The CsPbBr3 QDs specimens were transferred to a high-vacuum sputter coater for protecting the layer deposition (JEE-4X, JEOL, Tokyo, Japan). Amorphous carbon layers with a thickness of 15–20 nm were coated on QDs specimens using pulsed carbon evaporation at 5 × 10−6 pa, where the sputtering current is above 50 A.
Jee 4x
Manufactured by JEOL
Sourced in Japan
The JEE-4X is a versatile laboratory equipment designed for various analytical applications. It features high-resolution imaging capabilities and advanced functionalities. The core function of the JEE-4X is to provide users with reliable and accurate data for their research and testing needs.
Automatically generated - may contain errors
Lab products found in correlation
Quantax 400, by Bruker (2 mentions)
Jfd 320, by JEOL (2 mentions)
Dsm 942, by Zeiss (1 mentions)
Jem 1010, by JEOL (1 mentions)
Polaron e5000, by Quorum Technologies (1 mentions)
Jsm 6510lv, by JEOL (1 mentions)
Ultra plus, by Zeiss (1 mentions)
Jsm 820, by JEOL (1 mentions)
Jsm 5200, by JEOL (1 mentions)
11 protocols using jee 4x
1
Protecting CsPbBr3 QDs with Carbon
2
SEM and EDS Analysis of SeNps
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Scanning electron microscopy (SEM) and Energy Dispersion Spectroscopy (EDS) of extracted SeNps were carried out using a JEOL JSM-6390LV scanning microscope equipped with an energy-dispersive X-ray (EDS) INCA micro-analytical system. Operating conditions were: accelerating voltage 20 kV, probe current 45 nA and counting time 60 s, with ZAF correction being provided on-line. The samples were coated with carbon, using a Jeol JEE-4X vacuum evaporator. Size distribution of the nanoparticles was determined by ImageJ analysis of multiple SEM images. Results presented are representative of three independent experiments.
3
Measurement of Inorganic Fibers in Lung Tissue
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We measured inorganic fibers such as asbestos fibers, non-asbestos mineral fibers, as described in our previous reports6 (link)). In brief, lung tissues were freeze-dried and ashed at low temperature by plasma asher (LTA-2S, Yanagimoto, Kyoto, Japan) for 6 hours. After ashing, 50 ml of distilled water was added and mixed with ultrasound shaker for 5 minutes. Then, filtered with nuclepore filter (pore size: 0.2 μm, Nuclepore Corp, Pleasanton, USA) by suction. The filter was coated with carbon by a suttering device (JEE-4X, JEOL, Akishima, Japan) and put on a nickel grid coated with carbon. Organic materials were washed away by chloroform The sample was put on a TEM grid and 5–50 squares of a grid were observed with a magnification of 10,000 times. We classified a dust with a length/diameter ratio of more than 3 as a fiber. The fibers were classified based on the analysis of the component of elements of the fibers by energy dispersive x-ray analyzer (EDXA, 7000Q, Kevex, Foster, USA). Minimum size of the length and diameter of the fibers were 0.2 μm and 0.02 μm respectively. DL was 0.04–0.22×106 fibers/g dry lung tissue. We classified asbestos by comparing samples with standard ones provided by the Japan Asbestos Association.
4
Geochemical Characterization of Planetary Samples
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Electron microprobe analyses of olivine, pyroxene, spinel, and matrix glass were performed using a JEOL JXA-8600M Superprobe with five wavelength-dispersive spectrometers at Instituto GEA, Universidad de Concepción, Chile. Analyzed samples were prepared by the conventional technique as epoxy blocks with mounted mineral grains. The polished surface of the samples was covered with a conducting carbon coating ~25 nm thick using a vacuum evaporator JEOL JEE-4X. All analyses were performed under 15 kV using Kα lines for all elements. Natural and synthetic minerals, simple oxides, and glasses were used as reference samples, and the set of these samples varied depending on the object of analysis. Those with USNM code were donated by the Smithsonian Institution, USA (ref. 58 (link)): diopside USNM 117733 (Si), USNM 137041 anorthite (Al), fayalite USNM 85276 (Fe), microcline USNM 143966 (K), chromite USNM 117075 (Cr), MnTiO3 (Ti and Mn), forsterite (Mg), jadeite (Na), wollastonite (Ca). Specific analytical conditions applied for different minerals and glass are described below. In all cases, corrections for the matrix effect were calculated by the ZAF method.
