Cross-linked and sorted chromatin from adult ex vivo isolated MSN nuclei (6 × 106 nuclei for each ChIP) was immunoprecipitated with antibody against H3K27me3 followed by immunoprecipitation with antibody against H3K4me3 using the Re-ChIP-IT sequential ChIP kit from Active Motif (cat# 53016) following manufacturers recommendations. The eluted chromatin was extracted and purified as described above (see “Chromatin Immunoprecipitation”). In addition, a control sequential ChIP was carried out in which chromatin was immunoprecipitated first with antibody against H3K27me3 followed by antibody against IgG.
Re chip it sequential chip kit
Manufactured by Active Motif
The Re-ChIP-IT sequential ChIP kit is a tool for performing sequential chromatin immunoprecipitation (re-ChIP) experiments. It enables the detection of protein complexes or co-localized proteins on chromatin.
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2 protocols using re chip it sequential chip kit
1
Sequential ChIP-seq of H3K27me3 and H3K4me3
2
Sequential ChIP-seq of H3K27me3 and H3K4me3
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