Sections stained with H&E were digitized using a Pannoramic MIDI scanner and viewed with CaseViewer software (3DHISTECH, Budapest, Hungary). Pseudocolor images of immunostained sections were acquired with a Leica SP8 confocal laser scanning microscope using Leica objectives (20×/0.7 dry HC PL Apo, 40×/1.3 oil HC PL Apo CS2, 63×/1.4 oil HC PL Apo or 100×/1.3 oil HC PL Fluotar), operating under Leica Application Suite X microscope software (LAS X, Leica Microsystems, Buffalo Grove, IL, USA). Obtained images were optimized using Huygens Deconvolution software (Scientific Volume Imaging, Hilversum, the Netherlands). Maximal projections were obtained from image stacks with optimized z‐step size. Brightness and contrast adjustments were performed with Fiji (ImageJ version 1.52p) or Adobe Photoshop CC 2018. For separate channels, the corresponding author can be contacted.
Application suite x microscope software
Manufactured by Leica
Sourced in United States
Leica Application Suite X (LAS X) is a comprehensive microscope software that provides a user-friendly interface for controlling Leica microscopes and imaging devices. The software enables image acquisition, analysis, and management, supporting a range of microscopy techniques and sample types.
Automatically generated - may contain errors
2 protocols using application suite x microscope software
1
Microscopic Imaging of Tissue Sections
2
Confocal Imaging of Explant Tissues
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Pseudocolor images of explant tissues were acquired with a Leica SP8 confocal laser scanning microscope using Leica objectives (20×/0.7 dry HC PL Apo, 40×/1.3 oil HC PL Apo CS2, 63×/1.4 oil HC PL Apo or 100×/1.3 oil HC PL Fluotar) and operated under Leica Application Suite X microscope software (LAS X, Leica Microsystems, Buffalo Grove, IL, USA). The laser intensity ranged from 1 to 4% for different channels, with a HyD gain of 10–25. Areas within the sample were randomly selected for acquisition. For cochlear samples, the presumed tonotopical locations of acquisition ranged between 20,000 Hz and 4000 Hz. Maximal projections were obtained from image stacks with optimized z-step size. Brightness and contrast adjustments were performed with Fiji (ImageJ version 1.52p) or Adobe Photoshop CC 2018.
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