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Formic acid hplc grade

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Formic acid (HPLC grade) is a colorless, volatile liquid chemical compound with the formula HCOOH. It is primarily used as a mobile phase component in high-performance liquid chromatography (HPLC) applications to facilitate the separation and analysis of various analytes.

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Lab products found in correlation

Hplc grade acetonitrile, by Merck Group (10 mentions) Hplc grade methanol, by Merck Group (6 mentions) Acetonitrile, by Merck Group (5 mentions) Methanol, by Thermo Fisher Scientific (5 mentions) Milli q water purification system, by Merck Group (4 mentions) Mili q water purification system, by Merck Group (3 mentions) Acetonitrile, by Tedia (3 mentions) Milli q system, by Merck Group (3 mentions) Milli q reagent water system, by Merck Group (3 mentions) Hplc grade acetonitrile, by Thermo Fisher Scientific (3 mentions) Gallic acid, by Chengdu Must Bio-Technology (2 mentions) Emodin, by Chengdu Must Bio-Technology (2 mentions) Physcion, by Chengdu Must Bio-Technology (2 mentions) Proanthocyanidin b1, by Chengdu Must Bio-Technology (2 mentions) Proanthocyanidin b2, by Chengdu Must Bio-Technology (2 mentions) Methanol, by Merck Group (2 mentions) Milli q purification system, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Tedia (2 mentions) Ammonium acetate, by Tedia (2 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)

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Formic acid (117 citations) HPLC grade (19 citations)

33 protocols using formic acid hplc grade

1

Authentication and Chemical Standards for Polygonum multiflorum

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The PMR materials were collected from three different habitats in China, and three replicate sample materials were from different biological plants (Table 4). All samples were authenticated as the tuberous roots of Polygonum multiflorum Thunb. by Prof. Zhong-Zhen Zhao from the School of Chinese Medicine, Hong Kong Baptist University. Voucher specimens were deposited in the Bank of China (Hong Kong) Chinese Medicines Centre of Hong Kong Baptist University.
Chemical standards for trans-THSG, emodin, physcion, catechin, proanthocyanidin B1, proanthocyanidin B2, and gallic acid were purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China); standards for cis-THSG, epicatechin, epicatechin-3-gallate, emodin-8-O-β-D-glucoside, and physcion-8-O-β-D-glucoside were purchased from Chengdu Xunchen Biological Technology Co., Ltd. (Chengdu, China). HPLC-grade methanol and acetonitrile (E. Merck, Darmstadt, Germany), ammonium acetate (Sigma-Aldrich, St. Louis, MO, USA), HPLC grade formic acid (Tedia, Fairfield, OH, USA) were purchased. Ultra-pure water was prepared by a Mili-Q water purification system (Millipore, Burlington, MA, USA).
Liang L., Xu J., Liang Z.T., Dong X.P., Chen H.B, & Zhao Z.Z. (2018). Tissue-Specific Analysis of Secondary Metabolites Creates a Reliable Morphological Criterion for Quality Grading of Polygoni Multiflori Radix. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(5), 1115.
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2

HPLC Analysis of Mesaconitine and Diazepam

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10-hydroxy mesaconitine (purity >98%) and diazepam (purity >98%, internal standard) were purchased from Chengdu Mansite Biotechnology Co., Ltd. (Chengdu, China). HPLC-grade formic acid, methanol, and acetonitrile were purchased from Tedia Company, Inc. (Ohio, USA). Ultrapure water was prepared using a Millipore Milli-Q water purification system (Bedford, MA, USA).
Yang J., Zeng G., Ma J., Wang X, & Zhou Q. (2021). Pharmacokinetics of 10-Hydroxy Mesaconitine in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Quadrupole Mass Spectrometry. Journal of Analytical Methods in Chemistry, 2021, 6640184.
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3

Quantitative HPLC Analysis of FLC and KCZ

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FLC and ketoconazole (KCZ) (> 99.0%) were purchased from Sigma-Aldrich (St Louis, MO, USA). Acetonitrile was liquid chromatography (LC) grade and purchased from Merck (Darmstadt, Germany). HPLC-grade formic acid was purchased from Tedia Company (Fairfield, OH, USA). Dichloromethane, sodium hydroxide and dimethyl sulfoxide were purchased from Shanghai Chemical Reagent Company (Shanghai, China). Deionized water was prepared from Milli-Q water purifying system (Millipore Corporation, Bedford, MA, USA). Methanol was purchased from Merck (Darmstadt, Germany).
Wang X., Wang X., Cai T., Qin Y., Li L., Jiang Y., Han B, & Cao Y. (2022). Development and validation of a sensitive LC-MS/MS method for determination of intracellular concentration of fluconazole in Candida albicans. Frontiers in Microbiology, 13, 1007576.
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4

