Animals were incubated in 30 ml of sea water containing 1μM Sphingosine-Kinase-Inhibitor (A) SKI-I (Abcam), 14μM Sphingosine-Kinase-Inhibitor (B) CAS 1177741-83-1 (Millipore 567731), or 10 μM S1PR1 antagonist W146 (Tocris). Seawater containing drugs was replaced and fresh food (algae) was added daily. Controls were incubated in sea water without inhibitors with vehicle ethanol 0.1% or DMSO 0.001%. After 4 days, animals were fixed and analyzed by in situ hybridization as described above. For each treatment, two genetically identical colonies were treated simultaneously, and the experiment was performed three times. Buds containing vasa-positive cells and the number of vasa-positive cells in the vasculature were counted on each treated and untreated colony under an epifluoresence microscope. Data are expressed as averages from three experiments, and statistical analysis was performed using a paired, two-sided Student’s t-test.
Cas 1177741 83 1
Manufactured by Merck Group
CAS 1177741-83-1 is a chemical compound used in laboratory research and development. It is a powder form product with a molecular formula and weight. This information is provided without further interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cas 1177741 83 1
1
Investigating Sphingosine Kinase Inhibitors in Cnidarian Stem Cell Regulation
2
Influence of Sphingolipid Signaling on Vasa Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animals were incubated in 30 ml of sea water containing 1 μM Sphingosine Kinase Inhibitor (A) SKI-I (Abcam), 14 μM Sphingosine Kinase Inhibitor (B) CAS 1177741-83-1 (Millipore 567731) or 10 μM S1PR1 antagonist W146 (Tocris). Sea water containing drugs was replaced and fresh food (algae) was added daily. Controls were incubated in sea water without inhibitors with vehicle 0.1% ethanol or 0.001% dimethylsulphoxide. After 4 days, animals were fixed and analysed by in situ hybridization as described above. For each treatment, two genetically identical colonies were treated simultaneously, and the experiment was performed three times. Buds containing vasa-positive cells and the number of vasa-positive cells in the vasculature were counted on each treated and untreated colony under an epifluoresence microscope. Data are expressed as averages from three experiments, and statistical analysis was performed using a paired, two-sided Student's t-test.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!