Heart tissues were harvested after 4 weeks. Then, the heart tissues were fixed in 4% paraformaldehyde (pH 7.4). After fixation and paraffin embedding, the cardiac tissues were cut into 5-μm-thick sections. The sections were stained with hematoxylin and eosin (Servicebio; China) to analyze the global heart morphology and inflammatory cell infiltration. The sections were stained with Masson’s trichrome (Servicebio; China) to evaluate cardiac fibrosis. The sections were scanned at 20-fold magnification on a high-resolution microscope (Leica; Japan).
High resolution microscope
Manufactured by Leica
Sourced in Japan
The high-resolution microscope from Leica is a powerful imaging tool designed to provide detailed, high-quality visual analysis of small-scale specimens. It features advanced optics and imaging capabilities that enable users to examine samples with exceptional clarity and resolution.
Automatically generated - may contain errors
5 protocols using high resolution microscope
1
Histological Analysis of Heart Tissue
2
Histological Analysis of Mouse Heart
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Mice were euthanized and euthanized, hearts and serum were collected as described [17 (link)]. In brief, hearts were harvested and fixed in 4% paraformaldehyde overnight, embedded in O.C.T. (SAKURA, 4583) with the apex toward the surface. Then cut into 5-μm-thick transversal sections serially. The sections were stained with hematoxylin and eosin (HE) to analyze the global heart morphology and inflammatory cell infiltration and stained with Masson’s trichrome to measure collagen deposits. The sections were scanned at 20-fold magnification on a high-resolution microscope (Leica, Japan), and the images of Masson staining of perivascular were captured at 40-fold magnification.
3
Cirsilineol's Impact on Osteoblast Differentiation
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To investigate whether cirsilineol affected bone formation and osteoblasts differentiation, MC3T3-E1 cells were used for further examination. Specifically, MC3T3-E1 cells (2 × 105 cells/well) were seeded into 6-well plates and then treated with different concentration of cirsilineol (0, 10, 20 μM) in osteogenic medium. Three days after osteogenic induction, MC3T3-E1 cells were fixed with 10% formaldehyde and then cultured with ALP staining kits. Another two weeks after osteogenic induction, MC3T3-E1 cells were incubated with 0.1% alizarin red dye for 30 min. After that, all plates were photographed using a high‐resolution microscope (Leica, Germany).
4
Histological Analysis of Fish Tissues
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After the fish dissection, portions of tissues (gills and muscles) were preserved in 10% formalin for histological studies. The preserved tissues were processed in various grades of ethanol, cleared in xylene, and impregnated with wax (mp; 58°C). Five-micron-thick sections were cut using a rotary microtome (Leica RM 2165) at 100x. Tissue sections were stained with hematoxylin and eosin (H&E). Stained slides were observed and photographed under a high-resolution microscope (Lecia, Japan) fitted with a digital camera [33 (link)].
5
Histological and Ultrastructural Analysis of Mouse Hearts
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The hearts of mice were removed and placed in 4% paraformaldehyde (ph 7.4) and then embedded in paraffin wax. The sections were stained by Masson staining and HE staining and then histologically observed under a high-resolution microscope (Leica, Japan). And the heart tissue specimens used for electron microscopic observation were first fast fixed with 2.5% glutaraldehyde in 0.1 m sodium phosphate (pH 7.2), washed in the same buffer at 4 °C for 1 h, and fixed with 1% osmium tetroxide in sodium phosphate at 4 °C for 1 h. Each step lasted 10 min starting from 50%, followed by 2 changes of propylene oxide. These tissues were then immersed in Araldite. Ultrathin sections were stained using magnesium uranyl acetate and lead.
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