The LC–MS/MS system consists of an Agilent LC 1100 series (Agilent Technologies, CA, USA) binary pump, a vacuum degeneration unit and an auto-sampler system connected to a 6490 triple quadrupole MS equipped with an Agilent jet stream technology electrospray ionization (ESI) source. Chromatographic separation was performed by an analysis Sepax BR-C18 (5 µm, 120 Å 1.0 × 100 mm) column. The maintaining temperature of column was set at 30 °C. The temperature of the auto-sampler was set at 4 °C. Of the sample solution 2 µL was injected and the analytes were eluted under the isocratic condition with acetonitrile and 0.1% formic acid in water (60%:40%, v/v) pumped at a constant flow of 0.10 mL/min. To detection of the resveratrol in the analytes, the MS/MS system was performed under negative ESI and the multiple reactions monitoring (MRM) mode. The MS operational parameters were: Argon as a collision gas; capillary voltage at 5 kV; gas temperature at 225 °C; gas flow 14.1 I/min; nebulizing gas at 40 psi; collision energies of 18 for resveratrol and the precursor to product ion transitions of 184.8–226.9 m/z for resveratrol.
Jet stream technology
Manufactured by Agilent Technologies
Sourced in United States
Jet Stream technology is a core component in select Agilent lab equipment. It utilizes high-speed gas flow to enhance sample introduction and separation processes. The technology aims to improve analytical performance and efficiency for laboratory applications.
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Lab products found in correlation
6460 triple quadrupole qqq mass spectrometer, by Agilent Technologies (2 mentions)
6460 qqq mass spectrometer, by Agilent Technologies (2 mentions)
Lc 1100 series, by Agilent Technologies (1 mentions)
6490 triple quadrupole ms, by Agilent Technologies (1 mentions)
Hypersil gold column, by Thermo Fisher Scientific (1 mentions)
1200 series hplc system, by Agilent Technologies (1 mentions)
6460 triple quadrupole mass spectrometer, by Agilent Technologies (1 mentions)
1290 infinity uhplc system, by Agilent Technologies (1 mentions)
Saccharin d4, by CDN Isotopes (1 mentions)
Agilent 1290 series uhplc system, by Agilent Technologies (1 mentions)
Zorbax rrhd eclipse plus c18, by Agilent Technologies (1 mentions)
Masshunter workstation qualitative analysis software, by Agilent Technologies (1 mentions)
Luna omega column, by Phenomenex (1 mentions)
Eclipse plus c18, by Agilent Technologies (1 mentions)
9 protocols using jet stream technology
1
Quantification of Resveratrol by LC-MS/MS
2
Quantitative Lipid Analysis of EVs
3
UHPLC-MS/MS Quantification of Compounds
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Quantification was performed
using an Agilent 1290 Infinity UHPLC system interfaced to a 6460 triple-quadrupole
mass spectrometer with ESI via Jet Stream technology (Agilent Technologies,
Santa Clara, CA, USA). The UHPLC system was equipped with a binary
pump with an integrated vacuum degasser (G4220A), an autosampler (G4226A),
and a thermostated column compartment (G1316C). The column used was
ZORBAX Extend-C18 (1.8 μm, 2.1 mm × 100 mm, Agilent Technologies,
Memphis, TN, USA), and peaks were resolved using a mobile phase A
(1% acetic acid in water) and a mobile phase B (1% acetic acid in
70:30 acetone/ACN). The gradient for elution was 0–4 min (3%
B), 4–5 min (50% B), 5–7 min (80% B), 7–8 min
(80% B), and 8–11 min (3% B) with a flow rate of 0.400 mL min–1. The injection volume was 100 μL. The column
temperature was held at 40 °C. All compounds were analyzed in
negative mode using multiple reaction monitoring (MRM). The source
conditions included the following: gas temperature at 350 °C;
gas flow at 9 L min–1; 30 psi for the nebulizer;
sheath gas flow is 12 L min–1 at 350 °C; capillary
voltage at (−)5000 V, and electron multiplier voltage at (−)2000
V. The dwell time was 50 ms, and the cell acceleration was at 7 V
for each transition.
