Following fixation, sections were rinsed in 175 mM PB for 4 hr, then a buffered glycine solution (50 mM glycine in 175 mM PB) for 16 hr, and then rinsed for 6 hr in 175 mM PB. Regions of 300-μm-thick coronal sections were then trimmed down to volumes of approximately 2 × 2 × 0.3 mm3 with a scalpel for cortical and hippocampal regions; olfactory bulb sections were left intact. For labeling of NeuN-positive somata (Figures 1 and 5a, b, e, f ), sections were incubated in the ABS with Alexa Fluor-488 conjugated anti-NeuN (Abcam) for 72 hr. For labeling of CB+ and CR+ somata (Figures 3a–b and 5c, d, g, h ), sections were simultaneously incubated in guinea pig anti-CB (Synaptic Systems) and rabbit anti-CR (Sigma) for 72 hr, rinsed in 175 mM PB for 12 hr, and then incubated in the secondary antibodies, DyLight-405 goat anti-guinea pig IgG F(ab’)2 fragment (Jackson ImmunoResearch) for CB and Alexa Fluor-594 donkey anti-rabbit IgG F(ab’)2 fragment (Jackson ImmunoResearch) for CR, for 48 hr.
Rabbit anti cr
Manufactured by Merck Group
Rabbit anti-CR is a polyclonal antibody raised against the CR (complement receptor) antigen. It is designed to detect and bind to the CR protein. This antibody can be used in various immunological applications to study the expression and distribution of the CR protein.
Automatically generated - may contain errors
Lab products found in correlation
Guinea pig anti cb, by Synaptic Systems (2 mentions)
Dylight 405 goat anti guinea pig igg f ab 2 fragment, by Jackson ImmunoResearch (2 mentions)
Alexa fluor 594 donkey anti rabbit igg f ab 2 fragment, by Jackson ImmunoResearch (2 mentions)
Alexa fluor 488 conjugated anti neun, by Abcam (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594, by Thermo Fisher Scientific (1 mentions)
Mouse anti pv, by Merck Group (1 mentions)
Rabbit anti chat, by Merck Group (1 mentions)
Rabbit anti npy, by Abcam (1 mentions)
Mouse anti parv, by Merck Group (1 mentions)
Peroxidase anti peroxidase complex, by Merck Group (1 mentions)
Rabbit anti darpp 32, by Cell Signaling Technology (1 mentions)
Mouse anti neun, by Merck Group (1 mentions)
4 protocols using rabbit anti cr
1
Immunohistochemical Labeling of Neuronal Subtypes
2
Multicolor Immunolabeling of Neuronal Subtypes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following fixation, sections were rinsed in 175 mM PB for 4 hr, then a buffered glycine solution (50 mM glycine in 175 mM PB) for 16 hr, and then rinsed for 6 hr in 175 mM PB. For labeling of CB+, CR+, and TH+ somata (Figure 6a ), sections were first simultaneously incubated with guinea pig anti-CB (Synaptic Systems) and rabbit anti-CR (Sigma) for 72 hr, then rinsed in 175 mM PB for 72 hr. Sections were then simultaneously incubated with DyLight-405 goat anti-guinea pig IgG F(ab’)2 fragment (Jackson ImmunoResearch) for CB, Alexa Fluor-594 donkey anti-rabbit IgG F(ab’)2 fragment (Jackson ImmunoResearch) for CR, and the primary antibody Alexa Fluor-488 conjugated anti-TH, clone LNC1 (EMD Millipore) for 72 hr.
3
Immunohistochemistry of Vibratome Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Free-floating vibratome sections (50 μm) were processed according to our published protocol32 (link),50 (link). Primary antibody dilutions were as follows: chicken anti-GFP (1:500; Aves); mouse anti-PV (1:500; Sigma); rabbit anti-SST (1:200; Santa Cruz); rabbit anti-CR (1:1000; Millipore). Secondary antibodies included Alexa Fluor 488 and Alexa Fluor 594 (1:500; Life Technologies).
4
Immunohistochemical Staining of Brain Regions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Brain sections were pre-treated with 0.3% H2O2 in 10 mM PBS (pH 7.4, 4°C) for 30 min. Separate series of sections were respectively incubated at 4°C for 48 h with one of the following primary antibodies: mouse anti-NeuN (1∶500, Millipore), rabbit anti-Darpp32 (1∶200, Cell Signaling, Danvers, MA), mouse anti-Parv (1∶1,000, Sigma), rabbit anti-Cr (1∶2,000, Millipore), rabbit anti-NPY (1∶5,000, ABCAM) and rabbit anti-ChAT (1∶1,000, Millipore). After rinsed in 10 mM PBS for three times (5 min/time), the sections were applied with secondary antibodies anti-mouse IgG or anti-rabbit IgG (both 1∶200, Sigma) at room temperature for 4 h, followed by three rinses (5 min/time) in 10 mM PBS and incubation with homologous peroxidase-antiperoxidase (PAP) complex (1∶200, Sigma) at room temperature for 2 h. The peroxidase reaction was performed using 3, 3′-diaminobenzidine (DAB, 0.05% in 10 mM PBS, pH 7.4, Sigma) for 2–8 min, and then the sections were mounted onto gelatin-coated slides, routinely dehydrated, cleared and covered with neutral balsam for microscopic detection.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!