Briefly, 0.125 g of tiger nut by-product powder was dissolved in a 10 mL solution of ethanol/water 40/60, v/v. The mixture was placed in an ultrasonic bath for 50 min, and then it was centrifuged for 10 min at 9000 rpm. The supernatant was collected, evaporated, and reconstituted in 1 mL of methanol/water (50:50, v/v). The final extracts were filtered with regenerated cellulose filters 0.2 μm (Millipore, Bedford, MA, USA) and stored at −18 °C until the analyses.
Regenerated cellulose filters 0.2 μm
Manufactured by Merck Group
Sourced in United States
Regenerated cellulose filters 0.2 μm are a type of laboratory filtration equipment designed to remove particles and contaminants from liquids. These filters have a pore size of 0.2 micrometers, which allows for the effective removal of small particles, bacteria, and other microscopic materials from the filtered solution.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using regenerated cellulose filters 0.2 μm
1
Extraction of Bioactive Compounds from Tiger Nut By-Product
2
Extraction of Bioactive Compounds from Orange By-Product
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Briefly, 1 g of orange by-product powder was added to 15 mL solution of ethanol/water 80:20 v/v. The mixture was placed in an ultrasonic bath for 15 min and then it was centrifuged for 10 min at 9000 rpm. The supernatant was collected, and the extraction was repeated twice more. Finally, the supernatants were evaporated, and reconstituted in 1 mL of methanol/water (50:50, v/v). The final extracts were filtered with regenerated cellulose filters 0.2 μm (Millipore, Bedford, MA, USA) and stored at −18 °C until the analyses.
3
Extraction of Avocado Polar Compounds
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A solid-liquid extraction was used to obtain the polar fraction of avocado pulp, peel and seed following a protocol from the literature with slight modifications (García-Salas, Gómez-Caravaca, Morales-Soto, Segura-Carretero, & Fernández-Gutiérrez, 2015) . Briefly, 2 g of freeze-dried sample powder were extracted with 20 mL hexane to eliminate lipids and then dissolved in 15 mL of a solution of methanol/ water (80:20, v/v). The mixture was placed in an ultrasonic bath for 15 min at room temperature and then centrifuged for 15 min at 1000 g. The supernatant was removed, and the extraction was repeated twice more. The supernatants were collected, evaporated, and reconstituted in 2 mL of methanol/water (80:20, v/v). The final extracts were filtered with regenerated cellulose filters 0.2 μm (Millipore, Bedford, MA, USA) and stored at -18 °C until analyzed.
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