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Cd3 bv605

Manufactured by Miltenyi Biotec
Sourced in Germany

The CD3-BV605 is a fluorochrome-conjugated antibody produced by Miltenyi Biotec. It is designed for the detection and analysis of CD3-positive cells using flow cytometry or other fluorescence-based applications.

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2 protocols using cd3 bv605

1

Rat Immune Cell Phenotyping

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The following rat reactive antibodies (clone, catalog number, dilution) from BioLegend, BD Biosciences, and Miltenyi Biotec were used: CD3-BV605 (1F4, 563949, 1:50), CD4-AF647 (OX-35, 203312, 1:100), CD4-PerCP-eFluor710 (OX-35, 46-0040-82, 1:200), CD44H-AF647 (OX-49, 203908, 1:100), CD8-BV786 (OX-8, 740913, 1:50), granzyme B-FITC (REA226, 130-118-341, 1:10), IFNγ-FITC (DB-1, 507804, 1:50), IFNγ-AF647 (DB-1, 507810, 1:50), and TNFα-PE (TNE-19.12, 559503, 1:50). Surface and intracellular staining was performed as described above. Events were collected on a BD LSR II flow cytometer following compensation with UltraComp eBeads (Invitrogen). Data were analyzed using FlowJo v7.6.5 (Tree Star).
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2

Multicolor Flow Cytometry Staining

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The antibodies CD3-BV605 (clone: OKT-3), CD4-PerCP (clone: OKT-4), CD8-PE (clone: RPA-T8) and CD69-PE (clone: FN50) were purchased from Miltenyi (Bergisch Gladbach, Germany). Ficoll was purchased from GE healthcare (Chicago, IL, USA), ultrapure water, DMSO, trifluoroacetic acid (TFA), Tris(2-carboxyethyl)phosphine (TCEP) was purchased form Sigma (MI, USA). All other reagents used were purchased from Thermo Fisher Scientific (MA, USA).
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