Cytokine levels of IL-1β, IL-6, IFN-γ, MCP-1 and sFlt-1 in the serum of each maternal rat and the activity levels of nitric oxide (NO) and inducible NO synthase (iNOS) in the homogenate of placenta tissue were determined by ELISA with rat ELISA kits IL-1β (ml003057), IL-6 (ml102828), IFN-γ (ml064291), MCP-1 (ml002960), sFlt-1 (ml059017), NO (ml059000) and iNOS (ml003127) purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. according to the manufacturer's protocols. The absorbance in each group was detected using a microplate reader at 450 nm.
Ifn γ
Manufactured by Shanghai Enzyme-linked
Sourced in China
IFN-γ is an enzyme-linked immunosorbent assay (ELISA) kit used for the quantitative detection of interferon-gamma in various sample types. It is a sensitive and specific tool for measuring this important cytokine.
Automatically generated - may contain errors
Lab products found in correlation
Il 1β, by Shanghai Enzyme-linked (18 mentions)
Interleukin 6 (il 6), by Shanghai Enzyme-linked (18 mentions)
Tnf α, by Shanghai Enzyme-linked (9 mentions)
Il 10, by Shanghai Enzyme-linked (9 mentions)
Il 18, by Shanghai Enzyme-linked (9 mentions)
Tgf β, by Shanghai Enzyme-linked (9 mentions)
Elisa kit, by Shanghai Enzyme-linked (9 mentions)
P iκbα, by Affinity Biosciences (3 mentions)
Nlrp3, by Affinity Biosciences (3 mentions)
Histone h3, by Affinity Biosciences (3 mentions)
Gapdh, by Affinity Biosciences (3 mentions)
Erythromycin, by Meilun (3 mentions)
Mcp 1, by Shanghai Enzyme-linked (3 mentions)
Sflt 1, by Shanghai Enzyme-linked (3 mentions)
Total rna extraction kit, by Promega (3 mentions)
3 protocols using ifn γ
1
Maternal Cytokine and Placental NO Levels
2
Biochemical Assays and Reagents Protocol
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COP (purity >98%) was purchased from Shanghai Yuan Ye Biotechnology Co., Ltd (Shanghai, China). Dextra sulfate sodium was obtained from MP Biomedical (Irvine, CA, USA). Cefadroxil (purity >99%), terramycin (purity >98%) and erythromycin (purity >99%) were purchased from Dalian Meilun Biotechnology Co., Ltd (Dalian, China). Mesalazine (MSZ) was obtained from Losan Pharma GmbH, Germany. ELISA kits (TNF-α, IL-6, IL-10, IL-18, IL-1β, TGF-β, and IFN-γ) were obtained from Shanghai Enzyme-linked Biotechnology Co., Ltd (Shanghai, China). BCA kit and myeloperoxidase (MPO) assay kit were obtained from the Jiancheng Bioengineering Institute of Nanjing (Nanjing, Jiangsu, China). Primary antibodies against p65, IκBα, p-IκBα, NLRP3, ASC, Capase-1, Caspase-1 p10, GAPDH and Histone H3 were purchased from Affinity Biosciences (OH, USA). All other reagents and chemicals were at least of analytical grade.
3
Commercial FFC Solution Evaluation for Biological Research
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Commercial FFC solution (clinical over-the-counter veterinary medicine, purity ≥10%) were purchased from Shenniu Biological Technical Co., Ltd. (Dezhou, China).
Total RNA extraction kit was purchased from Promega Biotechnology Co., Ltd. (Beijing, China). PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) and SYBR Premix Dimer Eraser reagent Kit (Perfect Real Time) were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). Hematoxylin and eosin dyes were purchased from Biotopped life sciences Co., Ltd. (Beijing, China). ELISA kits of IL-1β, IL-2, TNF-α, IFN-γ, and CXCL-10 were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. (Shanghai, China). In Situ Cell Death Detection Kit (POD) was purchased from Roche Diagnostics Co., Ltd. (Mannheim, Germany).
Total RNA extraction kit was purchased from Promega Biotechnology Co., Ltd. (Beijing, China). PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) and SYBR Premix Dimer Eraser reagent Kit (Perfect Real Time) were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). Hematoxylin and eosin dyes were purchased from Biotopped life sciences Co., Ltd. (Beijing, China). ELISA kits of IL-1β, IL-2, TNF-α, IFN-γ, and CXCL-10 were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. (Shanghai, China). In Situ Cell Death Detection Kit (POD) was purchased from Roche Diagnostics Co., Ltd. (Mannheim, Germany).
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