Vs asw l100

Manufactured by Olympus

Sourced in Germany

The VS-ASW-L100 is a laboratory equipment designed for sample analysis and preparation. It is a compact and versatile device that can be used for a variety of applications. The core function of the VS-ASW-L100 is to provide reliable and consistent results for sample processing and data collection.

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Lab products found in correlation

Prolong glass mounting medium without dapi, by Thermo Fisher Scientific (2 mentions) Orca r2 model c10600, by Hamamatsu Photonics (2 mentions) Lsm 710, by Zeiss (2 mentions) Lsm 700, by Zeiss (2 mentions) Vs120 slide scanner, by Olympus (2 mentions) Zen 2012 blue edition software, by Zeiss (1 mentions) Vs120, by Olympus (1 mentions) Ringer s lactate solution, by Fresenius (1 mentions) Hematoxylin eosin, by Merck Group (1 mentions) Masson s trichrome, by Merck Group (1 mentions) Goat serum, by Vector Laboratories (1 mentions) Goat anti mouse igg2a, by Thermo Fisher Scientific (1 mentions) Hfl 1, by Thermo Fisher Scientific (1 mentions) Dapi containing mounting medium, by Agilent Technologies (1 mentions)

6 protocols using vs asw l100

Widefield Fluorescence Imaging of Tissue Slices

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Slices were mounted on a glass slide with ProLong Glass mounting medium without DAPI (ThermoFisher Scientific) and imaged on a widefield slidescanner (Olympus VS-ASW-L100) using a 40X air objective (Olympus, 0.95 NA) and a 16-bit camera (Hamamatsu Orca-R² model C10600) with 7–11 focal planes (3μm per Z plane) per channel and automatic tiling. Focusing was done manually for ~100 focal reference points throughout each slice. The fluorescence filter sets used were FITC (Chroma #39002), ET-CY3/TRITC (Chroma # 49004) and Cy5 (Chroma # 39007).

Osorno T., Rudolph S., Nguyen T., Kozareva V., Nadaf N., Norton A., Macosko E.Z., Lee W.C, & Regehr W.G. (2022). Candelabrum cells are ubiquitous cerebellar cortex interneurons with specialized circuit properties. Nature neuroscience, 25(6), 702-713.

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Widefield Fluorescence Imaging of Tissue Slices

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Confocal Microscopy for Cellular Imaging and Analysis

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For imaging, confocal laser scanning microscopes LSM700 and LSM710 (Zeiss, Oberkochen, Germany) were used and gratefully provided by the microscopy core facility of SCI-TReCS (Spinal Cord Injury and Tissue Regeneration Center Salzburg). Images were taken as confocal z-stacks using 20 ×, 40 × and 63 × oil magnifications with 0.5, 0.6 or 1.0 zoom and combined to merged maximum intensity projections with the ZEN 2011 SP3 or SP7 (black edition) software (Zeiss, Oberkochen, Germany). For qualitative analysis, 2–3 animals per group were immunohistologically stained and analyzed. For quantitative analysis, 6 animals per group were stained and analyzed. Editing and processing of all images were performed using ZEN 2012 (blue edition) software (version 1.1.2.0 Zeiss, Oberkochen, Germany) and Microsoft PowerPoint. Three-dimensional rendering was performed using Imaris Software (version 9.1.2, Bitplane, https://imaris.oxinst.com/ (accessed on 10 January 2021)). Images of CD8+ T-cell stainings were taken with a virtual slide microscope, VS120, with the Olympus VS-ASW.L100 software (both from Olympus, https://www.olympus.de/ (accessed on 10 January 2021), Hamburg, Germany). Images were taken of whole sagittal sections as confocal z-stacks using 20× magnification.

Michael J., Zirknitzer J., Unger M.S., Poupardin R., Rieß T., Paiement N., Zerbe H., Hutter-Paier B., Reitsamer H, & Aigner L. (2021). The Leukotriene Receptor Antagonist Montelukast Attenuates Neuroinflammation and Affects Cognition in Transgenic 5xFAD Mice. International Journal of Molecular Sciences, 22(5), 2782.

