The glucose and acetic acid in the hydrolyzate were determined by a method of HPLC. The liquid phase fraction was filtered through a 0.22 μm filter prior to analysis, and then 20 μL of sample was injected. A HPLC method was carried out using a Bio-Rad (USA) Aminex® HPX-87H column (300 mm × 7.8 mm, Organic Acid Analysis Column) at 65 °C and 0.005 M sulfuric acid as a mobile phase (flow rate was 0.6 mL min−1). A refraction index detector (Shimadzu, Japan) was used for sugars and acetic acid analysis. The contents of furfural, HMF, ferulic acid, and p-coumaric acid were determined by a HPLC method using a diode array detector (Shimadzu, Japan) and an InertSustain C18 column at 40 °C. Acetic acid (0.5 wt%) and methanol ratio of 9 : 1 (v/v) at flow rate of 1 mL min−1 was used as mobile phase, the detection wavelength was 280 nm and the injection volume was 20 μL.
Diode array detector
Manufactured by Shimadzu
Sourced in Japan
The diode array detector is a component used in analytical instrumentation, such as high-performance liquid chromatography (HPLC) and other separation techniques. Its core function is to measure the absorption of light by the sample components as they elute from the separation column. This data can then be used to identify and quantify the individual components in the sample.
Automatically generated - may contain errors
Lab products found in correlation
C18 column, by Merck Group (2 mentions)
Hplc system, by Shimadzu (2 mentions)
Ods a column, by YMC (1 mentions)
Lc 20a hplc system, by Shimadzu (1 mentions)
Inertsil ods 3 c18 column, by GL Sciences (1 mentions)
Shim pack xr ods column, by Shimadzu (1 mentions)
Lc 20a system, by Shimadzu (1 mentions)
β carotene standard, by Fujifilm (1 mentions)
Flexigene dna kit, by Qiagen (1 mentions)
7500 real time pcr system, by Thermo Fisher Scientific (1 mentions)
Geneamp pcr system 9700, by Thermo Fisher Scientific (1 mentions)
Custom taqman snp genotyping assay, by Thermo Fisher Scientific (1 mentions)
Unity inova, by Agilent Technologies (1 mentions)
Ngc chromatography system, by Bio-Rad (1 mentions)
Cdpcho, by Merck Group (1 mentions)
P cho, by Merck Group (1 mentions)
Lc 20at system, by Shimadzu (1 mentions)
Ptfe membrane, by Merck Group (1 mentions)
Kinetex c18 column, by Phenomenex (1 mentions)
Uflc lc 20ad, by Shimadzu (1 mentions)
18 protocols using diode array detector
1
Hydrolyzate Composition Analysis by HPLC
2
Soil Metabolite Extraction and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the treatment with 10 μmol BOA, gramine or quercetin, the metabolites were extracted from 300 g of soil with methanol containing 0.1% formic acid. The soil/methanol mixture was sonicated for 5 min followed by an incubation under vigorous shaking for 30 min. The slurry was centrifuged at 4,000 × g for 10 min. The organic phase was harvested and concentrated using a rotary evaporator. The metabolites were analyzed by HPLC with a diode array detector (Shimadzu) with an analytical reversed phase C18 column (Nucleodur, Macherey-Nagel, Germany) as described by Schulz et al. (2018) (link).
3
Quantification of Brassicaceae Bioactives
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ITCs are the main bioactive component in the plant family Brassicaceae (Cruciferae) and as reported in Thai rat-tailed radish12 –14 ,59 (link). The level of ITCs (i.e., sulforaphene and sulforaphane) were determined in both conventional extracts, and EVs derived from the microgreens using HPLC. The HPLC analysis of sulforaphene and sulforaphane was performed as reported11 ,13 , using a Prominence-i HPLC (LC–2030C 3D, Shimadzu, Kyoto, Japan) equipped with a diode array detector (Shimadzu, Kyoto, Japan). The stationary phase and guard column comprised the HiQ sil C18W column (4.6 mm × 250 mm, 5 µm) (KYA Technologies Corporation, Tokyo, Japan). The column temperature was set at 25 °C with a flow rate of 1 mL/min. The mobile phase was isocratic elution with 5% tetrahydrofuran (THF) and 95% ultrapure water. The detection wavelength was set at 210, 245, and 254 nm. Labsolution software (version 5.73, Kyoto, Japan) was used to obtain the chromatograms. The DCM dry extract was dissolved in DMSO. The injection volume was 20 µL. Sulforaphene and sulforaphane standard compounds were used to identify the presence of these compounds in the samples by comparing the retention time of the standard peak with the samples. The sulforaphene and sulforaphane contents in the DCM extract and EVs were calculated from the peak height compared to the standard compounds.
