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Hif 1α

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HIF-1α is a protein subunit that plays a key role in the cellular response to hypoxia, or low oxygen conditions. It functions as the oxygen-sensitive component of the HIF-1 transcription factor complex, which regulates the expression of genes involved in adapting to hypoxic environments.

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Lab products found in correlation

4 hydroxynonenal hne, by Abcam (1 mentions) Vascular endothelial growth factor (vegf), by R&D Systems (1 mentions) Il 21, by RayBiotech (1 mentions) Human tnf α, by R&D Systems (1 mentions)

5 protocols using hif 1α

1

Quantification of Biomarkers in Samples

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Enzyme‐linked immunosorbent assay (ELISA) kits were used to measure the concentrations of hypoxia inducible factor (HIF)‐1α (Cell Biolabs, Inc), 5‐FU (anti‐5‐Fluorouracil antibody‐derived ELISA; MABEL Inc), ALT (BioVision, Inc) and amylase (MaxDiscovery amylase assay kit; Perkin Elmer) according to the manufacturers' instructions.
Sasaki T., Fujiwara‐Tani R., Kishi S., Mori S., Luo Y., Ohmori H., Kawahara I., Goto K., Nishiguchi Y., Mori T., Sho M., Kondo M, & Kuniyasu H. (2019). Targeting claudin‐4 enhances chemosensitivity of pancreatic ductal carcinomas. Cancer Medicine, 8(15), 6700-6708.
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2

Measuring HIF-1α, TFAM, and HNE

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ELISA kits were used to measure the concentration of HIF-1α (Cell Biolabs, Inc., San Diego, CA, USA), TFAM (MyBioSource, Inc., San Diego, CA, USA), and 4-hydroxynonenal (HNE) (Abcam, Cambridge, MA, USA). The assays were performed in accordance with the manufacturers’ instructions.
Takagi T., Fujiwara-Tani R., Mori S., Kishi S., Nishiguchi Y., Sasaki T., Ogata R., Ikemoto A., Sasaki R., Ohmori H., Luo Y., Bhawal U.K., Sho M, & Kuniyasu H. (2023). Lauric Acid Overcomes Hypoxia-Induced Gemcitabine Chemoresistance in Pancreatic Ductal Adenocarcinoma. International Journal of Molecular Sciences, 24(8), 7506.
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3

Quantifying HIF-1α and VEGF Levels

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HIF-1α (Cell Biolabs, Inc., San Diego, CA, USA) and vascular endothelial growth factor (VEGF; R&D systems, Minneapolis, MN, USA) levels were measured by ELISA according to the manufacturer’s instructions. The amounts of mouse HIF-1α, or VEGF present were determined by known concentrations, with a standard curve, respectively. Plates were read at 450 nm in a microplate reader.
Kang H.S., Kwon H.Y., Kim I.K., Ban W.H., Kim S.W., Kang H.H., Yeo C.D, & Lee S.H. (2020). Intermittent hypoxia exacerbates tumor progression in a mouse model of lung cancer. Scientific Reports, 10, 1854.
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4

Immunological Biomarkers in Lung Disease

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ELISAs of serum, BALF, and lung-tissue homogenates were conducted using commercial kits for IgE (Crystal Chem, Chicago, IL, United States), IL-6 and IL-17A (Anogen, Mississauga, Canada), IL-21, p-STAT3 (Tyr705), and total STAT3 (RayBiotech, Peachtree Corners, GA, United States), HDAC2 (EpiGentek, Farmingdale, NY, United States), and hypoxia inducible factor (HIF)-1α (Cell Biolabs, San Diego, CA, United States) according to the manufacturer’s instructions.
Xue Y., Zhou Y., Bao W., Fu Q., Hao H., Han L., Zhang X., Tian X, & Zhang M. (2021). STAT3 and IL-6 Contribute to Corticosteroid Resistance in an OVA and Ozone-induced Asthma Model with Neutrophil Infiltration. Frontiers in Molecular Biosciences, 8, 717962.
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5

ELISA Assay for Key Biomarkers

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Enzyme-linked immunosorbent assay (ELISA) kits were used to measure the concentrations of EGFR, phosphorylated EGFR, human TNFα (R&D Systems Inc., Minneapolis, MN, USA), CPE (TECHLAB, Blacksburg, VA, USA), HIF-1α (Cell Biolabs, Inc., San Diego, CA, USA), human CLDN4 (Cusabio Biotech, Wuhan, China), and 5-FU (anti-5-Fluorouracil antibody-derived ELISA, MABEL Inc., Kyoto, Japan), according to the manufacturers’ instructions.
Fujiwara-Tani R., Sasaki T., Luo Y., Goto K., Kawahara I., Nishiguchi Y., Kishi S., Mori S., Ohmori H., Kondoh M, & Kuniyasu H. (2018). Anti-claudin-4 extracellular domain antibody enhances the antitumoral effects of chemotherapeutic and antibody drugs in colorectal cancer. Oncotarget, 9(100), 37367-37378.
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