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Jnm la 300

Manufactured by Bruker
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The JNM-LA 300 is a nuclear magnetic resonance (NMR) spectrometer designed for laboratory use. It is capable of analyzing the structure and properties of chemical compounds through the detection and analysis of nuclear magnetic resonances.

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Lab products found in correlation

Silica gel 60, by Merck Group (6 mentions) Avance 400 mhz ft nmr, by Bruker (4 mentions) Eclipse plus c18 column, by Daicel (4 mentions) Ft ir 4200 spectrometer, by Jasco (2 mentions) Od h column, by Daicel (2 mentions) Avance 400 mhz ft nmr spectrometer, by Bruker (2 mentions) 1120 compact lc, by Agilent Technologies (2 mentions) Chiralcel od h column, by Daicel (2 mentions) Tc c18 column, by Agilent Technologies (1 mentions)

7 protocols using jnm la 300

1

Synthesis and Characterization of Organic Compounds

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All chemical reagents were commercially available. Melting points were determined on a Büchi Melting Point B-540 apparatus and are uncorrected. Silica gel column chromatography was performed on silica gel 60, 230–400 mesh, Merck. Nuclear magnetic resonance (1H NMR and 13C NMR) spectra were recorded on JEOL JNM-LA 300 [300 MHz (1H), 75 MHz (13C)] and Bruker Avance 400 MHz FT-NMR [400 MHz (1H), 100 MHz (13C)] spectrometers. Chemical shifts are reported in ppm units with Me4Si as a reference standard. Infrared (IR) spectra were recorded on a JASCO FT/IR-4200 spectrometer. Mass spectra were recorded on a VG Trio-2 GC-MS and 6460 Triple Quad LC/MS. All final compounds were purified to >95% purity, as determined by high-performance liquid chromatography (HPLC). HPLC was performed on an Agilent 1120 Compact LC (G4288A) instrument using an Agilent Eclipse Plus C18 column (4.6 × 250 mm, 5 μm) and a Daicel Chiralcel OD-H column (4.6 × 250 mm, 5 μm).
Ann J., Jung A., Kim M.Y., Kim H.M., Ryu H., Kim S., Kang D.W., Hong S., Cui M., Choi S., Blumberg P.M., Frank-Foltyn R., Bahrenberg G., Stockhausen H., Christoph T, & Lee J. (2015). Structure activity relationships of benzyl C-region analogs of 2-(3-fluoro-4-methylsulfonamidophenyl)propanamides as potent TRPV1 antagonists. Bioorganic & medicinal chemistry, 23(21), 6844-6854.
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2

Analytical Techniques for Organic Synthesis

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All chemical reagents were commercially available. Silica gel column chromatography was performed on silica gel 60, 230–400 mesh, Merck. Nuclear magnetic resonance (1H NMR) spectra were recorded on a JEOL JNM-LA 300 and a Bruker Avance 400 MHz FT-NMR spectrometer. Chemical shifts are reported in ppm units with Me4Si as a reference standard. Mass spectra were recorded on a VG Trio-2 GC-MS and a 6460 Triple Quad LC/MS. Elemental analyses were performed with an EA 1110 Automatic Elemental Analyzer, CE Instruments.
Ann J., Yoon S., Baek J., Kim D.H., Lewin N.E., Hill C.S., Blumberg P.M, & Lee J. (2014). Design and synthesis of protein kinase C epsilon selective diacylglycerol lactones (DAG-lactones). European journal of medicinal chemistry, 90, 332-341.
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3

Synthesis and Characterization of Novel Compounds

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All chemical reagents were commercially available. Melting points were determined on a Büchi Melting Point B-540 apparatus and are uncorrected. Silica gel column chromatography was performed on silica gel 60, 230–400 mesh, Merck. Nuclear magnetic resonance (1H NMR and 13C NMR) spectra were recorded on JEOL JNM-LA 300 [300 MHz (1H), 75 MHz (13C)] and Bruker Avance 400 MHz FT-NMR [400 MHz (1H), 100 MHz (13C)] spectrometers. Chemical shifts are reported in ppm units with Me4Si as a reference standard. Mass spectra were recorded on a VG Trio-2 GC–MS and 6460 Triple Quad LC/MS. All final compounds were purified to >95% purity, as determined by high-performance liquid chromatography (HPLC). HPLC was performed on an Agilent 1120 Compact LC (G4288A) instrument using an Agilent Eclipse Plus C18 column (4.6 × 250 mm, 5 μm) and a Daicel Chiralcel OD-H column (4.6 × 250 mm, 5 μm).
Ann J., Ki Y., Yoon S., Kim M.S., Lee J.U., Kim C., Lee S., Jung A., Baek J., Hong S., Choi S., Pearce L.V., Esch T.E., Turcios N.A., Lewin N.E., Ogunjirin A.E., Herold B.K., McCall A.K., Blumberg P.M, & Lee J. (2016). 2-Sulfonamidopyridine C-region analogs of 2-(3-fluoro-4-methylsulfonamidophenyl)propanamides as potent TRPV1 antagonists. Bioorganic & medicinal chemistry, 24(6), 1231-1240.
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4

