At 2–3 weeks post cell implantation and immediately before the MRI measurements, mice were shaved at the site of implantation and the planar red fluorescence emission from transgenic cells imaged using an IVIS-200 camera (Perkin Elmer, Waltham, MA). The animals were anesthetized using 2% Isoflurane and 75 % air / 25 % O2 (flow rate 2 L min-1), and the images analyzed using Living Image software vs. 4.4 (Perkin Elmer). Images were acquired with epi-illumination, small binning, aperture range F2-F8, exposure 1-3s, and the field of view was varied between 13 and 19.4 cm. The excitation and emission pass band ranges were 500-550 nm and 575–650 nm, respectively. A red background pass band correction filter (460–490 nm) was used to suppress autofluorescence. Rats were anesthetized and bioluminescence imaged using the IVIS-200 camera at approximately 5 minutes after luciferin injection (1 ml of 15mg/ml solution i.p.).
Living image software vs 4
Manufactured by PerkinElmer
Living Image software is a visualization and analysis platform developed by PerkinElmer for in vivo imaging applications. It provides tools for the acquisition, processing, and analysis of data from various imaging modalities, including optical, PET, and SPECT. The software's core function is to enable researchers to quantify and interpret biological processes in living subjects.
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Lab products found in correlation
2 protocols using living image software vs 4
1
In Vivo Fluorescence and Bioluminescence Imaging
2
In Vivo Fluorescence and Bioluminescence Imaging
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At 2–3 weeks post cell implantation and immediately before the MRI measurements, mice were shaved at the site of implantation and the planar red fluorescence emission from transgenic cells imaged using an IVIS-200 camera (Perkin Elmer, Waltham, MA). The animals were anesthetized using 2% Isoflurane and 75 % air / 25 % O2 (flow rate 2 L min-1), and the images analyzed using Living Image software vs. 4.4 (Perkin Elmer). Images were acquired with epi-illumination, small binning, aperture range F2-F8, exposure 1-3s, and the field of view was varied between 13 and 19.4 cm. The excitation and emission pass band ranges were 500-550 nm and 575–650 nm, respectively. A red background pass band correction filter (460–490 nm) was used to suppress autofluorescence. Rats were anesthetized and bioluminescence imaged using the IVIS-200 camera at approximately 5 minutes after luciferin injection (1 ml of 15mg/ml solution i.p.).
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