Cryovial

Manufactured by Greiner

Sourced in Germany

Cryovials are specialized containers designed for the storage and preservation of biological samples, such as cell cultures, tissues, or other materials, in ultra-low temperature environments, typically liquid nitrogen or mechanical freezers. They are constructed to maintain sample integrity and prevent contamination during long-term cryogenic storage.

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Lab products found in correlation

5 ml vacutainers, by BD (3 mentions) Cryogenic vials, by Greiner (1 mentions) Blood collection system, by BD (1 mentions) Conical tube, by Corning (1 mentions) Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) Pb 11 p10.1m, by Sartorius (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Sunrise tw, by Tecan (1 mentions) Trypletm, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Pentobarbital sodium, by Merck Group (1 mentions)

15 protocols using cryovial

Isolation and Preservation of Follicular Cells

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Follicular fluids from all follicles were collected individually in tubes (Nunc Centrifuge 11 ml, Nunc, Denmark). When an oocyte was obtained, the number of the follicle was recorded and the oocytes were numbered consecutively. Thus, each oocyte could be linked to an individual follicle and hence the correspondent MGC. Immediately after isolation of the COC, the MGC were isolated from the follicular fluid by centrifugation at 400 g in 8 min at room temperature. The pellet was re-suspended in 1 ml HEPES-buffered solution (Sydney IVF Follicle Flush Buffer, COOK Medical, Australia) and placed on a density gradient column (Histopaque 1077, Sigma-Aldirch, St-Louis, USA) and centrifuged at 400 g for 30 minutes. After centrifugation, the inter-phase containing the MGC was aspirated and the MGC washed twice by mixing with1 ml of the buffer solution followed by centrifugation at 400 g for 8 minutes at room temperature. After the second wash, pellet was re-suspended in 500 μl in a cryo-vial (CryoVial, 2 ml Greiner bio-one, Germany) and stored at -80°C until RNA extraction.
Within 1 hour after the oocyte retrieval a part of the cumulus mass was mechanically removed (18G needles) from the COC and transferred to a cryo-vial (CryoVial, 2 ml Greiner bio-one, Germany) with as little fluid as possible and stored at -80°C until RNA extraction.

Borup R., Thuesen L.L., Andersen C.Y., Nyboe-Andersen A., Ziebe S., Winther O, & Grøndahl M.L. (2016). Competence Classification of Cumulus and Granulosa Cell Transcriptome in Embryos Matched by Morphology and Female Age. PLoS ONE, 11(4), e0153562.

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Longitudinal Pig Biological Sample Collection

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Pig blood samples were collected at −7, 0, 3, 7, 10, 14, 17, 21, 28, 35, and 42 days post-inoculation (DPI) from the jugular vein or cranial vena cava using a blood collection system (Becton Dickinson, Franklin Lakes, NJ, USA) and vacutainer serum separation tubes (Kendall, Mansfield, MA, USA). Serum was harvested by centrifugation at 1500 × g for 5 min, aliquoted into 2 mL cryogenic vials (Cryovial^®, Greiner Bio-One, Monroe, NC, USA), and stored at −80 °C until testing.
Pen (2 pigs per pen) feces and oral fluids were collected every other day from DPI 0 to 42. Floor fecal samples were collected in 50 mL conical tubes (Corning^®, Corning, NY, USA) and aliquoted (~2 mg) in 2 mL cryogenic vials (Greiner Bio-One). Oral fluids were collected as previously described [31 (link),32 (link)]. Briefly, 3-strand 1.6 cm 100% cotton rope (Web Rigging Supply, Inc., Carrollton, GA, USA) was hung from a bracket fixed to one side of each pen for 30 min, giving pigs time to chew on and interact with the rope. The rope was then severed, placed in a plastic bag, passed through a clothes wringer (Dyna-Jet, Overland Park, KS, USA), and decanted into 50 mL conical tubes. Samples were aliquoted into 2 mL cryogenic tubes (Cryovial®), Greiner Bio-One) and stored at −80 °C until testing.

Yen L., Mora-Díaz J.C., Rauh R., Nelson W., Castillo G., Ye F., Zhang J., Baum D., Zimmerman J., Nelli R, & Giménez-Lirola L. (2022). Characterization of the Subclinical Infection of Porcine Deltacoronavirus in Grower Pigs under Experimental Conditions. Viruses, 14(10), 2144.

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Isolation of Intestinal Leukocytes from Mice

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Mice were perfused with 10 ml of PBS via intracardiac injection to remove circulating lymphocytes. Single cells were obtained from the spleen by pushing the organ through a 100-µm filter followed by RBC lysis. SI and LI tissue was sliced longitudinally, and digestive matter was expelled in cold PBS washes, and then the tissue was finely chopped with scissors in a 1.5-ml cryovial (Greiner Bio-One) in gut wash media (DMEM with 1% wt/vol FBS). Tissue was then incubated on a shaker at 37°C for 45 min in gut media (DMEM with 5% wt/vol FBS, 10 IU of penicillin and streptomycin) containing 500 mM dithiothreitol. Intraepithelial lymphocytes were isolated by pouring digested tissue through a 100-µm filter. Remaining tissue was incubated on a shaker at 37°C for 45 min in gut media containing 250 mg/ml collagenase D and 20 mg/ml DNase. Lamina propria lymphocytes were then isolated by pouring digested tissue through a 100-µm filter, and intraepithelial lymphocytes and lamina propria lymphocytes from their respective SI or LI compartment were combined. Cells were pelleted and resuspended in gut media with 40% Percoll PLUS, which was overlaid onto 80% Percoll PLUS, and cells were centrifuged at room temperature (RT) for 30 min (2,400 rpm, no brake; Sorvall ST40R centrifuge). Enriched viable intestinal leukocytes were then harvested from the buffy coat with a transfer pipette.

