Db 5ms 5 phenyl methyl siloxane column

The GC–MS analysis was performed following the procedure reported by Murugesu et al. [5 (link)], with some modifications. One microliter of the derivatized samples was injected in the splitless mode into a GC–MS system, consisting of an Agilent 6890 gas chromatograph and an HP 5973 mass selective detector (Agilent Technologies, Santa Clara, CA, USA). The extracts were separated on a DB-5MS 5% phenyl methyl siloxane column with an inner diameter (ID) of 250 µm and a film thickness of 0.25 µm (Agilent Technologies, Santa Clara, CA, USA) using helium as the carrier gas at a flow rate of 1 mL/min. The initial oven temperature was set to 100 °C for 5 min, and then increased sequentially to a target temperature of 190 °C at a rate of 10 °C/min, then to 204 °C at a rate of 1 °C/min and eventually to 325 °C at a rate of 2 °C/min with a total run time of 88.5 min. The injector and ion source temperatures were set to 250 °C and 230 °C, respectively. Mass spectra were acquired using a full scan mode with a mass range of 50 to 550 amu. The detector was set to a solvent delay of 6 min.

GC-MS analysis was accomplished following the protocol described in Reference [51 (link)] with slight modification. The derivatized sample (µL) was injected in the splitless mode into the GC-MS system, which consisted of an Agilent 6890 GC-MS and an HP 5973 mass selective detector. The DB-5MS 5% phenyl methyl siloxane column with an inner diameter (ID) of 250 μm and a film thickness of 0.25 μm (Agilent Technologies Inc, Santa Clara, CA, USA) was used. The initial oven temperature was set to 85 °C, and then increased to a target temperature of 315 °C at a rate of 2 °C/min with a total running time of 120 min. Helium was used as the carrier gas with a flow rate of 1 mL/min. The injector and ion source temperatures were set to 250 and 280 °C, respectively. Mass spectra were acquired using a full scan and a monitoring mode with a mass scan range of 50 to 550 m/z. The spectra for each of the chromatogram peaks were compared with those in the NIST14 database library. The chromatogram and mass spectra were processed using an Agilent ChemStation, Automated Mass Spectral Deconvolution and Identification System and Agilent’s Deconvoluted Reporting Software (Agilent Technologies Inc, Santa Clara, CA, USA).