using an Agilent 1290 Infinity UHPLC system interfaced to a 6460 triple-quadrupole
mass spectrometer with ESI via Jet Stream technology (Agilent Technologies,
Santa Clara, CA, USA). The UHPLC system was equipped with a binary
pump with an integrated vacuum degasser (G4220A), an autosampler (G4226A),
and a thermostated column compartment (G1316C). The column used was
ZORBAX Extend-C18 (1.8 μm, 2.1 mm × 100 mm, Agilent Technologies,
Memphis, TN, USA), and peaks were resolved using a mobile phase A
(1% acetic acid in water) and a mobile phase B (1% acetic acid in
70:30 acetone/ACN). The gradient for elution was 0–4 min (3%
B), 4–5 min (50% B), 5–7 min (80% B), 7–8 min
(80% B), and 8–11 min (3% B) with a flow rate of 0.400 mL min–1. The injection volume was 100 μL. The column
temperature was held at 40 °C. All compounds were analyzed in
negative mode using multiple reaction monitoring (MRM). The source
conditions included the following: gas temperature at 350 °C;
gas flow at 9 L min–1; 30 psi for the nebulizer;
sheath gas flow is 12 L min–1 at 350 °C; capillary
voltage at (−)5000 V, and electron multiplier voltage at (−)2000
V. The dwell time was 50 ms, and the cell acceleration was at 7 V
for each transition.
4
Quantifying Urinary Adherence Markers
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To measure urinary concentrations of the adherence marker compounds, urine samples were thawed at room temperature (25°C) for 2 h and diluted 10 (trans-resveratrol) or 50 (saccharin) times in methanol:water (1:1). Urine samples containing saccharin had 100 ng/mL of saccharin-d4 (CDN Isotopes) added, and were vortexed for 5 s. Samples were transferred to an autosampler vial for analysis by Agilent 1290 Infinity Ultra-HPLC (UHPLC) interfaced to a 6460 triple-quadrupole mass spectrometer (MS/MS) with electrospray ionization (ESI) via Jet Stream technology (Agilent Technologies) (15 (link)). Resveratrol metabolites were expressed as resveratrol equivalents. Recoveries for this method have been shown to be >80% at the range of concentrations used in this study.
5
Quantitative Analysis of Mycotoxins by UHPLC-MS/MS
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An Agilent 1290 series UHPLC system coupled to a 6460 Triple Quadrupole (QqQ) mass spectrometer (both Agilent Technologies, Waldbronn, Germany) was used to analyze the samples. Precursor and product ion selection as well as the optimization of collision energies were performed with flow injection of single-analyte solutions. UHPLC separations were performed in a reversed-phase C18 analytical column of 50 mm × 2.1 mm and 1.8 μm particle size (ZORBAX RRHD Eclipse Plus C18) by Agilent Technologies (Waldbronn, Germany).
The chromatographic solvents were water 0.1% formic acid solution (eluent A) and acetonitrile 0.1% formic acid (eluent B). The gradient program was as follows: 0.0 min, 10% B; 2.4 min, 42% B; 6.0 min, 51% B; 6.2 min, 95% B; 7.0 min, 10% B. A subsequent re-equilibration time (5 min) should be performed before next injection. The constant flow rate was 0.3 mL/min while the injection volume was 2 μL. Moreover, the column temperature was maintained at 30 °C.
MS/MS analyses of mycotoxins were performed on an 6460 QqQ mass spectrometer with Agilent Jet Stream Technology under the dynamic multiple reaction monitoring (DMRM) conditions in ESI+. The following settings were used: nebulizer, 40 psi; drying gas temperature, 350 °C; drying gas flow, 10 L/min; capillary voltage, 4000 V.
The chromatographic solvents were water 0.1% formic acid solution (eluent A) and acetonitrile 0.1% formic acid (eluent B). The gradient program was as follows: 0.0 min, 10% B; 2.4 min, 42% B; 6.0 min, 51% B; 6.2 min, 95% B; 7.0 min, 10% B. A subsequent re-equilibration time (5 min) should be performed before next injection. The constant flow rate was 0.3 mL/min while the injection volume was 2 μL. Moreover, the column temperature was maintained at 30 °C.
MS/MS analyses of mycotoxins were performed on an 6460 QqQ mass spectrometer with Agilent Jet Stream Technology under the dynamic multiple reaction monitoring (DMRM) conditions in ESI+. The following settings were used: nebulizer, 40 psi; drying gas temperature, 350 °C; drying gas flow, 10 L/min; capillary voltage, 4000 V.
6
Quantitative Analysis of Butanol Residues
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Analyses of the butanol residues of each plant were performed with UHPLC Agilent 1290 infinity system with a DAD G4212A and MS Agilent G6540A Q-TOF with Agilent Jet Stream technology electrospray ionization. Separation was performed on a Phenomenex Luna Omega column (C18, 1.9 u, 90 A°, 75 × 2.0 mm) using gradient mixtures of 0.1% formic acid (solvent A) and MeCN supplemented with 0.1% formic acid (solvent B). Gradient: 0.0−8.0 min, 0→30% B; 8.0−8.1 min, 30→98% B; 8.1−9.1 min, 98% B; 9.1−9.2 min, 98→5% B; 9.2−10.0 min, 5% B; flow rate, 0.6 mL/min; injection volume, 1 μL; oven temperature, 40 °C. Data analysis was performed by MassHunter Workstation Software Qualitative Analysis (B.07.00, B.10.00, Agilent) using automatic mass spectrum integration. LC-Q-TOF-MS/MS analyses were performed in positive and negative ionization modes to obtain maximum information on its composition. The metabolites were characterized based on their mass spectra, using the precursor ion and comparison of the fragmentation patterns with molecules described in the literature [15 (link)]. The putative identification of these compounds is summarized in Table 1 , where the compounds are listed according to their retention times in the total ion chromatogram (TIC).
