The single known specimen is preserved as part and counterpart and required special imaging methods for viewing and documentation. A broad habitus view of the entire specimen was provided by combining separate images into a composite form. Each component image was taken from separate focal planes that subsequently were integrated by CombineZM® from a series of images. These different images then were stitched with Adobe Photoshop® CS 3 software under cross-polarized light with a Canon Rebel T3i camera equipped with a MP-E 65 mm lens and a MT 24 EX Canon Twin Flash. For comparison of the specimen to earlier studies that used standard light-microscopy, fully reflected vertical light as well as directed side light from different angles was used. In order to document specimen relief, two techniques were applied. The first approach was stereo-imaging that employed the Canon Rebel T3i camera. A second procedure involved virtual surface reconstruction resulting from stacks of images recorded on a Zeiss Axiophot microscope attached to a Skopetech DCM 510 camera. Cross-polarized light was provided by multiple, optical-fiber light sources. Virtual surfaces were calculated in Image Analyzer® software.
Rebel t3i camera
Manufactured by Canon
Sourced in Japan
The Canon Rebel T3i is a digital single-lens reflex (DSLR) camera. It features a 18.0-megapixel CMOS image sensor and a DIGIC 4 image processor. The camera can record 1080p Full HD video with stereo sound. It has a 3.0-inch articulating LCD screen and supports various EF and EF-S lenses.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using rebel t3i camera
1
Imaging Methods for Specimen Documentation
2
Yeast Growth Inhibition Assay
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We spotted 1 OD600/ml of log-phase yeast grown under selective conditions to maintain plasmids (either SD-Leu or YPD + Hyg B) in 10× serial dilution on selective plates containing inhibitors or DMSO as a control, incubated them at 30°C for 3 d, and imaged them with a Canon Rebel T3I camera (Canon, Tokyo, Japan).
3
Optical Characterization of Functional Materials
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Absorption measurements were performed using a Hewlett-Packard 8452A Diode Array spectrophotometer, using a cuvette filled with pure solvent as the blank. For PL spectroscopy, we used the 405-nm ion line of a diode laser operated at an excitation power density of ~1250Wm-2 as the excitation source. The PL was collected by an optical fiber (numerical aperture ~0.22) and directed to an Ocean Optics USB 2000+ miniature spectrometer. The efficiency of the system was corrected with an Ocean Optics calibrated light source. Photographs of the PE layers were taken with a Canon Rebel T3i camera.
4
Blood Cell Velocity Measurement
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A Canon Rebel T3i camera and Canon 100 mm macro lens mounted on a copy stand were used to obtain images of the entire animal. Higher magnifications, required to follow moving blood cells, was obtained using a Meiji stereoscopic microscope and the Canon camera. Images were processed using Adobe Photoshop software with any image enhancement applied uniformly to the entire frame. The velocity of blood cells was determined by measuring position of a selected cell in sequential frames from a video. Video files were converted to individual images using QuickTime Player 7 (Apple) and the positions of cells were determined using Photoshop (Adobe). The default configuration of Photoshop gives x,y positions to 0.1 pixel, which is useful when one wants to specify the center of an object of 3–16 pixels. There are many other combinations of applications that could obtain similar data. In this report velocity is defined as distance/time, where time is 1/30 s, the time between each video frame, and data in graphs representing a moving average over 5 time points.
5
Photographic Documentation of Crustacean Specimens
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All eight specimens were photographed using a Canon Rebel T3i camera with a MP-E 65 mm macro lens. Light was provided by a Canon Macro Twin Flash MT 24EX or a MeiKe FC 100 LED ring light. Light sources were equipped with polarization filters. A cross-polarized filter was placed in front of the lens. Cross-polarized light reduces reflections and enhances colour contrast (e.g., [16 ] and references therein). Additionally, one of the eight specimens (ZMUC-CRU-8679) was documented in 70 % ethanol using a Keyence BZ-9000 fluorescence microscope with either a 2×, 4× or 10× objective (resulting in approximately 20×, 40×, and 100× magnification, respectively; in a few cases the zoom function of the camera was also employed) depending on the different sizes of the body parts. UV light (377 nm) was used for illumination, using the autofluorescence capacities of the specimens (e.g. [17 (link)]). For high-resolution images, composite imaging was applied [18 , 19 ].
6
Quantitative Yeast Colony Imaging
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Strains containing a chromosomally integrated GFP-Snc1-Suc2 reporter were grown overnight in synthetic medium with 2% fructose (SF). Overnight cultures were adjusted to 0.1 OD600/ml, spotted on SF plates, and incubated at 30°C for 24 h. Images were taken before and after incubation with overlay reagents (125 mM sucrose, 100 mM NaOAC, pH 5.5, 0.4 mM N-ethylmaleimide, 0.01 mg/ml horseradish peroxidase, 8 U/ml glucose oxidase, 0.6 mg/ml O-dianisidine, and 0.7% agarose) using a Canon Rebel T3I camera (Canon, Tokyo, Japan). Densitometry of each colony was assessed using the Cell Profiler MeasureObjectIntensity module, and the intensity of each set of four was averaged for each replicate.
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