Chromolith reverse phase column rp 18 endcapped 100 2mm

Manufactured by Merck Group

The Chromolith reverse phase column (RP-18 endcapped 100–2mm) is a type of chromatography column used for the separation and analysis of chemical compounds. The column features a stationary phase that is composed of silica particles with a C18 alkyl coating, which enables the separation of analytes based on their hydrophobic interactions. This column is designed for use in high-performance liquid chromatography (HPLC) applications.

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Lab products found in correlation

Jetstream source, by Agilent Technologies (2 mentions) Hydroxylamine hydrochloride, by Merck Group (2 mentions) Masshunter software, by Agilent Technologies (2 mentions) Masshunter workstation software, by Agilent Technologies (2 mentions) 13c3 testosterone, by Cerilliant (1 mentions) Tert butyl methyl ether, by Merck Group (1 mentions) Prism version 8, by GraphPad (1 mentions) 6495 triple quadrupole mass spectrometer, by Agilent Technologies (1 mentions) Infinity 2 uhplc, by Agilent Technologies (1 mentions)

2 protocols using chromolith reverse phase column rp 18 endcapped 100 2mm

Quantifying Testosterone by UHPLC-MS/MS

Cited 2 times

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Testosterone quantification was determined using Agilent’s UHPLC Infinity II and 6495 triple quadrupole mass spectrometer, and MassHunter workstation software (8.0.8.23.5). Briefly, plasma samples containing 0.5 ng/mL ¹³C₃-testosterone (Cerilliant, Round Rock, TX) were extracted with tert-butyl methyl ether (Sigma 34875), dried, and derivatized using hydroxylamine hydrochloride (Sigma 431362). The recovered ketoxime steroids were reconstituted in methanol/water (1:1 v/v) and injected into the Infinity II UHPLC. Ketoxime steroids were separated using a Chromolith reverse phase column (RP-18 endcapped 100–2mm, Sigma 152006) and introduced into a JetStream source (Agilent) for triple quadrupole analysis. Data were analyzed and quantified using MassHunter software (Agilent) and GraphPad PRISM (version 8.0) software was used to graph and perform statistics (two-sided Students t-test)^{61 (link),62 (link)}.

Vellano C.P., White M.G., Andrews M.C., Chelvanambi M., Witt R.G., Daniele J.R., Titus M., McQuade J.L., Conforti F., Burton E.M., Lastrapes M.J., Ologun G., Cogdill A.P., Morad G., Prieto P., Lazar A.J., Chu Y., Han G., Khan M.A., Helmink B., Davies M.A., Amaria R.N., Kovacs J.J., Woodman S.E., Patel S., Hwu P., Peoples M., Lee J.E., Cooper Z.A., Zhu H., Gao G., Banerjee H., Lau M., Gershenwald J.E., Lucci A., Keung E.Z., Ross M.I., Pala L., Pagan E., Segura R.L., Liu Q., Borthwick M.S., Lau E., Yates M.S., Westin S.N., Wani K., Tetzlaff M.T., Haydu L.E., Mahendra M., Ma X., Logothetis C., Kulstad Z., Johnson S., Hudgens C.W., Feng N., Federico L., Long G.V., Futreal P.A., Arur S., Tawbi H.A., Moran A.E., Wang L., Heffernan T.P., Marszalek J.R, & Wargo J.A. (2022). Androgen receptor blockade promotes response to BRAF/MEK-targeted therapy. Nature, 606(7915), 797-803.

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Quantifying Testosterone and Corticosterone

Cited 2 times

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Testosterone and corticosterone quantification was determined using Agilent’s Infinity II UHPLC in line with a 6495 triple quadrupole mass spectrometer and MassHunter workstation software (8.0.8.23.5). Briefly, DSRCT xenograft and PDX samples were homogenized using water containing internal standard (Cerilliant, T070) extracted with tert-butyl methyl ether (Sigma 34875), dried under nitrogen, and derivatized using hydroxylamine hydrochloride (Sigma 431362). The recovered ketoxime steroids were reconstituted in methanol/water (1:1 v/v) and injected into the Infinity II UHPLC. Ketoxime steroids were separated using a Chromolith reverse-phase column (RP-18 endcapped 100–2 mm, Sigma 152006) and introduced into a JetStream source (Agilent) for triple quadrupole analysis. Data were analyzed and quantified using MassHunter software (Agilent)^{39 (link),40 (link)}.

Lamhamedi-Cherradi S.E., Maitituoheti M., Menegaz B.A., Krishnan S., Vetter A.M., Camacho P., Wu C.C., Beird H.C., Porter R.W., Ingram D.R., Ramamoorthy V., Mohiuddin S., McCall D., Truong D.D., Cuglievan B., Futreal P.A., Velasco A.R., Anvar N.E., Utama B., Titus M., Lazar A.J., Wang W.L., Rodriguez-Aguayo C., Ratan R., Livingston J.A., Rai K., MacLeod A.R., Daw N.C., Hayes-Jordan A, & Ludwig J.A. (2022). The androgen receptor is a therapeutic target in desmoplastic small round cell sarcoma. Nature Communications, 13, 3057.

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