Bright field microscopy images (Figs. 2 a, 3 b) and videos of DNA particles were acquired with fully motorized and programmable Nikon Eclipse Ti-E inverted epifluorescence microscope, equipped with Perfect Focusing System (Nikon) and Grasshopper3 GS3-U3-23S6M camera (Point Gray Research), using a CFI Plan Apochromat λ 40 × /0.95 NA dry objective (Nikon). Temperature was controlled with the aforementioned copper-plate Peltier stage, and transmission imaging enabled by a small aperture. A transparent sapphire window (with large thermal conductance) was sandwiched between the sample and the copper plate to ensure a uniform temperature across the sample. Epifluorescence images of fluorescein-labeled particles (Fig. 3 b) were recorded with the same setup using blue LED illumination and a GFP filter cube (Semrock).
Gfp filter cube
Manufactured by IDEX Corporation
Sourced in United States
The GFP filter cube is a specialized optical component designed for fluorescence microscopy applications. It is used to selectively filter and isolate the excitation and emission wavelengths associated with green fluorescent protein (GFP) labeled samples. The filter cube consists of a set of precisely engineered optical filters that allow the passage of specific wavelengths of light, enabling the visualization and detection of GFP-labeled specimens.
Automatically generated - may contain errors
2 protocols using gfp filter cube
1
Epifluorescence Microscopy Protocol for DNA Particles
2
Calcium Flux Imaging of Differentiated iSNs
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Differentiated mature iSNs were loaded with Fluo‐4‐AM fluorescence dye (Invitrogen) by incubating at room temperature for 1 hour followed by 45 minutes period for de‐esterification. Cells were washed and incubated in Hanks' balanced salt solution, supplemented with 25 mM HEPES buffer and 5.5 mM glucose. Calcium flux was monitored using an Olympus IX81 inverted epi‐fluorescence microscope (Olympus, Markham, ON, Canada) coupled to a xenon arc lamp (EXFO, Quebec, QC, Canada). Indicated agonists, α,β‐meATP and capsaicin, were diluted in the aforementioned solution for a final stimulation concentration of 30 μM for α,β‐meATP and 1 μM for capsaicin. Fluorescence images were collected using an EMCCD (Electron Multiplying Charged Coupled Device) camera (Photometrics, Tucson, AR, USA) every 2 seconds through a GFP filter cube (Semrock, Rochester, NY, USA). Off‐line analysis of the intensity pattern of Fluo‐4 signal was performed in ImageJ (NIH, Bethesda, MD, USA).
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