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Pegfp c3 hyap1

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PEGFP-C3-hYAP1 is a plasmid vector that expresses a fusion protein of the green fluorescent protein (GFP) and the human Yes-associated protein 1 (hYAP1). This vector can be used for the expression and localization of the hYAP1 protein in mammalian cells.

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Lab products found in correlation

Pegfp c3 lats1, by Addgene (2 mentions) Lipofectamine 3000, by Addgene (1 mentions) Fugene hd transfection reagent, by Promega (1 mentions) Pgfp c3 vinculin, by Addgene (1 mentions) Cmv gfp nmhc 2 b, by Addgene (1 mentions) Pcdna3 p53 wt, by Addgene (1 mentions) Prl tk, by Promega (1 mentions)

4 protocols using pegfp c3 hyap1

1

Plasmid Construction and Characterization

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pEGFP-C3-hYAP1 and pEGFP C3-Lats1 (Addgene plasmid # 19053) were gifts from Marius Sudol (Addgene plasmid # 17843). HA-AMOT p130 was a gift from Kunliang Guan (Addgene plasmid # 32821) pcDNA3.1-ccdB-Nanoluc was a gift from Mikko Taipale (Addgene plasmid # 87067). GFP-ASPP2 was a gift from Charles D. Lopez.
Hennigan R.F., Thomson C.S., Stachowski K., Nassar N, & Ratner N. (2023). Merlin tumor suppressor function is regulated by PIP2-mediated dimerization. PLOS ONE, 18(2), e0281876.
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2

Stable YAP1 Gene Silencing in Prostate Cancer

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Stable YAP1 gene silencing in LNCaP and DU145 cells was performed using pGIPZ-based short hairpin RNA (shRNA) vectors for human YAP1 (clone VGH5518–200174078, clone VGH5518–200185118, clone VGH5518–200279049 and clone VGH5518–200282387 (Dharmacon, Lafayette, CO, USA)), using DharmaFECT kb transfection reagent (GE Healthcare, Chicago, IL, USA # T-2006–01), following which, stable YAP1 knockdown clones were generated using puromycin selection. H660 cells were transiently transfected with YAP1 (pEGFP-C3-hYAP1 [#17843, Addgene, Watertown, MA, USA]) using Lipofectamine 3000 as previously described [20 (link)].
Asrani K., Torres A.F., Woo J., Vidotto T., Tsai H.K., Luo J., Corey E., Hanratty B., Coleman I., Yegnasubramanian S., De Marzo A.M., Nelson P.S., Haffner M.C, & Lotan T.L. (2021). Reciprocal YAP1 loss and INSM1 expression in neuroendocrine prostate cancer. The Journal of pathology, 255(4), 425-437.
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3

Overexpressing Yap and α-catenin in Cells

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Briefly, 2x105 cells were seeded into a 6-well plate overnight before transfected with α-catenin-GFP (gift from Ann Wheeler) or pEGFP-C3-hYAP1 (Addgene Plasmid #17843) or pENTR1A-Yap S127A (Addgene, Plasmid #46050) expression vector along with an empty vector plasmid (pBABE-puro served as the control) using FuGENE HD transfection reagent (E2311, Promega). Cells were re-seeded on coverslips the next day in 24-well plates overnight before being treated with or without hydrogen peroxide according to the experiments.
Huang Y., Jedličková H., Cai Y., Rehman A., Gammon L., Ahmad U.S., Uttagomol J., Parkinson E.K., Fortune F, & Wan H. (2021). Oxidative Stress-Mediated YAP Dysregulation Contributes to the Pathogenesis of Pemphigus Vulgaris. Frontiers in Immunology, 12, 649502.
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4

Plasmid Sourcing for Cell Biology

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mApple-N1 (Addgene plasmid no. 54567) was a gift from M. Davidson. pSK ZO-1 (Addgene plasmid no. 30316) was a gift from J. Anderson and A. Fanning, CMV-GFP-NMHC II-B (Addgene plasmid no. 11348) was a gift from R. Adelstein, pGFP(C3)-vinculin (Addgene plasmid no. 30312) was a gift from K. Hahn, pKvenus-Scrib (Addgene plasmid no. 58738) was a gift from I. Macara, and pEGFP-C3-hYAP1 and pEGFP C3-Lats1 (Addgene plasmid nos. 19053 and 17843, respectively) were gifts from M. Sudol. HA-AMOT p130 (Addgene plasmid no. 32821) was a gift from K. Guan. pcDNA3.1-ccdB-NLuc (Addgene plasmid no. 87067) was a gift from M. Taipale. GFP-afadin was a gift from Y. Takai (107 (link)). GFP-ASPP2 was a gift from C. D. Lopez (108 (link)). p4XBS2WT-Luc (Addgene plasmid no. 16593) was a gift from B. Vogelstein. p8xGTIIC-luciferase (Addgene plasmid no. 34615) was a gift from S. Piccolo. pcDNA3 p53 WT (Addgene plasmid no. 69003) was a gift from D. Meek. Plasmids containing the open reading frames for Epb4.1l3 and Rassf8 from the Human ORFeome V1 were purchased from the DNASU Plasmid Repository at the Arizona State University. pRL-TK was purchased from Promega. The Tet-inducible plasmids, pTRE3G-BI-ZsGreen and pCMV-Tet3G, were purchased from Tanaka Holdings.
Hennigan R.F., Fletcher J.S., Guard S, & Ratner N. (2019). Proximity biotinylation identifies a set of conformation-specific interactions between Merlin and cell junction proteins. Science signaling, 12(578), eaau8749.
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