B6 sjl male mice

Mice used in this study were group housed (up to 5 mice of the same sex) with unrestricted access to water and standard chow diet, unless otherwise indicated, under specific pathogen free conditions in the Rockefeller University (RU) Comparative Bioscience Center. Mice used in this study ranged from 6 to 14 weeks old. Wild-type C57BL/6J and B6.SJL male mice were purchased from Jackson Laboratories. B1-8^hi, B1-8^hi DEC205^−/−, B1-8^hi CFP, and B1-8^hi c-Myc-GFP have been described^{6 (link),20 (link),75 (link)}. BTK^C481S point mutation mice were generated by microinjection of gRNA, hCas9, and single-stranded donor oligonucleotides into B6 zygote pronuclei (RU CRISPR and Genome Editing Center, RU Transgenic and Reproductive Technology Center)^{76 (link)}. Mutants were backcrossed to B6.SJL for 5+ generations to remove possible CRISPR off-target effects. B1-8^hi BTK^C481S and B1-8^hi DEC205^−/− BTK^C481S were generated by crossing to B1-8^hi and B1-8^hi DEC205^−/−. All experiments conform to protocols approved by the RU Institutional Animal Care and Use Committee.

CF1 female mice (Envigo), B6SJL male mice (Jackson Laboratory), and CD1 vasectomized male mice (Charles River) were housed in polysulfone cages in a pathogen-free facility on a 12-12 light-dark cycle. All animals had access to ad libitum water and standard chow (Laboratory Autoclavable Rodent Diet 5010, LabDiet, St. Louis, MO, USA), unless fasting for metabolic assays (discussed below). All animal work in this study was conducted with the approval of the Institutional Animal Care and Use Committee at the University of Pennsylvania (Protocol Number: 803545).