Macleod B.L., Elsaesser H.J., Snell L.M., Dickson R.J., Guo M., Hezaveh K., Xu W., Kothari A., McGaha T.L., Guidos C.J, & Brooks D.G. (2020). A network of immune and microbial modifications underlies viral persistence in the gastrointestinal tract. The Journal of Experimental Medicine, 217(12), e20191473.

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Plasma Methotrexate Pharmacokinetics

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Participants were screened and attended three visits at the CRF, Manchester, UK. Plasma samples were collected for the measurement of MTX concentrations from all patients prior to and following directly observed therapy of MTX at baseline (the reference standard). Samples were collected in K₂EDTA collection tubes at 0, 1, 2, 4, 8, 16, 24 hours and on 2 subsequent days within 7 days of observed MTX ingestion at a date/time convenient to the participant. Samples were placed on ice for a maximum of 30 min prior to sample preparation. The plasma fraction was prepared immediately by centrifugation at 1500g for 10 min at 4°C. Samples were divided into aliquots (0.5 mL) in cryovials (Greiner) and frozen by placing in a −80°C freezer. Samples were labelled with the patient ID, date and time of collection. Clinical information and reference standard results were available to the performers/readers of the index test.

Bluett J., Riba-Garcia I., Verstappen S.M., Wendling T., Ogungbenro K., Unwin R.D, & Barton A. (2019). Development and validation of a methotrexate adherence assay. Annals of the Rheumatic Diseases, 78(9), 1192-1197.

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Tissue Sampling and Cryopreservation

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The collected tissue samples were processed up to 4 hours from the collection. Tissues were transferred into a petri dish and were gently teased apart with a tissue forceps and then washed repeatedly with Phosphate Buffered Saline (PBS) to remove any blood. Healthy tissues that were free from blood were cut into smaller pieces (approximately 15mm in length) using a pair of tissue scissors. The processed tissues were then transferred into at least 3 separate Cryovials (Greiner, UK). These vials were snap frozen in -80 °C freezer.
Endometrium was transported on solid carbon dioxide (dry ice) inside a polystyrene box.

Manousopoulou A., Hamdan M., Fotopoulos M., Garay-Baquero D.J., Teng J., Garbis S.D, & Cheong Y. (2019). Integrated Eutopic Endometrium and Non-Depleted Serum Quantitative Proteomic Analysis Identifies Candidate Serological Markers of Endometriosis. Proteomics. Clinical applications, 13(3).

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Bioactive Glass Scaffold Characterization

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The BG-based scaffolds were stored in cryo-vials (Greiner Bio-One, Frickenhausen, Germany) containing 2.25 mL Dulbecco’s Modified Eagle Medium (DMEM), 4.5 g/L Glucose, 0.11 g/L Sodium Pyruvate, no L-Glutamine (all Thermo Fisher Scientific, Dreieich, Germany) under standard static cell-culture conditions (37 °C, 74% N₂, 21% O₂, and 5% CO₂). Prior to the initial transfer of the dry BG-scaffolds into the cryo-vial, DMEM was dropped onto the scaffolds to ensure the samples were well soaked in DMEM and to reduce artifacts in µCT analysis caused by parts within the scaffolds that could remain unfilled. During µCT-scanning, the DMEM was not removed from the vial, so the scaffolds were scanned in a liquid surrounding. Medium changes were performed twice a week. The BG-based scaffolds were immersed for eight weeks (56 days). Parallel to µCT-assessment, the medium was collected and frozen at −20 °C prior to pH measurement with a benchtop pH meter (PB-11-P10.1M; Sartorius, Göttingen, Germany).

Westhauser F., Ciraldo F., Balasubramanian P., Senger A.S., Schmidmaier G., Moghaddam A, & Boccaccini A.R. (2017). Micro-Computed-Tomography-Guided Analysis of In Vitro Structural Modifications in Two Types of 45S5 Bioactive Glass Based Scaffolds. Materials, 10(12), 1341.

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Quantification of Thyroid Hormone Levels

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Blood samples were obtained from the heart after sacrifice. The samples were
kept at 5°C until centrifugation at 1000×g. Serum was
distributed into sterile Cryo Vials (Greiner Labortechnik, Frickenhausen,
Germany) in volumes of 500 μL and immediately frozen at
−80°C until analysis. The levels of plasma thyroid hormones,
including triiodothyronine (T3), thyroxine (T4), and thyroid stimulating
hormone (TSH) were measured by enzyme-linked immunosorbent assay according
to the manufacturer’s protocol (Endocrine Technology, Old Bridge, NJ,
USA). The absorbance was measured 450 nm using a plate reader (Tecan Sunrise
TW, Salzburg, Austria).