4
Quantification and Identification of Flavonoids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The quantification and identification of flavonoids in extracts were carried out in a Shimadzu HPLC system by the method of Martinez-Cruz and Paredes-López [22 (link)]. A diode array detector (Shimadzu) was used. Phenolic compounds were separated using a Supelco C18 column, 5 μm, 150 mm × 4.6 mm. The separation was achieved as follows: the mobile phase consisted of 2% acetic acid in water (solvent A) and 2% acetic acid, 30% acetonitrile, and 68% water (solvent B). All solvents were filtered through a 0.45 μm membrane prior to analysis. The system was run with the following gradient program: 0–6 min, 0–10% B; 6–10 min, 10–15% B; 10–16 min, 15–40% B; 16–30 min, 40–100% B; and 3 min, 100% B. The flow rate was kept constant at 1.0 mL/min. The injection volume for extracts and standards was 10 μL. The standards were dissolved with 70% acetone in distilled water to give serial concentrations in a range of 0.0001–0.1 mg/mL for flavonoids standards. The standard curves were prepared using the peak areas of different concentrations (mg/mL, x-axis), and were expressed by the linear least-squares regression equation. All measurements were performed in triplicate for each assay, and the results were expressed as the mean ± standard deviation.
5
Isolation and Characterization of SS-A Degradation Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Purified degradation compounds of SS-A, for structural evaluation using spectra analyses were isolated from the extract by means of repeated semi-preparative HPLC (Chuangxin Tongheng Co. Ltd., Beijing, China). The column used was a YMC-Pack ODS-A Column (5 μm, 20 mm × 250 mm, i.d.). The mobile phase was (Tmin/A:B; T0/30:70; T20/40:60; T40/35:65; T50/30:70) with detection wavelengths of 210 and 254 nm. The flow rate was set at 5.0 mL·min−1, and the injection volume was 500 μL. The purity of the compounds was examined by analytical HPLC–DAD (DiodeArray Detector, Shimadzu).
6
HPLC Analysis of Dihydroflavonols and Anthocyanidins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HPLC analysis was conducted on an LC-20A HPLC system with a diode array detector (Shimadzu, Kyoto, Japan). The separation of dihydroflavonol substrates and anthocyanidin products was accomplished on an Inertsil-ODS3 C18 column (5 μm, 250 × 4.6 mm, GL Science). The mobile phase consisted of 0.1% (v/v) formic acid (A) and acetonitrile containing 0.1% (v/v) formic acid (B). The gradient profile was optimized as follows: 0 min, 95% A/5% B; 30 min, 45% A/55% B; 45 min, 35% A/65% B; 50 min, 0% A/100% B; 52 min, 95% A/5% B; and 60 min, 95% A/5% B. The flow rate was 1 mL·min−1, and the column temperature was maintained at 30 °C. The detection wavelength was 288 nm for dihydroflavonols and 520 nm for anthocyanidins.
7
HPLC Quantification of Phenolic Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The quantification and identification of phenolic acids in extracts were carried out in a Shimadzu HPLC system by the method of Martinez-Cruz and Paredes-López [22 (link)]. A diode array detector (Shimadzu) was used. Phenolic compounds were separated using a Supelco C18 column, 5 μm, 150 mm × 4.6 mm. The separation was achieved as follows: the mobile phase consisted of water (solvent A) and 70% aqueous acetonitrile (solvent B). All solvents were filtered through a 0.45 μm membrane prior to analysis. The system was run with the following gradient program: 0–4 min, 0–10% B; 4–8 min, 10–15% B; 8–17 min, 15–40% B; 17–35 min, 40–100% B; and 3 min, 100% B. The flow rate was kept constant at 1.0 mL/min. The injection volume for extracts and standards was 10 μL. The standards were dissolved with 70% acetone to give serial concentrations in a range of 0.0004150–0.1950 mg/mL for phenolic acids. The standard curves were prepared using the peak areas of different concentrations (mg/mL, x-axis) and were expressed by the linear least-squares regression equation. All measurements were performed in triplicate for each assay, and the results were expressed as the mean ± standard deviation.
