Anti trxr1

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-TrxR1 is a laboratory reagent that can be used to detect and quantify the expression of Thioredoxin Reductase 1 (TrxR1) in various biological samples. TrxR1 is an enzyme that plays a crucial role in cellular redox homeostasis and is involved in various physiological and pathological processes. The Anti-TrxR1 reagent can be used in techniques such as western blotting, immunohistochemistry, and ELISA to measure the levels of TrxR1 in cells, tissues, or other biological samples.

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6 protocols using anti trxr1

Gastric Cancer Cell Apoptosis Assay

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EF24, N-acetylcysteine (NAC), catalase and rhTrxR1 protein were purchased from Sigma (St. Louis, MO). Erastin was purchased from Selleck Chemicals. Human gastric cancer cell lines SGC-7901, BGC-823 and KATO III, normal Human Gastric Epithelial Cell Line (GES-1) and normal Rat Kidney Proximal Cell Line (NRK-52E) were purchased from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. The cells were routinely cultured in RPMI 1640 medium (Gibco, Eggenstein, Germany) containing 10% heat-inactivated fetal bovine serum (Gibco, Eggenstein, Germany), 100 units/mL penicillin, and 100 μg/mL streptomycin in a humidified cell incubator with an atmosphere of 5% CO₂ at 37°C. Antibodies including anti-Bcl-2, anti-Bax, anti-cleaved PARP, anti-TrxR1, anti-GAPDH, goat anti-mouse IgG-HRP and donkey anti-rabbit IgG-HRP were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies including anti-CHOP, anti-ATF4, anti-p-eIF2α and anti-eIF2α were purchased from Cell Signaling Technology (Danvers, MA). FITC Annexin V apoptosis Detection Kit I and Propidium Iodide (PI) were purchased from BD Pharmingen (Franklin Lakes, NJ).

Zou P., Xia Y., Chen W., Chen X., Ying S., Feng Z., Chen T., Ye Q., Wang Z., Qiu C., Yang S, & Liang G. (2016). EF24 induces ROS-mediated apoptosis via targeting thioredoxin reductase 1 in gastric cancer cells. Oncotarget, 7(14), 18050-18064.

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Cytotoxicity Evaluation of PL Compound

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PL (S7551) was purchased from Selleck Chemical (Shanghai, China), the purity of PL is 99.33%. N-Acetylcysteine (NAC) was purchased from Sigma-Aldrich (St. Louis, MO, USA). PI was purchased from BD Pharmingen (Franklin Lakes, NJ). Hoechst stain and DCFH-DA were purchased from Beyotime Biotechnology (Nantong, China). Anti-Cdc2, anti-Bcl-2, anti-Bax, anti-CyclinB1, anti-TrxR1, and anti-Ki67 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA), and anti-ATF-4, anti-EIF2α, anti-CHOP, and anticleaved caspase-3 antibodies were purchased from Cell Signaling Technology (Danvers, MA). HRP-conjugated secondary antibodies were also obtained from Cell Signaling Technology.

Zhang Q., Chen W., Lv X., Weng Q., Chen M., Cui R., Liang G, & Ji J. (2019). Piperlongumine, a Novel TrxR1 Inhibitor, Induces Apoptosis in Hepatocellular Carcinoma Cells by ROS-Mediated ER Stress. Frontiers in Pharmacology, 10, 1180.

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Characterizing Apoptosis and Oxidative Stress in Gastric Cancer Cells

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Human gastric cancer cell lines SGC-7901, BGC- 823 and KATO III were purchased from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. The cells were routinely cultured in RPMI 1640 medium (Gibco, Eggenstein, Germany) containing 10% heat-inactivated fetal bovine serum (Hyclone, Logan, UT), 100 units/ml penicillin, and 100 ug/ml streptomycin in a humidified cell incubator with an atmosphere of 5% CO2 at 37°C. FITC Annexin V apoptosis Detection Kit I and propidium iodide (PI) were purchased from BD Pharmingen (Franklin Lakes, NJ). 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) and JC-1 were purchased from Invitrogen (Carlsbad, CA). Antibodies including anti-Cdc2, anti-XBP-1, a'nti-Bcl-2, anti-Bax, anti-Cyclin B1, anti-cleaved PARP, anti-MDM-2, anti-p53, anti-TrxR1, anti-Ki67, anti-GAPDH, goat anti-mouse IgG-HRP, donkey anti-rabbit IgG-HRP and donkey anti-goat IgG-HRP were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies including anti-ATF-4, anti-p-EIF2α, anti-CHOP, anti-Cle-caspase3, were purchased from Cell Signaling Technology (Danvers, MA).

