Salmon calcitonin sct

Salmon calcitonin (sCT) was purchased from Bachem AG (Bubendorf, Switzerland). The peptide CRQIKIWFQNRRMKWKK (Cys-penetratin) was synthesized and purchased from Peptron Co., Ltd. (Daejeon, Republic of Korea). The lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[4-(p-maleimidophenyl) butyramide] (MPB-PE) was purchased from Avanti Polar Lipids (Alabaster, AL). L-α-lecithin (97.7% phosphatidylcholine) was purchased from Merck Millipore (Billerica, MA, USA). Tween 80^® was purchased from Duksan (Ansan, Republic of Korea). An sCT ELISA kit was purchased from Phoenix Pharmaceuticals (Burlingame, CA, USA). A Ham-F-12 Nutrient mixture (Ham’s F-12) was purchased from Welgene (Gyeongsan-si, Republic of Korea). A CellTiter 96^® Aqueous One Solution Cell Proliferation Assay kit (MTS) was purchased from Promega (Madison, WI, USA). Alexa Fluor 647 NHS Ester, SnakeSkin^TM dialysis tubing (3.5 K MWCO), fetal bovine serum (FBS), 0.25% trypsin-EDTA, and penicillin/streptomycin were purchased from Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals and solvents were of reagent grade.