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Biodistribution of Stromal Cells

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Animal experiments were performed in accordance with the guidelines of the “Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes” and were approved by the national animal care authorities (authorization number: BMWFW-66.019/0024-WF/V/36/2016). Stromal cells from three independent BM and UC donors were expanded for one passage and pooled to limit donor variation. TF-deficient BMSC were sorted to purity as described above immediately before tail vein injection into Fischer rats (1.5 million BMSC per 150 µL Ringer's lactate solution (Fresenius-Kabi, Austria) per injection; n = 3). For comparison, equal numbers of UC stromal cells previously checked for homogenous high TF expression were injected in three additional animals. Rats were sacrificed exactly one hour after stromal cell injection by CO₂ and lung, liver and spleen were surgically removed without perfusion before perfusion in 4% paraformaldehyde. Fixed organs were sectioned (4 µm) and processed for hematoxylin/eosin (Sigma-Aldrich, USA /Merck Milipore, Germany) and Masson's trichrome (MORPHISTO, Germany) histochemistry following standard protocols. Pictures were captured using the Olympus slidescanner VS120 at 40x magnification and processed using the Olympus VS-ASW-L100 software.

Oeller M., Laner-Plamberger S., Hochmann S., Ketterl N., Feichtner M., Brachtl G., Hochreiter A., Scharler C., Bieler L., Romanelli P., Couillard-Despres S., Russe E., Schallmoser K, & Strunk D. (2018). Selection of Tissue Factor-Deficient Cell Transplants as a Novel Strategy for Improving Hemocompatibility of Human Bone Marrow Stromal Cells. Theranostics, 8(5), 1421-1434.

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Quantifying Collagen Content in Tissue Sections

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Tissue sections were stained with Masson's trichrome (HT15, Sigma‐Aldrich) according to manufacturer's protocol, dehydrated, and mounted in pertex. Whole tissue sections were scanned (20 × magnification) using Olympus (Hamburg, Germany) VS120 slide scanner with XV image processer L100 VS‐ASW. With Visiopharm (VIS 6.1.0, Hoersholm, Denmark), parenchymal tissue were manually determined and analyzed for collagen content. Larger airways, vessels, and pleura were excluded from regions of interest. Positive‐stained area for collagen (blue staining) were quantified and related to total tissue area (excluding airspaces). Results were given as positive‐labeled area of collagen versus total tissue area (%). Image viewer software VS‐OlyVIA (version 2.9) (Olympus Soft Imaging solutions GmbH; Münster, Germany) was used for image visualization.

Löfdahl A., Rydell‐Törmänen K., Müller C., Martina Holst C., Thiman L., Ekström G., Wenglén C., Larsson‐Callerfelt A, & Westergren‐Thorsson G. (2016). 5‐HT 2B receptor antagonists attenuate myofibroblast differentiation and subsequent fibrotic responses in vitro and in vivo. Physiological Reports, 4(15), e12873.

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Immunofluorescence Analysis of Lung Fibroblasts

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Human fetal lung fibroblasts (HFL-1; ATCC, Rockville, USA) were used between passages 17 and 20. HFL-1 (10000 cells/well) and co-cultures of HFL-1 (10,000 cells/well) and LAD2 cells (7500 cells/well), were seeded in 4-well glass chamber slides (154526; Thermo Scientific, Waltham, MA) and incubated overnight at 37 °C, 5% CO₂. The cells were then fixed in 4% paraformaldehyde for 15 min and blocked in 2% BSA-TBS containing 5% goat serum (Vector laboratories, Burlingame, CA) and 0.2% Triton-X for 30 min, followed by washing twice in tris-buffered saline (TBS). Cells were incubated for 60 min with monoclonal tryptase antibody (M7052, Dako, Glostrup, Denmark) and monoclonal PAR2 antibody (cat.nr: 35–2300, Thermo Fisher Scientific, Waltham, Massachusetts, USA). The cells were then washed in TBS and incubated for 45 min with secondary antibodies (Thermo Fisher Scientific), goat anti-mouse IgG2a (Alexa Fluor® 647, A21241), goat anti-mouse IgG1 (Alexa Fluor® 647, A21240) or goat anti-mouse IgG1 (Alexa Fluor® 555, A21127), followed by washing in TBS. Nuclei were stained by using DAPI containing mounting medium (Dako). Cells were imaged using a VS120 slide scanner with XV image processor L100 VS-ASW (Olympus, Tokyo, Japan). Image viewer software VS-OlyVIA (version 2.9) (Olympus Soft Imaging solutions GmbH; Münster, Germany) was used for image visualization.

Bagher M., Larsson-Callerfelt A.K., Rosmark O., Hallgren O., Bjermer L, & Westergren-Thorsson G. (2018). Mast cells and mast cell tryptase enhance migration of human lung fibroblasts through protease-activated receptor 2. Cell Communication and Signaling : CCS, 16, 59.

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