Glu p 2

White sesame seed was purchased from a local supermarket. The five adsorbents of activated carbons used in the present study were as follows: powdered type activated carbon made from peat (AP, F10), powdered type activated carbon made from wood (PW), powdered type activated carbon made from coconut shell (PC) and granular type activated carbon made from coconut shell (GC). F10, PW, PC and GC were purchased from Xin Sen Carbon Co., Ltd. (Nanping, China). AP was obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Acetonitrile (HPLC grade) was purchased from Thermo Fisher Scientific (Shanghai, China). Ammonium hydroxide and hydrochloric acid (HPLC grade) were obtained from Kemiou Chemical Reagent Co., Ltd. (Tianjin, China). Methyl alcohol, acetic acid and n-hexane were of HPLC grade, and other chemicals were of analytical reagent grade. Oasis MCX solid-phase extraction cartridge (150 mg, 6 mL) was purchased from Waters (Milford, CT, USA). Additionally, the water used was Wahaha purified water purchased from a local supermarket. The standards AαC, MeAαC, DMIP, Trp-P-1, Trp-P-2, Glu-P-2, MeIQ, MeIQx, IQ, PhIP, 4,8-DiMeIQx, 7,8-DiMeIQx were purchased from Toronto Research Chemicals (Toronto, ON, Canada). Harman, Norharman and 4,7,8-TriMeIQx were purchased from Alta scientific (Tianjin, China).

A total of 3 kg of raw pork spareribs (120 pieces) was procured from a local market located in New Taipei City, Taiwan. A total of 21 HA standards, including DMIP, IFP, PhIP, Phe-P-1, MeIQ, 4,8-DiMeIQx, 8-MeIQx, 7,8-DiMeIQx, IQx, IQ, IQ[4,5-b], Iso-IQ, MeAαC, AαC, Trp-P-2, Trp-P-1, Glu-P-2, Glu-P-1, Norharman, Harman, and internal standard 4,7,8-TriMeIQx were procured from Toronto Research Chemicals (Downsview, ON, Canada), while 24 PAH standards including DBaiP, DBahP, CcdP, DBahA, DBaeP, DBalP, BghiP, MCH, IP, CHR, BjF, BaA, BbF, BaP, BcF, Phe, Flu, Pyr, FL, Ant, AcP, NaP, AcPy, and internal standard Triphenylene were from Sigma-Aldrich Co. (St Louis, MO, USA). The full names of HA and PAH standards are shown in Tables S1 and S2, respectively.
An Acquity UPLC BEH C18 column (100 × 2.1 mm ID, particle size 1.7 μm) used for HA separation was from Waters Co. (Milford, MA, USA), while a DB-5 MS capillary column (30 m × 0.25 mm ID, film thickness 0.25 μm) for PAH separation was from Agilent Technologies Co. (Palo Alto, CA, USA). The QuEChERS extraction and purification kits were from Yu-Ho Co (New Taipei City, Taiwan). The HPLC-grade solvents including methanol, acetonitrile, acetone, and hexane were from Merck (Darmstadt, Germany).