Mouse anti fancd2

Manufactured by Santa Cruz Biotechnology

The Mouse anti-FANCD2 is a primary antibody that specifically recognizes the FANCD2 protein in mouse samples. FANCD2 is a key component of the Fanconi anemia DNA repair pathway. This antibody can be used for applications such as Western blotting, immunoprecipitation, and immunohistochemistry to detect and study the FANCD2 protein.

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Lab products found in correlation

Bz x700, by Keyence (1 mentions) Mouse anti alpha tubulin, by Abcam (1 mentions) Rabbit anti p27, by Cell Signaling Technology (1 mentions) Mouse anti p53, by Santa Cruz Biotechnology (1 mentions) Rabbit anti 53bp1, by Abcam (1 mentions) Rabbit anti fancd2, by Abcam (1 mentions) Mouse anti vinculin, by Abcam (1 mentions) Rabbit anti fanca, by Fortis Life Sciences (1 mentions) Mouse anti mitf, by Abcam (1 mentions) Goat anti actin, by Abcam (1 mentions) Rabbit anti p21, by Santa Cruz Biotechnology (1 mentions) Mouse anti flag, by Merck Group (1 mentions)

2 protocols using mouse anti fancd2

Protein Interaction Analysis via PLA

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PLA analyses were performed according to the manufacturer’s instruction (Duolink, #DUO92101, Sigma). Primary antibodies were diluted as follows: rabbit anti-UBR5 (Cell Signaling Technology, #65344) at 1:100, mouse anti-XRCC3 (Santa Cruz, #sc-271714) at 1:50, and mouse anti-FANCD2 (Santa Cruz, #sc-20022) at 1:50. Imaging was performed using an All-in-one fluorescence microscope (BZ-X700, Keyence) under TexasRed and DAPI filters. The numbers of red PLA dots and cells from four to five random fields were quantified by ImageJ (v1.52p)^{[44 (link)]}.

Yan Y., Zhou B., Lee Y.J., You S., Freeman M.R, & Yang W. (2021). BoxCar and Shotgun Proteomic Analyses Reveal Molecular Networks Regulated by UBR5 in Prostate Cancer. Proteomics, 22(4), e2100172.

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Comprehensive Antibody Panel for Cell Analysis

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The following antibodies were used: mouse anti-MiTF (Abcam), mouse anti-FANCD2 (Santa Cruz), rabbit anti-FANCD2 (Abcam), rabbit anti-FANCA (Bethyl), mouse anti-Flag (Sigma), rabbit anti-p53 serine 15 (Cell Signaling Technology), mouse anti-p53 (Santa Cruz), rabbit anti p27 (Cell Signaling), rabbit anti-p21 (Santa Cruz), mouse anti-Cyclin-A (Abcam), rabbit anti-53BP1 (Abcam), mouse anti-gH2AX (Millipore), mouse anti-alpha-tubulin (Abcam), mouse anti-vinculin (Abcam), and goat anti-actin (Abcam).

Bourseguin J., Bonet C., Renaud E., Pandiani C., Boncompagni M., Giuliano S., Pawlikowska P., Karmous-Benailly H., Ballotti R., Rosselli F, & Bertolotto C. (2016). FANCD2 functions as a critical factor downstream of MiTF to maintain the proliferation and survival of melanoma cells. Scientific Reports, 6, 36539.

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