Panther instrument

Manufactured by Hologic

Sourced in United States

The Panther instrument is a fully automated molecular diagnostic system designed for high-throughput laboratory testing. Its core function is to automate the processes of sample preparation, amplification, and detection of target analytes in clinical specimens.

Automatically generated - may contain errors

Lab products found in correlation

Cobas z 480 analyser, by Roche (1 mentions) Aptima sars cov 2 assay, by Hologic (1 mentions)

3 protocols using panther instrument

SARS-CoV-2 Serological Antibody Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Positivity for SARS-CoV-2 was diagnosed through nasopharyngeal swab samples by using a home-brew real-time polymerase chain reaction or a transcription-mediated amplification (TMA) assay on the Panther instrument (Hologic).
The total antibody against SARS-CoV-2 in serum samples was tested using an enzyme-linked immunosorbent assay kit supplied by Beijing (Wantai Biological Pharmacy Enterprise Co., Ltd.) according to the manufacturer’s instructions. Briefly, the enzyme-linked immunosorbent assay for total antibodies detection (IgG/IgM/IgA) was developed on the basis of double-antigen sandwich immunoassay by using mammalian cell–expressed recombinant antigens containing the receptor-binding domain of the spike protein of SARS-CoV-2 as the immobilized and horseradish peroxidase–conjugated antigen. Samples were considered as positive if the signal-to-cutoff (S/Co) was >1.

Del Bello A., Kamar N., Vergez F., Faguer S., Marion O., Beq A., Lathrache Y., Abravanel F., Izopet J, & Treiner E. (2021). Adaptive lymphocyte profile analysis discriminates mild and severe forms of COVID-19 after solid organ transplantation. Kidney International, 100(4), 915-927.

+ Open protocol

+ Expand

Evaluation of CT/NG Molecular Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Specimens collected for the cobas 4800 CT/NG Test, which targets chromosomal DNA in CT (ompA) and NG (2 DR-9 region targets) in addition to cryptic plasmid DNA in CT (12 (link)), were stored at room temperature and tested the next workday after specimen collection. Specimen preparation was performed on the cobas x 480 instrument (Roche) and amplification/detection on the cobas z 480 analyser (Roche), according to the manufacturer’s instructions. Specimens collected for the Aptima Combo 2 Assay, which targets 23S rRNA in CT and 16S rRNA in NG (13 (link)), were stored at –80°C for a maximum of 3 months until testing at the WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections at the Örebro University Hospital in Sweden.
Discrepant results between the cobas 4800 CT/NG and Aptima Combo 2 assays were resolved with the singleplex Aptima Chlamydia trachomatis and Aptima Neisseria gonorrhoeae assays (Hologic). In both CT and NG, these assays target species-specific regions in 16S rRNA; for NG the target region differs from the one used in the Aptima Combo 2 assay. All Aptima assays were performed by using the Aptima collection tubes on the Panther instrument (Hologic), according to the manufacturer’s instructions. Results were considered true positive for CT or NG if at least 2 of the 3 assays yielded positive results.

HILMARSDÓTTIR I., ARNARDÓTTIR E.M., JÓHANNESDÓTTIR E.R., GOLPARIAN D, & UNEMO M. (2021). Chlamydia trachomatis in Iceland: Prevalence, Clinico-epidemiological Features and Comparison of Cobas 480 CT/NG and Aptima Combo 2 (CT/NG) for Diagnosis. Acta Dermato-Venereologica, 101(2), 1040.

+ Open protocol

+ Expand

SARS-CoV-2 Detection Using Aptima Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Aptima SARS-CoV-2 assay on the Panther instrument (Hologic, Inc., San Diego, CA, USA) targets two parts of the ORF1 ab region of the SARS-CoV-2 genome and one internal control. This test is based on End-Point Transcription-Mediated Amplification (EP-TMA), which is a binary test for the presence or absence of SARS-CoV-2 [11 (link),12 (link)]. After amplification, chemiluminescent probes hybridize to amplicons and emit light measured by a luminometer in relative light units (RLUs). For valid samples, the RLU value will range between approximately 250 (internal control only) and 1250 RLUs with a positive internal control, and two positive targets (of 500 RLUs each). Assay results are determined by a cutoff based on the total RLU and the kinetic curve type.

Vahidnia A., Souverein D., Euser S.M., Haverkort M., Noordhuis E., van Zijl T.Z., Kalpoe J., Sinnige J.C, & Herpers B.L. (2023). Similar Sensitivity of SARS-CoV-2 Detection in Oropharyngeal/Nasopharyngeal and Saliva Samples on the Hologic Panther Platform. Diagnostics, 13(3), 347.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!