li D., Guo L., Yu Z., Xiong F., Wei C., Wu B., Xu Z., Zhang D., Wang J, & Wang L. (2022). Exosomal LINC00460/miR-503-5p/ANLN positive feedback loop aggravates pancreatic cancer progression through regulating T cell–mediated cytotoxicity and PD-1 checkpoint. Cancer Cell International, 22, 390.
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7

PANC-1 Cell Culture Conditions

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Cell lines of PANC-1 was purchased from Procell Life Science (Wuhan, China) and cultured in the Dulbecco’s Modified Eagle Medium (DMEM; C11965500BT; Gibco) culture with 10% fetal bovine serum (FBS; cat no.2127186; VivaCell) and 1% penicillin and streptomycin (cat no.15140122; Gibco). The cell was maintained at 37℃ in a humidified 5% CO2 environment.
Zhu J., Shi Y., Lan S., Wang J., Jiang F., Tang C., Cai Y., Pan Z., Jian H., Fang H., Zhang Y, & Zhong F. (2023). Dissection of pyroptosis-related prognostic signature and CASP6-mediated regulation in pancreatic adenocarcinoma: new sights to clinical decision-making. Apoptosis, 28(5-6), 769-782.
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8

Culturing Human Pancreatic Cancer Cells

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The human pancreatic cancer cell lines PANC-1 and SW1990 were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). Cells were cultured in DMEM with 10% FBS (Biological Industries) and 1% penicillin–streptomycin (Beyotime Biotechnology, Shanghai, China) at 37°C in a humid atmosphere containing 5% CO2.
Xu Y., Lu L., Luo J., Wang L., Zhang Q., Cao J, & Jiao Y. (2021). Disulfiram Alone Functions as a Radiosensitizer for Pancreatic Cancer Both In Vitro and In Vivo. Frontiers in Oncology, 11, 683695.
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9

Culturing PANC-1 Human Pancreatic Cancer Cells

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The human pancreatic cancer cell line (PANC-1) was provided by Procell (Wuhan, China) and cultured in monolayers in Dulbecco's modified Eagle's medium (DMEM) (Invitrogen, Carlsbad, USA). All media were supplemented with 10% fetal bovine serum (Hyclone UT, USA) in the presence of 100 U/ml penicillin and 50 μg/m streptomycin (Beyotime, Shanghai, China) with humidified atmosphere of 5% CO2 and 95% air at 37 °C.
Zhang H., Wang X.W., Ye L., Li X., Sun R., & Tian J. (2021). Deciphering The Molecular Mechanism of Long Non-Coding RNA HIFA-AS1 Regulating Pancreatic Cancer Cells.
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10

PANC-1 Cell Response to Grape Seed Procyanidins

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The human PC cell line PANC-1 was obtained from Procell (Wuhan, China) (http://www.procell.com.cn/) and cultured in monolayers in Dulbecco's modified Eagle's medium (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (Hyclone; GE Healthcare Life Sciences, Logan, UT, USA) in a humidified incubator at 37°C and 5% CO2. The GSP extract, obtained from JF-NATURAL (Tianjin, China; catalog no. J011003), contained monomeric (9.5%), dimeric (12.8%), trimeric (76.7%) and oligomeric (1%) procyanidins. The 100 µg GSPs were dissolved in 100 µl dimethylsulfoxide (DMSO; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) for 10 min at room temperature prior to addition to cell culture media. The maximum concentration of DMSO in the media did not exceed 0.1%. PANC-1 cells were treated with 20 µg/ml GSP for 3, 12 and 24 h at 37°C, and the treated cells were used to prepare for SS3, SS12 and S24 samples, respectively. Additionally, control cell samples were treated with DMSO for 3, 12 and 24 h at 37°C, and then SC3, SC12 and SC24 samples were prepared accordingly.
Wang W., Zhan L., Guo D., Xiang Y., Tian M., Zhang Y., Wu H., Wei Y., Ma G, & Han Z. (2019). Grape seed proanthocyanidins inhibit proliferation of pancreatic cancer cells by modulating microRNA expression. Oncology Letters, 17(3), 2777-2787.
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