Cd3 bv605

Manufactured by Miltenyi Biotec

Sourced in Germany

The CD3-BV605 is a fluorochrome-conjugated antibody produced by Miltenyi Biotec. It is designed for the detection and analysis of CD3-positive cells using flow cytometry or other fluorescence-based applications.

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Lab products found in correlation

Ultracomp ebeads, by Thermo Fisher Scientific (1 mentions) Ultrapure water, by Merck Group (1 mentions) Cd4 percp, by Miltenyi Biotec (1 mentions) Cd8 pe, by Miltenyi Biotec (1 mentions) Cd69 pe, by Miltenyi Biotec (1 mentions) Ficoll, by GE Healthcare (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

2 protocols using cd3 bv605

Rat Immune Cell Phenotyping

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The following rat reactive antibodies (clone, catalog number, dilution) from BioLegend, BD Biosciences, and Miltenyi Biotec were used: CD3-BV605 (1F4, 563949, 1:50), CD4-AF647 (OX-35, 203312, 1:100), CD4-PerCP-eFluor710 (OX-35, 46-0040-82, 1:200), CD44H-AF647 (OX-49, 203908, 1:100), CD8-BV786 (OX-8, 740913, 1:50), granzyme B-FITC (REA226, 130-118-341, 1:10), IFNγ-FITC (DB-1, 507804, 1:50), IFNγ-AF647 (DB-1, 507810, 1:50), and TNFα-PE (TNE-19.12, 559503, 1:50). Surface and intracellular staining was performed as described above. Events were collected on a BD LSR II flow cytometer following compensation with UltraComp eBeads (Invitrogen). Data were analyzed using FlowJo v7.6.5 (Tree Star).

Hartlage A.S., Murthy S., Kumar A., Trivedi S., Dravid P., Sharma H., Walker C.M, & Kapoor A. (2019). Vaccination to prevent T cell subversion can protect against persistent hepacivirus infection. Nature Communications, 10, 1113.

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Multicolor Flow Cytometry Staining

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The antibodies CD3-BV605 (clone: OKT-3), CD4-PerCP (clone: OKT-4), CD8-PE (clone: RPA-T8) and CD69-PE (clone: FN50) were purchased from Miltenyi (Bergisch Gladbach, Germany). Ficoll was purchased from GE healthcare (Chicago, IL, USA), ultrapure water, DMSO, trifluoroacetic acid (TFA), Tris(2-carboxyethyl)phosphine (TCEP) was purchased form Sigma (MI, USA). All other reagents used were purchased from Thermo Fisher Scientific (MA, USA).

Meston D., Bi W., Rietschel T., Ham M.v., Leichert L.I., & Jänsch L. (2020). Identification of mtROS-sensitive processes in activated CD4⁺T cells.

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