7
Quantitative HAE Analysis by HR-LC/MS-MS
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To analyse HAE by HR-LC/MS-MS technique, facility was subcontracted to Sophisticated Analytical Instrument Facility (SAIF) - Indian Institute of Technology, Bombay (IIT Bombay), India. Here, firstly chromatographic separation was achieved using extra densely bonded and double end capped active silanols as stationary phase packed in Agilent Eclipse XDB-C-18 2.1 × 150 mm, 5 µm column and acetonitrile-water (with 0.1% formic acid) as mobile phase at flow rate of 0.2 ml/min. Then, LC-ESI-MS analysis of HAE was performed in dual (positive and negative) ion mode using Agilent Jet Stream technology with a hexabore capillary sampling array and dual-stage ion funnel for increased ion sampling and transmission where fragmentation achieved on collision induced dissociation (CID) by varying the collision cell voltage. Firstly chromatogram was obtained and studied for retention volume and column efficiency
8
Quantitative Analysis of Bile Acids by UHPLC-MS/MS
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Chromatographic analyses were performed in an Agilent 1290 Infinity LC Series coupled to a 6460 Triple Quadrupole (QqQ) mass spectrometer with an electrospray ionisation (ESI) interface, all from Agilent Technologies (Waldbronn, Germany), operating in positive ion mode. Chromatographic separation was performed using an Eclipse Plus C18 (100 mm × 2.1 mm, 1.8 μm) from Agilent Technologies (Waldbronn, Germany). In addition, 0.1% formic acid-water solution (eluent A) and 0.1% formic acid-acetonitrile solution (eluent B) were used as the mobile phase at a constant flow of 0.2 mL/min. Column temperature was set at 30 °C and the injection volume was 2 µL.
MS/MS analyses of BAs were performed on the 6460 QqQ mass spectrometer with Agilent Jet Stream Technology under the dynamic multiple reaction monitoring (DMRM) conditions in ESI+. The following settings were used: nebulizer, 45 psi; drying gas temperature, 300 °C; drying gas flow, 10 L/min; capillary voltage, 4000 V. Fragmentor voltage and collision energies were optimized for each analyte during infusion of the pure standard, and the most abundant fragment ion was chosen for the selected reaction monitoring (Table 1 ).
MS/MS analyses of BAs were performed on the 6460 QqQ mass spectrometer with Agilent Jet Stream Technology under the dynamic multiple reaction monitoring (DMRM) conditions in ESI+. The following settings were used: nebulizer, 45 psi; drying gas temperature, 300 °C; drying gas flow, 10 L/min; capillary voltage, 4000 V. Fragmentor voltage and collision energies were optimized for each analyte during infusion of the pure standard, and the most abundant fragment ion was chosen for the selected reaction monitoring (
9
Phenolic Composition Analysis of Green Coffee Beans
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A 1200 LC DAD system coupled to an Accurate Mass Quadrupole Time of Flight (Q ToF) detector with electrospray ionization (ESI) Jet Stream Technology (Agilent Technologies) was used to characterize the phenolic composition of the green coffee bean extracts. The chromatographic conditions and gradient elution were identical to those described above. Sample (1 L) was injected and compounds were separated on a Supherspher RP18 column (4 x 250 mm, 4 m particle size, Agilent Technologies) and detected at 280 and 320 nm. The MS was fitted with an atmospheric pressure ESI source which operated in negative ion mode.
The Q ToF operating conditions were as follows: 12 L/min dry gas flow at 350 ºC, 7 L/min sheath gas volume at 325 ºC, nebulizing pressure at 45 psig, capillary voltage at 3500 V, fragmentor and nozzle voltages at 100 and 0 V, respectively. Mass spectrometry data were acquired in the 100 1000 m/z range. Mass Hunter Workstation Software was used to process data.
The Q ToF operating conditions were as follows: 12 L/min dry gas flow at 350 ºC, 7 L/min sheath gas volume at 325 ºC, nebulizing pressure at 45 psig, capillary voltage at 3500 V, fragmentor and nozzle voltages at 100 and 0 V, respectively. Mass spectrometry data were acquired in the 100 1000 m/z range. Mass Hunter Workstation Software was used to process data.
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