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Inverted lsm 880

Manufactured by Zeiss
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The Inverted LSM 880 is a confocal laser scanning microscope designed for high-resolution imaging of live and fixed samples. It features a fully motorized and automated platform, enabling precise control of various imaging parameters. The system is equipped with a range of laser lines, detectors, and optical configurations to support a variety of imaging applications.

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Lab products found in correlation

Poly l lysine (pll), by Merck Group (1 mentions) Plan apochromat 63 1.4 na objective, by Zeiss (1 mentions)

Close spelling variants of this product

LSM 880 (3798 citations) LSM 880 inverted confocal microscope (54 citations) LSM 880 inverted microscope (25 citations) LSM 880 Airyscan inverted confocal microscope (8 citations) Zeiss LSM 880 Inverted laser scanning microscope (6 citations) LSM 880 Inverted Confocal Microscopy (6 citations) Zeiss LSM 880 inverted confocal laser scanning microscope (3 citations) Axio Observer Z1 Inverted LSM 880 NLO laser scanning confocal microscope (3 citations) 34-channel LSM 880 Fast Airyscan inverted microscope (3 citations) LSM 880 Airy inverted microscope (2 citations)

5 protocols using inverted lsm 880

1

Airyscan Imaging of Expanded Gels

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Expanded gels were placed into acrylic chambers with a square cut-out, attached to a no. 1.5 glass coverslip (Menzel Gläser), which had been coated with 0.1% (v/v) poly-l-lysine (Sigma-Aldrich) at room temperature for 30 min. Airyscan imaging was performed on an inverted LSM880 (Carl Zeiss, Jena), with a Plan-Apochromat ×63 1.4 NA objective with a working distance of 0.19 mm. AlexaFluor 488 and AlexaFluor 594 were excited with 488 and 561 nm DPSS lasers, while emission bands were selected using the in-built spectral detector.
Sheard T.M., Hurley M.E., Smith A.J., Colyer J., White E, & Jayasinghe I. (2022). Three-dimensional visualization of the cardiac ryanodine receptor clusters and the molecular-scale fraying of dyads. Philosophical Transactions of the Royal Society B: Biological Sciences, 377(1864), 20210316.
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2

Confocal Imaging of Brain Structures

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All male brains (Figure 2M, 2O, 2Q, 2S, and 2U) and all female subesophageal zones (Figure S4G) were imaged using a Zeiss Inverted LSM 880 laser scanning confocal microscope with a 25x/0.8 NA immersion-corrected objective. Glycerol was used as the immersion medium to most closely match the refractive index of the mounting medium Vectashield. Brains were imaged at 1024 X 1024 pixel resolution in X and Y with 0.5 μm Z-steps for a final voxel size of 0.2372 × 0.2372 × 0.5 μm3 (male brain) or 0.2076 × 0.2076 × 0.5 μm3 (female subesophageal zone). The laser intensity and gain were adjusted along the z axis to account for a loss of intensity due to depth and care was taken to avoid saturation and ensure that the deepest regions of the brain were visible. Confocal images of the brain were processed in ImageJ/FIJI (NIH) (Schindelin et al., 2012 (link)).
Herre M., Goldman O.V., Lu T.C., Caballero-Vidal G., Qi Y., Gilbert Z.N., Gong Z., Morita T., Rahiel S., Ghaninia M., Ignell R., Matthews B.J., Li H., Vosshall L.B, & Younger M.A. (2022). Non-canonical odor coding in the mosquito. Cell, 185(17), 3104-3123.e28.
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3

Confocal Imaging of Brain Structures

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All male brains (Figure 2M, 2O, 2Q, 2S, and 2U) and all female subesophageal zones (Figure S4G) were imaged using a Zeiss Inverted LSM 880 laser scanning confocal microscope with a 25x/0.8 NA immersion-corrected objective. Glycerol was used as the immersion medium to most closely match the refractive index of the mounting medium Vectashield. Brains were imaged at 1024 X 1024 pixel resolution in X and Y with 0.5 μm Z-steps for a final voxel size of 0.2372 × 0.2372 × 0.5 μm3 (male brain) or 0.2076 × 0.2076 × 0.5 μm3 (female subesophageal zone). The laser intensity and gain were adjusted along the z axis to account for a loss of intensity due to depth and care was taken to avoid saturation and ensure that the deepest regions of the brain were visible. Confocal images of the brain were processed in ImageJ/FIJI (NIH) (Schindelin et al., 2012 (link)).
Herre M., Goldman O.V., Lu T.C., Caballero-Vidal G., Qi Y., Gilbert Z.N., Gong Z., Morita T., Rahiel S., Ghaninia M., Ignell R., Matthews B.J., Li H., Vosshall L.B, & Younger M.A. (2022). Non-canonical odor coding in the mosquito. Cell, 185(17), 3104-3123.e28.
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4

Measuring Biomolecular Dynamics via FRAP

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FRAP measurements were carried out with a confocal laser scanning microscope (inverted LSM880, ZEISS) equipped with a 40× water objective (C-Apochromat, NA 1.2, ZEISS). mVenus was excited with an argon laser at 514 nm. For FRAP measurements, single condensates in or around the nucleus were chosen. Time series of 65 frames were acquired to observe fluorescence recovery. Acquisition times were approximately 300 ms per frame. Fluorescence recovery was analyzed individually for each bleached condensate by drawing narrow ROIs (63 (link)). For normalization, calculation of mobile fraction and half-life (τ½) a plugin written at the Stowers Institute for medical research (https://research.stowers.org/imagejplugins/index.html) was used in Fiji. Then, 9 to 10 individual puncta were photobleached from different cells, and used in each analysis. FRAP curves were generated from all data and mean curves with SD are shown. Experiments were performed at room temperature.
Hutin S., Kumita J.R., Strotmann V.I., Dolata A., Ling W.L., Louafi N., Popov A., Milhiet P.E., Blackledge M., Nanao M.H., Wigge P.A., Stahl Y., Costa L., Tully M.D, & Zubieta C. (2023). Phase separation and molecular ordering of the prion-like domain of the Arabidopsis thermosensory protein EARLY FLOWERING 3. Proceedings of the National Academy of Sciences of the United States of America, 120(28), e2304714120.
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5

Visualizing Sensory Neurons in Transgenic Flies

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To visualize sensory neuron cell bodies in ppk301-T2A-QF2, 15xQUAS-dTomato-T2A-GCaMP6 animals we dissected live sensory tissues using fine forceps, dipped in cold methanol for ~5 s, and mounted on a slide in glycerol. Appendages were viewed on a Zeiss Inverted LSM 880 laser scanning confocal with a 25x/0.8 NA immersion corrected objective at a resolution of 1024 × 1024 pixels and a voxel size of 0.2076 µm x 0.2076 µm x 1 µm.
Matthews B.J., Younger M.A, & Vosshall L.B. (2019). The ion channel ppk301 controls freshwater egg-laying in the mosquito Aedes aegypti. eLife, 8, e43963.
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