Prim v7

All raw data were stored in a computerized database (MS Excel 2016, Microsoft, Redmond, WA, USA). Statistical analysis and data visualization were performed by using R-Studio v1.2.5042 (RStudio: Integrated Development for R. RStudio, Boston, MA, USA) and GraphPad Prim v7 (GraphPad Software, San Diego, CA, USA). Data are presented as n (%) or median (IQR). Comparisons between the healthy control and dengue group were performed by Mann–Whitney U test. Scatter plots were used to visualize the distribution of the data within the two groups. A p-value of < 0.05 was considered significantly different by statistical analysis. General statistics in this study were provided in Supplementary Table S2.

All raw data were stored in a computerized database (MS Excel 2016, Microsoft, Redmond, Washington, USA). Descriptive statistics were calculated for all patients in this study and summarized in Table 1. Statistical Analysis was performed using R- Studio v1.2.5042 (RStudio: Integrated Development for R. RStudio, Boston, MA, USA) and Graph-Pad Prim v7 (Graphpad Software, San Diego, CA, USA) Kruskal–Wallis test was performed on all groups, whereas the Mann–Whitney test was used to compare groups head to head. Boxplots and scatter plots were used to visualize the distribution of the cytokine data within the different groups or for the different days of fever. A p-Value < 0.05 was considered as significantly different by statistical analysis. Receiver operating characteristic (ROC) curves were used to assess the diagnostic values in discriminating dengue fever and disease severity. The optimal diagnostic cutoff value was determined according to Youden’s J-statistic, and the relative sensitivity, specificity, positive and negative predictive values were calculated.

The analysis was performed using GraphPad Prim v.7 (GraphPad Software, CA, USA). All experimental data are presented as mean ± standard error of the mean (SEM).