C6198

Manufactured by Abcam

Sourced in United States

C6198 is a laboratory equipment product. It serves the core function of enabling scientific research and experimentation.

Automatically generated - may contain errors

Lab products found in correlation

Ab13970, by Abcam (1 mentions) Ab5320, by Abcam (1 mentions) Ab4680, by Abcam (1 mentions) Ab7778, by Merck Group (1 mentions) Ab32570, by Merck Group (1 mentions) Mca497g, by Thermo Fisher Scientific (1 mentions) Ab150113, by Abcam (1 mentions) Ab3526, by Abcam (1 mentions) Ab6717, by Abcam (1 mentions)

3 protocols using c6198

Immunofluorescence Markers of Neural Tissue

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The following primary antibodies were used for IF at the indicated dilutions: anti-P0 (1/500, Abcam, ab39375), anti-p75 (1/500, Millipore, ab1554), anti-S100 (1/1000, Dako, Z0311), anti-Iba1 (1/500, Wako, 019-19741), anti-CD31 (1/100, BD Biosciences, 553370), anti-neurofilament 200 kD (1/1000, Abcam, ab4680), anti-laminin (1/500, Abcam, 11575), anti-collagen III (1/1000, Abcam, ab7778), anti-fibronectin (1/500, Sigma-Aldrich, clone FN-3E2), anti-NG2 (1/500, Millipore, ab5320), anti-PDGFRβ (1/500, Abcam, ab32570), anti-αSMA (1/1000, Sigma-Aldrich, C6198), anti-GFP (1/1000, Abcam, ab13970), anti-Glut1 (1/500, Abcam, ab652), anti-F4/80 (1/100, Bio-Rad, MCA497G) and anti-NG2 (1/100, Thermo Fisher Scientific, MA5-24247). For further details of primary antibodies used in this study, see Table S1.

Stierli S., Napoli I., White I.J., Cattin A.L., Monteza Cabrejos A., Garcia Calavia N., Malong L., Ribeiro S., Nihouarn J., Williams R., Young K.M., Richardson W.D, & Lloyd A.C. (2018). The regulation of the homeostasis and regeneration of peripheral nerve is distinct from the CNS and independent of a stem cell population. Development (Cambridge, England), 145(24), dev170316.

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Immunostaining of Primary Fibroblasts

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For in vitro IHC staining of primary fibroblasts, the media was aspirated and the cells were washed two times with PBS. The fibroblasts were then fixed in 4% paraformaldehyde (in PBS) for 15 min at room temperature, followed by 15 min incubations in 100% methanol, and then 70% ethanol. After rinsing in PBS, the cells were blocked in 6% Bovine Serum Albumin (BSA; Sigma Aldrich A3059) for at least an hour at room temperature or overnight at 4°C. Following blocking, cells were washed three times with PBS for 10 min at room temperature and incubated in primary antibody (anti-αSMA, Sigma, C6198) or anti-HuR, Abcam, ab28660) at 1:200 in 0.6% BSA for at least 2 hours at room temperature or overnight at 4°C. Cells were then incubated in AlexaFluor 488 anti-mouse secondary antibody (Abcam, ab150113) diluted 1:200 in 0.6% BSA for an hour at room temperature, washed 3 times with PBS, and incubated in Hoescht 33342 nuclear stain (1:3000 in PBS) for imaging of the nuclei.

Green L.C., Slone S., Anthony S.R., Guarnieri A.R., Parkins S., Shearer S.M., Nieman M.L., Roy S., Aube J., Wu X., Xu L., Kanisicak O, & Tranter M. (2022). HuR-dependent expression of Wisp1 is necessary for TGFβ-induced cardiac myofibroblast activity. Journal of molecular and cellular cardiology, 174, 38-46.

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Adipogenesis and Fibrogenesis Quantification

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The FAPs were stained with goat-anti-mouse perilipin A (1:1000, ab3526, Abcam, CA, USA) or preconjugated mouse-anti-mouse Cy 3 alpha smooth muscle actin (αSMA, 1:500, c6198. Sigma-Aldrich, MO, USA) and then stained with Donkey-anti-Goat FITC (1:2000, ab6717, Abcam, CA, USA). DAPI was added to the mounting medium to stain the nuclei. The adipogenesis index was determined by the percentage of cells expressing perilipin over the total number of nuclei in each image. The fibrogenesis index was determined by the percentage of cells expressing αSMA over the total number of nuclei in each image.

Liu M., Feeley B.T., Kim H.T, & Liu X. (2022). The Role of Matrix Metalloproteinase-13 (MMP13) in TGFβ/BMP Pathway Regulation of Fibro-Adipogenic Progenitor (FAP) Differentiation. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 56(6).

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