Synthesis and Characterization of Organic Compounds

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All chemical reagents were commercially available. Melting points were determined on a Büchi Melting Point B-540 apparatus and are uncorrected. Silica gel column chromatography was performed on silica gel 60, 230–400 mesh, Merck. Nuclear magnetic resonance (1H NMR and 13C NMR) spectra were recorded on JEOL JNM-LA 300 [300 MHz (1H), 75 MHz (13C)] and Bruker Avance 400 MHz FT-NMR [400 MHz (1H), 100 MHz (13C)] spectrometers. Chemical shifts are reported in ppm units with Me4Si as a reference standard. Infrared (IR) spectra were recorded on a JASCO FT/IR-4200 spectrometer. Mass spectra were recorded on a VG Trio-2 GC-MS and 6460 Triple Quad LC/MS. All final compounds were purified to >95% purity, as determined by high-performance liquid chromatography (HPLC). HPLC was performed on an Agilent 1120 Compact LC (G4288A) instrument using an Agilent Eclipse Plus C18 column (4.6 × 250 mm, 5 μm) and a Daicel Chiralcel OD-H column (4.6 × 250 mm, 5 μm).
Ryu H., Seo S., Lee J.Y., Ha T.H., Lee S., Jung A., Ann J., Kim S.E., Yoon S., Hong M., Blumberg P.M., Frank-Foltyn R., Bahrenberg G., Schiene K., Stockhausen H., Christoph T., Frormann S, & Lee J. (2015). Pyridine C-region analogs of 2-(3-fluoro-4-methylsulfonylaminophenyl)propanamides as potent TRPV1 antagonists. European journal of medicinal chemistry, 93, 101-108.
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5

Silica Gel Column Chromatography for Organic Synthesis

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All chemical reagents were commercially available. Silica gel column chromatography was performed on silica gel 60, 230-400 mesh, Merck. Nuclear magnetic resonance (1H NMR) spectra were recorded on a JEOL JNM-LA 300 and a Bruker Avance 400 MHz FTNMR spectrometer. Chemical shifts are reported in ppm units with Me4Si as a reference standard. Mass spectra were recorded on a VG Trio-2 GC-MS and a 6460 Triple Quad LC/MS.
Lee H., Ahn S., Ann J., Ha H., Yoo Y.D., Kim Y.H., Hwang J.Y., Hur K.H., Jang C.G., Pearce L.V., Esch T.E., Lewin N.E., Blumberg P.M, & Lee J. (2019). Discovery of Dual-acting Opioid Ligand and TRPV1 Antagonists as Novel Therapeutic Agents for Pain. European journal of medicinal chemistry, 182, 111634.
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6

Synthesis and Purification of Compounds

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All chemical reagents were commercially available and used directly without any purification. Silica gel column chromatography was performed on silica gel 60 of 230–400 mesh from Merck. 1H NMR spectra were recorded on a JEOL JNM-LA 300 at 300 MHz by Bruker Analytik or on a DE/AVANCE Digital 400 at 400 MHz by Bruker Analytik, with Me4Si as a reference standard. Mass spectra were recorded on a VG Trio-2 GC-MS instrument and a 6460 Triple Quad LC-MS instrument. All final compounds were assessed for purity by high performance liquid chromatography (HPLC) on Agilent 1120 Compact LC (G4288A) system via the following conditions. Column: Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm). Mobile phase A: 0.1% TFA in MeOH, mobile phase B: 0.1% TFA in water (v/v) in 30 min. Wavelength: 254 nM. Flow: 0.7 to 1.0 mL min−1. According to the HPLC analyses, all final compounds showed a purity of ≥95%. In addition, detailed information of inhibitors was described in the supplementary (ESI file†).
Tran P.T., Hoang V.H., Lee J., Hien T.T., Tung N.T, & Ngo S.T. (2019). In vitro and in silico determination of glutaminyl cyclase inhibitors. RSC Advances, 9(51), 29619-29627.
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7

Analytical Characterization of Organic Compounds

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All chemical reagents were commercially available. Silica gel column chromatography was performed on silica gel 60, 230–400 mesh, Merck. Nuclear magnetic resonance (1H NMR and 13C NMR) spectra were recorded on JEOL JNM-LA 300 [300 MHz (1H), 75 MHz (13C)] and Bruker Avance 400 MHz FT-NMR [400 MHz (1H), 100 MHz (13C)] spectrometers. Chemical shifts are reported in ppm units with Me4Si as a reference standard. Mass spectra were recorded on a VG Trio-2 GC-MS and 6460 Triple Quad LC/MS. All final compounds were purified to >95% purity, as determined by high-performance liquid chromatography (HPLC). HPLC was performed on an Agilent 1120 Compact LC (G4288A) instrument using an Agilent Eclipse Plus C18 column (4.6 × 250 mm, 5 μm) and a Daicel Chiralcel OD-H column (4.6 × 250 mm, 5 μm).
Lee S., Kang D.W., Ryu H., Kim C., Ann J., Lee H., Kim E., Hong S., Choi S., Blumberg P.M., Frank-Foltyn R., Bahrenberg G., Stockhausen H., Christoph T, & Lee J. (2017). t-Butyl pyridine and phenyl C-region analogues of 2-(3-fluoro-4-methylsulfonylaminophenyl)propanamides as potent TRPV1 antagonists. Bioorganic & medicinal chemistry, 25(8), 2451-2462.
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