8
HPLC Analysis of Triterpenoid Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the chemicals were analyzed by High Performance Liquid Chromatography (HPLC). A Shimadzu LC20A system equipped with a LC20ADXR pumper, an auto-sampler and a diode array detector (Shimadzu, Kyoto, Japan) was used for HPLC analysis. Chromatographic separation of CK, DMG, DM and PPD was carried out on a Shim-pack XR-ODS column (100 mm × 2.0 mm, 2.2 μm, Shimadzu, Kyoto, Japan) at 35 °C. The mobile phase consisted of water (A) and acetonitrile (B), and separation was conducted using the following gradient procedure: 0–2.5 min (60% B), 2.5–10 min (60%–90% B), 10–11 min (90% B), 11–13 min (60% B) and the flow rate was maintained at 0.45 mL/min. The triterpenoid products were detected at 203 nm. Calibration curves of standard samples based on HPLC integrated peak were generated and used for the quantification of CK, DMG, DM and PPD.
9
Quantitative HPLC Analysis of Capsanthin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Qualitative and quantitative HPLC analysis was performed according to the modified method of Goda et al. (1995) using a Shimadzu LC-10A quaternary pump equipped with a diode array detector (Shimadzu, Kyoto, Japan) and a Cosmosil 5C18-AR II reverse-phase column (Nacalai Tesque, Kyoto, Japan) protected by a guard cartridge (Nacalai Tesque). The oven was operated at 40°C. The sample injection volume was 10 μL. Samples were eluted with acetone in water as follows: 70% acetone for 5 min; 70%–90% linear gradient for 5 min; 90% acetone for 3 min; 90%–100% linear gradient for 20 min; and 100% acetone for 5 min. The flow rate was 1.0 mL/min. For quantification, a capsanthin standard was obtained from Extrasynthese S.A. (Lyon, France) and a β-carotene standard was obtained from Wako Pure Chemical Industries (Osaka, Japan). All samples were analyzed before and after saponification. Loss of capsanthin during saponification was calculated from the loss of β-carotene. All analysis was carried out in 3 to 5 replications.
10
Pharmacogenomic Analysis of Antiepileptic Drugs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma lamotrigine was measured using a validated high-performance liquid chromatography with a diodearray detector (Shimadzu, Japan), as described previously [30] , while serum valproate was measured by an immunoassay (PETINIA) on a Dimension Expand analyzer (Siemens; calibrator and control samples by Siemens, Germany). Both analytes are included in external quality control schemes (DGKL RfB and UK NEQAS).
Genomic DNA was extracted from three milliliters of whole blood using the FlexiGene DNA Kit (Qiagen, Hilden, Germany), according to the manufacturer's instructions. Genotyping of MDR1/ABCB1 1236C>T, ABCG2 421C>A and UGT2B7 -161C>T was performed using TaqMan Drug Metabolism Genotyping assays ID C_7586662_10, ID C_15854163_70, ID C_27827970_40, respectively, while genotyping of UGT1A4*3 c.142 T>G was performed using Custom TaqMan SNP Genotyping assay (Applied Biosystems, Foster City, CA, USA) by real-time polymerase chain reaction (PCR) genotyping method on the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA), according to the manufacturer's instructions. Genotyping of UGT1A4*3 c.142T>G was con rmed by a PCR-RFLP method on the Gene Amp PCR System 9700 (Applied Biosystems, Foster City, CA, USA) [31] .
Genomic DNA was extracted from three milliliters of whole blood using the FlexiGene DNA Kit (Qiagen, Hilden, Germany), according to the manufacturer's instructions. Genotyping of MDR1/ABCB1 1236C>T, ABCG2 421C>A and UGT2B7 -161C>T was performed using TaqMan Drug Metabolism Genotyping assays ID C_7586662_10, ID C_15854163_70, ID C_27827970_40, respectively, while genotyping of UGT1A4*3 c.142 T>G was performed using Custom TaqMan SNP Genotyping assay (Applied Biosystems, Foster City, CA, USA) by real-time polymerase chain reaction (PCR) genotyping method on the 7500 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA), according to the manufacturer's instructions. Genotyping of UGT1A4*3 c.142T>G was con rmed by a PCR-RFLP method on the Gene Amp PCR System 9700 (Applied Biosystems, Foster City, CA, USA) [31] .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!