Ocean optics maya2000 pro

Spectral characterization was performed using a xenon light source (Thorlabs SLS401; Thorlabs GmbH, Dachau, Germany) and a ZEISS Axio Scope.A1 microscope (Zeiss AG, Oberkochen, Germany). The light reflected from the sample was collected by an optical fiber (230 µm core) in a plane confocal to the image plane, resulting in an effective measurement spot diameter of ≈13 µm at a magnification of × 20. The spectra were analyzed by a spectrometer (Ocean Optics Maya2000 Pro; Ocean Optics, Dunedin, FL, USA), a white Lambertian diffuser served as a reference. Optical micrographs were captured with a CCD camera (GS3‐U3‐28S5C‐C, FLIR Integrated Imaging Solutions Inc., Richmond, Canada). Reflection spectra were taken on scales still attached to the elytron. Transmission spectra were also taken with the same equipment, but the scales were first detached from the elytron and deposited on a microscope glass slide using a scalpel, before immersion in a refractive index matching oil (n_o = 1.55). Scatterometry measurements were performed by placing a Bertrand lens (Zeiss 453671) into the imaging pathway and using a high numerical aperture air objective (Zeiss Epiplan Neofluar 100×, NA 0.9). This allowed measurements of scattering angles up to 64°.

Fluorescent properties of fluorescein-maleimide-labelled nanocomposites were analyzed using an OceanOptics Maya2000 Pro (Ocean Insight, Orlando, FL, USA) fiber optic spectrophotometer with a quasi-monochromatic LED, λmax = 365 nm (HAMAMATSU, LC1) and a long-pass filter (cut on 400 nm). Samples were prepared by placing 2.5 mL of the appropriate solution/dispersion into 10 mm Hellma™ Suprasil™ cuvettes.