Fmoc met oh

PL-Wang resin (0.4 mmole/g) was obtained from Varian, Inc. (Shropshire, UK). Fmoc-Ser(tBu)-OH, Fmoc-Met-OH, Fmoc-Leu-OH, Fmoc-Ile-OH, Fmoc-Ala-OH, Fmoc-Lys(Boc)-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Asn(trt)-OH, Fmoc-Glu(OtBu)-OH, Fmoc-Phe-OH, Fmoc-Gln(trt)-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Arg(pbf)-OH, N,N′-diisopropylcarbodiimide (DIC), 4-dimethylaminopyridine (DMAP) and triisopropylsilane (TIS) were obtained from GL Biochem Ltd. (Shanghai, China). Fmoc-Val-OH, Fmoc-Gly-OH, Fmoc-His(trt)-OH and N,N,N′,N′-tetramethyl-uronium-hexafluoro-phosphate (HBTU) were obtained from CS Bio Co. (Menlo Park, USA). Dimethylformamide (DMF), dichloromethane (DCM) and dimethyl sulfoxide (DMSO) were obtained from Daejung Chemicals & Metals Co., Ltd. (Siheung, Korea). Anisole, piperidine and N,N-diisopropylethylamine (DIEA) were obtained from Sigma-Aldrich (St. Louis, USA). Trifluoroacetic acid (TFA) was obtained from Yakuri Pure Chemicals Co., Ltd. (Kyoto, Japan). Ether, Anhydrous was obtained from J.T. Baker (Center Valley, USA). Zoletil® (Virbac) and Rompun® (Bayer Parma) were obtained from SMP animal medicine (Suwon, Korea). Protease inhibitor cocktail was obtained from Roche Diagnostics (IN, USA). EDTA treated BD vacutainer® was obtained from Becton, Dickinson and Company (NJ, USA). Human Aβ42 Ultrasensitive ELISA kit was obtained from Invitrogen (CA, USA).

Hydroxybenzotriazole, 2-(7-Aza-1H-benzotriazole-1-yl)-1, 1, 3, 3-tetramethyluronium hexafluorophosphate, and diisopropylethylamine were purchased from Sigma (St. Louis, MO, USA). PdCl₂(PPh₃)₂ and Bu₃SnH were provided by J&K Scientific (Beijing, China); the MTT one-step assay kit based on MTT reduction assay was provided by Hope Biotechnology (Tianjin, China). HepG2, HCT116, MCF7, and H1299 cell lines were obtained from the cell bank of the Chinese Academy of Sciences (Shanghai, China) and identified by the National Cell Resource Center (Shanghai, China). Parent and MDR1 transfected MDCK cells were obtained from the State Key Laboratories of Drug Delivery Technology and Pharmacokinetics (Tianjin, China). Doxorubicin was purchased from Lark Technology (China, Beijing). Fmoc-Asp-O-allyl, Fmoc-Leu-OH, Fmoc-Met-OH, Fmoc-Phe-OH, Fmoc-Tyr-OH, Fmoc-Val-OH, and Fmoc-Ile-OH were all obtained from GL Biochem (Shanghai, China). All chemicals used for tissue culture were purchased from Gibco BRL (Grand Island, NY, USA).