Chen W., Zou P., Zhao Z., Weng Q., Chen X., Ying S., Ye Q., Wang Z., Ji J, & Liang G. (2016). Selective killing of gastric cancer cells by a small molecule via targeting TrxR1 and ROS-mediated ER stress activation. Oncotarget, 7(13), 16593-16609.

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Antioxidant Protein Expression Analysis

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Expression levels of various antioxidant proteins were evaluated via western blotting. Briefly, 5 × 10⁶ cells in 100 mm culture dishes (BD Falcon) were incubated with the indicated concentrations of Tempol (0.5–4 mM) for 48 h. Cells were washed with PBS and four volumes of lysis buffer (Intron Biotechnology, Seongnam, Gyeonggi-do, Korea) were added. Total proteins (30 μg) were resolved in 12.5% SDS-PAGE gels and then transferred to Immobilon-P PVDF membranes (Millipore, Billerica, MA, USA) by electroblotting. Membranes were probed with anti-SOD1, anti-catalase, anti-Trx1, anti-TrxR1, and anti-β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Santa Cruz Biotechnology). Western blots were developed using an EZ-Western Lumi Pico ECL solution kit (DoGen, Seoul, Korea). All band intensities were analyzed using the ImageJ software program (FujiFilm, Tokyo, Japan).

, & Park W.H. (2021). Tempol differently affects cellular redox changes and antioxidant enzymes in various lung-related cells. Scientific Reports, 11, 14869.

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Antibody Panel for Cell Cycle and Oxidative Stress Analysis

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Anti-glutathione peroxidase 1/2 (catalog no. sc-133160), anti-glutathione peroxidase 4 (catalog no. sc-166570), anti-superoxide dismutase 2 (catalog no. sc-137254), anti-TrxR1 (catalog no. sc-28321), and anti-heme oxygenase 1 (catalog no. sc-136960) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-cyclinD1 (catalog no. 2978), anti-cyclinD3 (catalog no. 2936), anti-CDK6 (catalog no. 3136), anti-CDK4 (catalog no. 2906), anti-CDKN1A (catalog no. 2946), anti-CDKN2C (catalog no. 2896), anti-CDKN2B (catalog no. 4822), anti-beta1 integrin (CD29) (catalog no. 4706), and anti-RUNX2 (catalog no. 12556) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-β-Actin antibody (catalog no. A5441) was obtained from Sigma-Aldrich (Saint Louis, MO, USA). Anti-VCAM1 antibody (catalog no. 66294-1-Ig) was obtained from Proteintech group (Rosemont, IL, USA). Anti-CD34 antibody (catalog no. 81289) was purchased from Abcam (Cambridge, MA, USA). Osteoblast-Inducer Reagent (catalog no. MK430) was obtained from Takara Bio. Inc. (Shiga, Japan).

Kanno K., Sakaue T., Hamaguchi M., Namiguchi K., Nanba D., Aono J., Kurata M., Masumoto J., Higashiyama S, & Izutani H. (2021). Hypoxic Culture Maintains Cell Growth of the Primary Human Valve Interstitial Cells with Stemness. International Journal of Molecular Sciences, 22(19), 10534.

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Antibody Panel for EMT Markers

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Anti-Snail, anti–E-Cadherin, anti–N-Cadherin, anti–Slug, anti–peroxiredoxin(Prx), anti-thioredoxin(Trx1), anti-phosphotyrosine(Y416)-Src, anti–phospho-Erk1/2, anti-phosphotyrosine-Jak1, anti-phosphotyrosine-Stat1, and anti-tubulin Abs were from Cell Signaling Technology (Beverley, MA). Anti-SOCS1, anti-vimentin1, anti-TrxR1, and anti–SOD1 Abs were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-p65 Ab was from Upstate Biochemicals (Charlottesville, VA). Anti-LaminB1 and anti-c-Src Abs were from Abcam (Cambridge, MA).

Jung S.H., Kim S.M, & Lee C.E. (2016). Mechanism of suppressors of cytokine signaling 1 inhibition of epithelial-mesenchymal transition signaling through ROS regulation in colon cancer cells: suppression of Src leading to thioredoxin up-regulation. Oncotarget, 7(38), 62559-62571.

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