Mouse igg 7076

Manufactured by Cell Signaling Technology

Sourced in United States

Mouse IgG (7076) is a laboratory reagent used as a detection and control antibody in various immunoassay and immunochemistry applications. It binds to mouse immunoglobulin G (IgG) molecules.

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Lab products found in correlation

P ikkα β, by Cell Signaling Technology (2 mentions) Rabbit igg 7074, by Cell Signaling Technology (2 mentions) Fatty acid free bovine serum albumin, by Equitech-Bio (1 mentions) Ab203070, by Abcam (1 mentions) Anti β actin a5441, by Merck Group (1 mentions) On targetplus non targeting pool sirna, by Horizon Discovery (1 mentions) Anti ctgf sc 14939, by Santa Cruz Biotechnology (1 mentions) Anti goat igg sc 2354, by Santa Cruz Biotechnology (1 mentions) β actin sc 69879, by Santa Cruz Biotechnology (1 mentions) Gapdh sc 32233, by Santa Cruz Biotechnology (1 mentions) Cdca5 ab192237, by Abcam (1 mentions) P iκbα, by Cell Signaling Technology (1 mentions) Lipopolysaccharide lps from escherichia coli o55 b5, by Merck Group (1 mentions) Mg132, by Beyotime (1 mentions)

4 protocols using mouse igg 7076

Handelin Extraction and Characterization

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Handelin (C₃₂H₄₀O₈; molecular weight 552.6552) was obtained from Baoji Herbest Bio-Tech Co., Ltd. (Shanxi, China) and affirmed by H¹NMR and MS data. The purity was determined to be > 98% by HPLC method. Fatty acid-free bovine serum albumin (BSA) was from Equitech-Bio, Inc. (Kerrville, TX, USA). Palmitic acid (PA, TCI, Shanghai, China) was dissolved in absolute ethanol as stock solution and then conjugated with 10% BSA to achieve a concentration of 100 μM as previously described (Li et al., 2015 (link)). The recombinant human Hsp70 protein was from Sino Biological Inc. (Beijing, China).
Antibodies against iNOS (2982), COX2 (12282), GAPDH (3683), HSP70 (4873), HSC70 (8444), K63-Ubi (12930), K48-Ubi (8081), p-IKKα/β (2697), IKKα (11930), IKKβ (8943), p-IκB-α (2859), IκB-α (4814), p-NF-κB p65 (3033), NF-κB p65 (8242), HA-Tag (3724), His-Tag (2366), TRAF6 (ab94720), IL-1β (12242), TNF-α (11948), rabbit IgG (7074) and mouse IgG (7076) were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against Iba-1 (ab178680), CD11b/c (ab202907) and CD68 (ab31630) were obtained from Abcam Biotechnology (Cambridge, UK).

Wang L.C., Liao L.X., Lv H.N., Liu D., Dong W., Zhu J., Chen J.F., Shi M.L., Fu G., Song X.M., Jiang Y., Zeng K.W, & Tu P.F. (2017). Highly Selective Activation of Heat Shock Protein 70 by Allosteric Regulation Provides an Insight into Efficient Neuroinflammation Inhibition. EBioMedicine, 23, 160-172.

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Investigating BAMBI and CTGF Knockdown in Multiple Myeloma

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ON-TARGETplus Human CTGF siRNA-SMART pool, ON-TARGETplus Human BAMBI siRNA-SMART pool, and ON-TARGETplus non-targeting Pool siRNA were purchased from Dharmacon (Lafayette, CO, USA) and were used for all transfection experiments. BAMBI siRNA(h) #sc-60243, a pool of 3 target-specific siRNAs (Santa Cruz Biotechnology, Dallas, TX, USA) was only used to confirm the effect of knockdown BAMBI expression on MM cell proliferation. The oligonucleotide sequences of the siRNAs in each pool are shown in (Table S1).
An antibody against BAMBI/NMA (ab203070) was purchased from Abcam (Cambridge, UK), anti-CTGF (sc-14939) and anti-goat IgG (sc-2354) from Santa Cruz Biotechnology (Dallas, TX, USA), and antibodies against cyclin D1 (92G2), cyclin D3 (2936), CDK2 (2546), CDK4 (1279), phospho-Smad2 (Ser465/467), phospho-Smad3 (Ser423/425) (C25A9), Smad2/3, rabbit IgG (7074), and mouse IgG (7076) from Cell Signaling Technology (Danvers, MA, USA). Anti-β-actin (A5441) was obtained from Sigma-Aldrich (St. Louis, MO, USA).