5
SEM Imaging and EDS Analysis of Powdered Samples
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The powdered DEP-samples were fixed to scanning electron microscopy (SEM) holder with the Quick Drying Silver Paint (Agar, UK) conductive glue and coated with thin layer of Au (about 10 nm) using a vacuum evaporator (JEE-4X, JEOL, Japan) to assure conductivity, protect the sample from heat destruction and to keep real parameters of the observed details. The samples were examined in a DSM 942 scanning electron microscope (Zeiss, Germany) in a secondary electron (SE) mode. Microscope parameters were set to high voltage (HV) = 10 kV and working distance (WD) = 6 mm. The elements present in investigated samples were determined using the energy dispersive X-ray spectrometry (EDS) using Quantax 400 (Bruker, Germany) system set to HV = 15 kV and WD = 20 mm.
6
Microscopic Analysis of Biological Samples
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Samples of A. salina nauplii—employed in the lethality test—and samples of rat erythrocytes—obtained from the hemolysis assay—were examined for morphological alterations induced by the aqueous extract of M. alcicornis. Some samples were dyed with starch blue and observed directly through light microscopy. For observations by SEM and TEM, samples were fixed in filtered sea water or isotonic saline solution containing 3 % glutaraldehyde and 0.1 M of sodium cacodylate, postfixed in 2 % OsO4 in cacodylate buffer, and dehydrated in an ethanol series. Then, for TEM analysis, samples were embedded in Epon epoxy resin and the blocks obtained were sliced (60 nm) in an ultramicrotome (Mtx RMC Boeckler Instruments, USA) and contrasted with uranyl acetate and lead citrate. These sections were observed in an electron microscope (JEM 1010, JEOL, USA) operated at 80 kV. For SEM analysis, the samples were dried in a critical-point dryer (Polaron E5000, Quorum Technologies, UK), covered with carbon in an evaporator (JEE4X, JEOL, USA) and with a thin sheet of gold in an ion sputterer (Polaron 11-HD, Quorum Technologies, UK), and finally observed with a scanning electron microscope (DMS 950, Zeiss International) at an accelerating voltage of 20 to 25 kV.
7
Preparation of S. aureus Cells for SEM Imaging
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For experiments on SEM, intact S. aureus 8325-4 cells or co-aggregates of LF3872 cells with S. aureus 8325-4 cells were preliminarily fixed in a 2.5% solution of glutaraldehyde in the cacodylate buffer overnight at 4 °C. Additionally, after three washes in same buffer, the cells were fixed in 1% solution of OsO4 in cacodylate buffer at 20 °C for 3 h. After fixation and subsequent washing in buffer, the cells were dehydrated in increasing concentrations of ethanol (from 30 to 100%) for 20 min at each stage. Next, the cells were placed in tert-butanol and left at 4 °C for 12 h. The studied samples were freeze-dried in JFD-320 (JEOL, Tokyo, Japan) and were placed on a disk and sputtered with a gold in a JEE-4X (JEOL, Tokyo, Japan) vacuum evaporator. Images were taken on the JSM-6510LV (JEOL, Tokyo, Japan) microscope.
8
Characterization of NaA Zeolite Morphology
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The size and morphology of NaA zeolites were investigated by the high-resolution scanning electron microscopy Carl Zeiss “ULTRA plus” (Ultra-High-Resolution Imaging, Jena, Germany). The powdered NaA zeolite samples were fixed to a scanning electron microscopy holder with the Quick Drying Silver Paint (Agar Scientific Ltd., Essex, UK) conductive glue and coated with a thin layer of carbon using a vacuum evaporator (JEE-4X, JEOL, Tokyo, Japan) to assure conductivity and protect the sample from heat destruction. The elements presented in investigated samples were determined using the energy dispersive X-ray spectrometry (EDS) using Quantax 400 (Bruker, Billerica, MA, USA) microanalysis system. The mean size of NaA particles was determined from HR-SEM micrographs by measuring the diameters of about 100 particles/per point in 6 synthesized products.
9
Textural Analysis of Materials via SEM
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Scanning electron microscope (SEM) imaging was employed to obtain a better understanding of the textural properties of the investigated materials. Images were made using SEM (JEOL JSM-820, Tokyo, Japan), and the accelerating voltage was 20 kV. To obtain better-quality images, all samples were covered with a gold layer using a JEOL JEE-4X vacuum evaporator.
10
Microstructure Analysis of Extrudate Samples
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The microstructure of extrudates was studied in a sample with 20% HW content. The sample was sliced into fragments that were then glued with silver paste onto specimen circles and sprayed with carbon and gold in a vacuum sprayer-type JEE 4X (JEOL, Tokyo, Japan). Microscopy analyses were carried out via an electron microscope of the type JSM 5200 (JEOL, Tokyo, Japan).
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