Neurochemical Markers Analysis Protocol

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3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and corticosterone were purchased from Sigma (St. Louis, MO, USA). Dulbecco’s Modified Eagle culture medium (DMEM), fetal bovine serum (FBS), heat-inactivated horse serum, penicillin, and streptomycin were purchased from Gibco (Grand Island, NY, USA). Lactate dehydrogenase (LDH) assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Antibodies against Phospholipase A2 IIA (PLA2), calcium/calmodulin-dependent protein kinase II (CaMK II), pro-caspase-3, BDNF, and GAPDH were supplied by Epitomics (Epitomics Inc., Burlingame, USA). High Performance Liquid Chromatography (HPLC) grade dimethyl sulfoxide (DMSO), acetonitrile, and methanol were purchased from Fisher (New Jersey, USA). HPLC-grade formic acid was purchased from Tedia (Fairield, USA). Ultrapure water (18.2 MΩ) was prepared with a Milli-Q water purification system (Millipore, France). All other chemicals and reagents were of analytical grade.
Jia H., Liu Y., Yu M., Shang H., Zhang H., Ma L., Zhang T, & Zou Z. (2019). Neuroprotective Effect of Cyperi rhizome against Corticosterone-Induced PC12 Cell Injury via Suppression of Ca2+ Overloading. Metabolites, 9(11), 244.
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5

TBMS-I and Ardisiacrispin A Protocol

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TBMS-I (purity >98%; Figure 1(a)) and ardisiacrispin A (internal standard, purity >98%; Figure 1(b)) were purchased from Chengdu Mansite Biotechnology Co., Ltd. (Chengdu, China). HPLC-grade methanol and acetonitrile were bought from Merck (Darmstadt, Germany). HPLC-grade formic acid was supplied by Tedia (Ohio, USA). A Milli-Q system (Millipore, Bedford, USA) is used for generating ultrapure water. The ICR mice (male, weight 20–22 g, n=12) obtained were from the Laboratory Animal Center of Wenzhou Medical University (Wenzhou, China).
Chen L., Weng Q., Li F., Liu J., Zhang X, & Zhou Y. (2018). Pharmacokinetics and Bioavailability Study of Tubeimoside I in ICR Mice by UPLC-MS/MS. Journal of Analytical Methods in Chemistry, 2018, 9074893.
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6

Quantitative Analysis of Monocrotaline and Rhynchophylline

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Monocrotaline (purity > 98%, Figure 1(a)) and rhynchophylline (IS, purity > 98%, Figure 1(b)) were purchased from Chengdu Mansite Pharmaceutical Co. Ltd. (Chengdu, China). HPLC-grade formic acid and organic solvents (acetonitrile and methanol) were purchased from Tedia (Ohio, USA) and Merck (Darmstadt, Germany), respectively. Purified water was obtained with a Milli-Q purification system (Millipore, Bedford, MA, USA).
Chen L., Zhang B., Liu J., Fan Z., Weng Z., Geng P., Wang X, & Lin G. (2018). Pharmacokinetics and Bioavailability Study of Monocrotaline in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry. BioMed Research International, 2018, 1578643.
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7

Characterization of P. notoginseng

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Raw material of P. notoginseng was collected from Yunnan Province of China. No specific permissions were required for the described field studies. The locations are neither privately owned nor protected by the Chinese government. No endangered or protected species were sampled. Reference substances of notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, and ginsenoside Rd were purchased from Shanghai Winherb Medical Technology Co., Ltd (Shanghai, China). Analytical grade ethanol was purchased from Shanghai Lingfeng Chemical Reagents Co., Ltd (Shanghai, China). Analytical grade methanol was purchased from Sinophram Chemical Reagent Co., Ltd. HPLC-grade acetonitrile was purchased from Merck (Darmstadt, Germany). HPLC-grade formic acid was purchased from Tedia Company (Fairfield, USA). Deionized water was produced from Milli-Q academic water purification system (Milford, MA, USA). HPD-100 resin was purchased from Cangzhou Bon Adsorber Technology Co., Ltd. Thrombin was purchase from Beijing Solarbio Science & Technology Co., Ltd. Thrombin generation chromogenic substrate (T3068) was purchase from Sigma-Aldrich (Buchs, Switzerland). β-Ala-Gly-Arg-p-nitroaniline was purchased from Merck (Darmstadt, Germany).
Pan J.J., He S.Y., Shao J.Y., Li N., Gong Y.Q, & Gong X.C. (2019). Critical pharmaceutical process identification considering chemical composition, biological activity, and batch-to-batch consistency: A case study of notoginseng total saponins. Chinese Herbal Medicines, 12(1), 29-35.
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8

