Lungs were minced and incubated in DME + DNAse + collagenase A (Roche) rotating at 37°C for two hours. Dissociated lungs were then washed twice with PBS, and filtered through a 70 µm and 40 µm cell strainer. Cells were stained with CD45 FITC (eBioscience), CD11b PerCP-Cyanine 5.5 (eBioscience); F480 PE-Cyanine7 (Affymetrix); CCR2 APC-Cy7 (BioLegend); Ly6C e450 (Affymetrix); CD45 PE-Cyanine7 (Affymetrix); CD11b PerCP-Cyanine5.5 (eBioscience); Ly6C e450 (Affymetrix); Ly6G FITC (BioLegend); CD29 (Affymetrix) and CD24 (Affymetrix). After incubating on ice for 45 minutes, cells were washed twice in PBS with 0.1% BSA, acquired on a Fortessa (BD Biosciences) and analyzed using FACSDiva (BD Biosciences).
Ly6c e450
Manufactured by Thermo Fisher Scientific
The Ly6C e450 is a lab instrument designed for the detection and analysis of the Ly6C antigen. It utilizes flow cytometry technology to provide precise measurements of Ly6C expression on cell samples. The Ly6C e450 is a core tool for researchers studying hematopoietic and immune cell populations.
Automatically generated - may contain errors
1 protocol using ly6c e450
1
Lung Immune Cell Isolation
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