Kim S.Y., Hong Y.P, & Yang Y.J. (2021). The Impairment of Thyroid Hormones Homeostasis after Short-Term Exposure to Di(2-ethylhexyl)phthalate in Adolescent Male Rats. Development & Reproduction, 25(4), 293-298.

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Anthropometry and Cardiovascular History

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Height (to the nearest cm) and weight (to the nearest 0.5 kg) were measured in participants wearing light clothing and no shoes [23] . Body mass index (BMI) was calculated as weight divided by the square of height in meters (kg/m 2 ). A self-administered questionnaire was used to collect a detailed history of previous cancer and arterial cardiovascular disease (CVD) events (i.e., stroke, angina pectoris, transient ischemic attack, and myocardial infarction).
Procedures for blood collection and storage of blood products have been previously described elsewhere [24, 25] . In brief, at baseline inclusion in 1994-1995 (Tromsø 4), nonfasting blood was collected from an antecubital vein into 5-mL vacutainers (Becton Dickinson, Le Pont de Claix, France) containing EDTA (K3-EDTA 40 µL, 0.37 mol/L per tube) as an anticoagulant. Platelet-poor plasma was prepared by centrifugation at 3000g for 10 minutes at room temperature, after which the supernatant was transferred into cryovials (Greiner Bio-One, Nürtingen, Germany) in 1-mL aliquots and stored at -80°C until further analysis.

Hansen E.S., Edvardsen M.S., Aukrust P., Ueland T., Hansen J.B., Brækkan S.K, & Morelli V.M. (2023). Combined effect of high factor VIII levels and high mean platelet volume on the risk of future incident venous thromboembolism. Journal of thrombosis and haemostasis : JTH, 21(10).

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Cryopreservation of hiPSC in Cryo Vials and Bags

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The effect of cryopreservation has been comparatively examined in two formats. Cryo vials (internal thread, 2 mL, Greiner bio-one) were used as standard cryo control, and 50 mL cryo bags (Miltenyi) were used for the bulk approach. For Cryo vials: hiPSC were dissociated into single cells with TrypLE^TM (Gibco) for approx. 3 min at 37 °C. TrypLE^TM was removed, and the cells were rinsed with culture medium, collected, and centrifuged at 500× g for 3 min. Then, 2 × 10⁷ cells were taken up in 1 mL cryomedium CryoStor^® CS10 (Stemcell Technologies) containing 10% DMSO. The solution was transferred into a 2 mL cryo vial and cooled at −1 °C/min from 4 °C to −80 °C and then stored in the nitrogen tank for at least 2 days (Cryotherm, Kirchen/Sieg, Germany). The storage time allowed a simulation of the handling procedure, and longer storage periods were not considered necessary as the cell characteristics and functionality were not expected to be impaired since metabolic reactions come to a halt at these temperatures (Yannas 1968, Shafa 2019). (For cryo bags: 1 × 10⁹ cells were dissociated as stated before, resuspended in 50 mL CryoStor^® CS10 and added to a 50 mL cryo bag (Miltenyi Biotec) using a syringe. A cooling rate of –1 °C/min from 4 °C to −80 °C was conducted using an ASKION workbench (C Line^®, WB220) and afterwards the cryo bag was stored in the nitrogen tank for at least 2 days.)

Meiser I., Alstrup M., Khalesi E., Stephan B., Speicher A.M., Majer J., Kwok C.K., Neubauer J.C., Hansson M, & Zimmermann H. (2023). Application-Oriented Bulk Cryopreservation of Human iPSCs in Cryo Bags Followed by Direct Inoculation in Scalable Suspension Bioreactors for Expansion and Neural Differentiation. Cells, 12(14), 1914.

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Cryopreservation and Thawing of Cells

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For freezing, trypsinated and detached cells (concentration no more than 5x10 6 cells/ml) were centrifuged at 800 rpm for 5 min in 15-ml falcon conical tubes (Greiner). The supernatant was discarded and the cell pellet resuspended in 1 ml of cold cryopreservation medium (90% v/v FBS and 10% v/v DMSO (Sigma-Aldrich, USA). The cell suspension was transferred to cryovials (Greiner) and maintained on ice for 30 min. The cryovials were then kept in a -20ºC freezer for 24h and transferred to a -80ºC freezer. For thawing, cryovials were removed from -80ºC freezer and immediately transferred to 37ºC. When they were completely thawed, aliquots were transferred into the 15-ml falcon tubes and cells pelleted at 800 rpm for 5 min.
The supernatant was discarded and pellet was resuspended in complete medium. Cells were maintained in defined growth conditions.

Abudabbus A., Badmus J.A., Shalaweh S., Bauer R, & Hiss D. (2017). Effects of Fucoidan and Chemotherapeutic Agent Combinations on Malignant and Non-malignant Breast Cell Lines. Current pharmaceutical biotechnology, 18(9).

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