Simultaneous Quantification of Anticancer Agents

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All analytes including Cap (Lot: J0713AS), 5'-DFCR (Lot: J1112AS), 5'-DFUR (Lot: J0713A), 2'-DFUR (Lot: J0620A), 5-FU (Lot: A0930AS), fludarabine (Fdb) (Lot: M0501AS) and 5-chlorouracil (5-ClU) (Lot: J1204A) but excepting FUH2 (Lot: 5-PTR-167-1) were purchased from Meilun Biotech Co., Ltd (Dalian City, China). FUH2 was supplied by Toronto Research Chemicals (Toronto, Canada). HPLC-grade acetonitrile was obtained from Merck (Merck Company, Darmstadt, Germany). HPLC-grade formic acid, dimethyl sulfoxide (DMSO), and ammonium acetate were purchased from Tedia Company Inc (Tedia, Fairfield, OH, USA). Ultrapurified water (0.22 µm filter membrane) was self-made in laboratory on a Milli-Q Reagent Water System (Millipore, MA) and was used throughout. HPLC-grade isopropanol and ethyl acetate were bought from Thermo fisher (Thermo Fisher Scientific, Waltham, MA) and Caledon laboratories (Caledon Laboratories Ltd, Georgetown, Canada). Human blank plasma was donated by Shanghai Red Cross Blood Center (Shanghai, China).
Wang Z., Li X., Yang Y., Zhang F., Li M., Chen W., Gao S, & Chen W. (2019). A Sensitive and Efficient Method for Determination of Capecitabine and Its Five Metabolites in Human Plasma Based on One-Step Liquid-Liquid Extraction. Journal of Analytical Methods in Chemistry, 2019, 9371790.
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9

Ginseng Extract Modulates RAW264.7 Cells

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Ginseng root extract was purchased from ZeLang Healthtech Co. Ltd., China. Reference ginsenosides, including ginsenoside Rb1, Re, Rg1, Rd, Gyp XVII, Rg2, F1, Rh1 and PPT were purchased from Jilin University (Changchun, China). Acetonitrile and methanol of HPLC grade were obtained from Fisher Scientific (Waltham, MA). HPLC grade formic acid (96%) was obtained from Tedia (Fairfield, OH). Other reagents were of analytical purity.
The RAW264.7 cell line was purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). Dulbecco’s modified Eagle’s medium (DMEM) was obtained from Gibco (Carlsbad, USA). Lipopolysaccharides (LPS) was purchased from Sigma (Missouri, USA). A Cell Counting Kit-8 (CCK-8) and enzyme-linked immunosorbent assay (ELISA) kit were obtained from Bestbio (Shanghai, China) and R&D (Minneapolis, USA).
Yu S., Zhou X., Li F., Xu C., Zheng F., Li J., Zhao H., Dai Y., Liu S, & Feng Y. (2017). Microbial transformation of ginsenoside Rb1, Re and Rg1 and its contribution to the improved anti-inflammatory activity of ginseng. Scientific Reports, 7, 138.
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10

Isolation and Characterization of Pyrrolizidine Alkaloids from Senecio and Ligularia Species

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The standard references including Adonifoline, Isoline, Clivorine, and Senkirkine were isolated by our lab from Senecio scandens, Ligularia duciformis, Ligularia hodgsonii, and Senecio solidagineus, respectively [6 (link)–9 (link)]. Monocrotaline, isolated from Crotalaria assamica, was kindly supplied by Professor Zhiben Tu (Wuhan Botanical Institute, Academia Sinica, Wuhan, China). Their structures were confirmed using UV, IR, NMR, and MS analysis, and HPLC analysis showed that the purity of all standard references was 98%. Among five Senecio plants, the herbs of Senecio nemorensis, Senecio cannabifolius, and Senecio cannabifolius var. integrifolius were collected from Jilin province. The herbs of Senecio vulgaris and Senecio scandens were collected from Heilongjiang province and Guangdong province, respectively. Moreover, the harvesting place of the herb Ligularia duciformis was Gansu province. All samples were identified by Dr. Lihong Wu and voucher specimens were deposited at the Herbarium of Shanghai R&D Center for Standardization of TCM. HPLC-grade acetonitrile was purchased from Sigma-Aldrich (St. Louis, MO, USA). HPLC-grade formic acid was purchased from Tedia Company (Fairfield, OH, USA). HPLC-grade water was produced with a Milli-Q water purifying system. All other reagents were purchased from commercial source.
Lu A.J., Lu Y.L., Tan D.P., Qin L., Ling H., Wang C.H, & He Y.Q. (2021). Identification of Pyrrolizidine Alkaloids in Senecio Plants by Liquid Chromatography-Mass Spectrometry. Journal of Analytical Methods in Chemistry, 2021, 1957863.
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