Pierce chemiluminescent nucleic acid detection module kit
The Pierce Chemiluminescent Nucleic Acid Detection Module Kit is a laboratory equipment used for the detection and quantification of nucleic acids through chemiluminescence technology. The kit includes the necessary reagents and components to perform this analysis.
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6 protocols using pierce chemiluminescent nucleic acid detection module kit
Northern Blot Analysis of miR-34a
Northern Blot Analysis of Plant mRNA
Southern Blot Analysis of mtpA Disruption
RNA-Protein Interactome Identification
Proteins binding to the respective transcripts were isolated employing streptavidin magnetic beads (Thermo Scientific, Vienna, Austria) according to the manufacturer’s protocol. Briefly, beads were washed and supplemented with RNA Capture buffer. Then, 50 pmol biotin-labeled RNA was added to the beads, followed by an incubation for 30 min at RT with agitation. Protein-RNA binding buffer (Tris pH 7.5 20 mM, NaCl 50 mM, MgCl2 2 mM, Tween 0.1%(v/v)), 30% glycerol, and 20 μg of nuclear lysate were added to the beads and incubated for 60 min at 4 °C with agitation. RNA-binding protein complexes were collected and washed with wash buffer (50 mM NaH2PO4, 300 mM NaCl, 20 mM imidazole) and eluted in 50 mM ammonium acetate. Proteins were separated by SDS-PAGE and subsequently silver stained.
Detecting RNAs in Protein Complexes
Southern Blot Analysis of TDR Transformants
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