Hoang N.T., Hassan G., Suehiro T., Mine Y., Matsuki T, & Fujii M. (2022). BMP and activin membrane-bound inhibitor regulate connective tissue growth factor controlling mesothelioma cell proliferation. BMC Cancer, 22, 984.

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Protein Expression Analysis Protocol

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Tissues or tumor cells were lysed with 2% sodium dodecyl sulfate (SDS) and the protein concentration was then measured with BCA protein assay kit. Next, proteins were separated by SDS-PAGE gel and transferred to a nitrocellulose membrane. After blocking, the membrane was incubated with different primary antibodies and secondary antibody (with washing between steps). Finally, the target protein bands were visualized via chemiluminescence system. Primary antibodies include: CDCA5 (ab192237) and eEF1A1 (ab157455) obtained from Abcam; p-AKT (Ser473) antibody (9271), p-S6 (Ser240/244) antibody (5364) and p-4EBP1 (Thr37/46) antibody (2855) were purchased from Cell Signaling Technology; β-actin (sc-69879) and GAPDH (sc-32233) were obtained from Santa Cruz. Secondary antibody Rabbit IgG (#7074) and Mouse IgG (#7076) were bought from Cell Signaling Technology.

Wang X., Shi A., Liu J., Kong W., Huang Y., Xue W., Yang F, & Huang J. (2024). CDCA5-EEF1A1 interaction promotes progression of clear cell renal cell carcinoma by regulating mTOR signaling. Cancer Cell International, 24, 147.

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Carnosic Acid Modulates NF-κB Signaling

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Carnosic acid (C₂₀H₂₈O₄; molecular weight 332.4400) was obtained from Baoji Herbest Bio-Tech Co., Ltd. (Shanxi, China) and the purity (above 98%) was affirmed by HPLC and MS data. Lipopolysaccharide (LPS) from Escherichia coli O55:B5 was from Sigma-Aldrich (St. Louis, MO, United States). MG132 was from Beyotime Biotechnology (Jiangsu, China). Antibodies against COX2 (12282), GAPDH (3683), p-IKKα/β (2697), IKKα (11930), IKKβ (8943), p-IκB-α (2859), IκB-α (4814), p-NF-κB p65 (3033), NF-κB p65 (8242), p-ERK1/2 MAPK (4370), p-SAPK/JNK (4668), SAPK/JNK (9252), p-p38 MAPK (4511), p38 MAPK (8690), Histone H3 (4499), α-Tubulin (9099), rabbit IgG (7074), and mouse IgG (7076) were purchased from Cell Signaling Technology (Beverly, MA, United States). Antibody against ERK1/2 MAPK (16443-1-AP) and ERK4 (26102-1-AP) was bought from Proteintech Group (Chicago, IL, United States). Antibodies against FKHR/FoxO1 (BS5518), FoxO3 (BS3574), ERK3 (BS2662), and p-ERK3/4 (BS6377) were from Bioworld Technology (St. Louis Park, MN, United States).

Wang L.C., Wei W.H., Zhang X.W., Liu D., Zeng K.W, & Tu P.F. (2018). An Integrated Proteomics and Bioinformatics Approach Reveals the Anti-inflammatory Mechanism of Carnosic Acid. Frontiers in Pharmacology, 9, 370.

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