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Anti high mobility group box 1 hmgb1

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Anti-High Mobility Group Box I (HMGB1) is a primary antibody used for the detection and quantification of HMGB1 protein in various applications such as Western blotting and immunohistochemistry. HMGB1 is a nuclear protein involved in the regulation of transcription and chromosomal stability.

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Lab products found in correlation

Anti cleaved caspase 3, by Cell Signaling Technology (2 mentions) Anti calreticulin, by Abcam (2 mentions)

Spelling variants of this product

HMGB1 (192 citations) Anti-HMGB1 (70 citations)

2 protocols using anti high mobility group box 1 hmgb1

1

Immunohistochemical Analysis of Excised Tumors

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Excised tumors fixed in 10% neutral buffered formalin (Azer Scientific, Morgantown PA, USA) were paraffin embedded, sectioned into 5- micron slices, and stained with anti-cleaved caspase 3 (Cell Signaling, Danvers MA, USA), anti-High Mobility Group Box I (HMGB1; Abcam, Cambridge UK), or anti-Calreticulin (Abcam), followed by secondary antibody (Vector Laboratories, Newark CA, USA) and counter stained with hematoxylin. Marker expression was determined by Aperio Image Scope algorithm.
Uccello T.P., Lesch M.L., Kintzel S.A., Gradzewicz L.B., Lamrous L., Murphy S.P., Fleming F.J., Mills B.N., Murphy J.D., Hughson A., Hannon G., Garrett-Larsen J., Qiu H., Drage M.G., Ye J., Gavras N.W., Keeley D.C., Love T.M., Repasky E.A., Lord E.M., Linehan D.C, & Gerber S.A. (2023). New insights into the responder/nonresponder divide in rectal cancer: Damage-induced Type I IFNs dictate treatment efficacy and can be targeted to enhance radiotherapy. Cell Death & Disease, 14(7), 470.
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2

Immunohistochemical Analysis of Excised Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Excised tumors fixed in 10% neutral buffered formalin (Azer Scientific, Morgantown PA, USA) were paraffin embedded, sectioned into 5- micron slices, and stained with anti-cleaved caspase 3 (Cell Signaling, Danvers MA, USA), anti-High Mobility Group Box I (HMGB1; Abcam, Cambridge UK), or anti-Calreticulin (Abcam), followed by secondary antibody (Vector Laboratories, Newark CA, USA) and counter stained with hematoxylin. Marker expression was determined by Aperio Image Scope algorithm.
Gerber S., Uccello T., Lesch M., Kintzel S., Gradzewicz L., Lamrous L., Murphy S., Fleming F., Mills B., Murphy J., Hughson A., Garrett-Larsen J., Qiu H., Drage M., Ye J., Gavras N., Keeley D., Love T., Repasky E., Lord E, & Linehan D. (2023). New insights into the responder/nonresponder divide in rectal cancer: Damage-induced Type I IFNs dictate treatment efficacy and can be targeted to enhance radiotherapy. Research Square.
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