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P22077

Manufactured by MedChemExpress
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Sourced in United States

P22077 is a chemical compound produced by MedChemExpress. It is a lab reagent used for research purposes. The core function of P22077 is to serve as a tool for scientific investigations, but its specific applications are not provided in this description.

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Lab products found in correlation

Hbx19818, by MedChemExpress (2 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Siusp7, by GenePharma (1 mentions) Crenolanib, by Selleck Chemicals (1 mentions) Midostaurin, by Selleck Chemicals (1 mentions)

4 protocols using p22077

1

Protein stability analysis via USP7 inhibition

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HEK293T cells were transfected with siUSP7 (GenePharma. Shanghai, China) or were treated with USP7 inhibitor, P22077 (Cat #HY-13865, MedChemExpress, NJ, USA). After 24 h of incubation, Cycloheximide (CHX) (100 μg/ml) (Cat #01810, Sigma-Aldrich, St. Louis, MO, USA) was treated into HEK293T cells. Samples were harvested in 6, 12, and 24 h later. And then, the protein stability was determined by Western blotting analysis.
Park H.B., Hwang S, & Baek K.H. (2022). USP7 regulates the ERK1/2 signaling pathway through deubiquitinating Raf-1 in lung adenocarcinoma. Cell Death & Disease, 13(8), 698.
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2

Analyzing DUB Inhibitor Compounds

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DUB inhibitors HBX19818 and P22077 were purchased from Medchem Express and dissolved in DMSO to obtain a 10 mM stock solution. HBX19818 analogs were purchased from ChemDiv and dissolved in DMSO to obtain a 10 mM stock solution. UPLC-MS analysis of all compounds was consistent with reported purity and molecular weight. Serial dilutions were then made, to obtain final dilutions for cellular assays with a final concentration of DMSO not exceeding 0.1%.
Weisberg E.L., Schauer N.J., Yang J., Lamberto I., Doherty L., Bhatt S., Nonami A., Meng C., Letai A., Wright R., Tiv H., Gokhale P.C., Ritorto M.S., De Cesare V., Trost M., Christodoulou A., Christie A., Weinstock D.M., Adamia S., Stone R., Chauhan D., Anderson K.C., Seo H.S., Dhe-Paganon S., Sattler M., Gray N.S., Griffin J.D, & Buhrlage S.J. (2017). Inhibition of USP10 induces degradation of oncogenic FLT3. Nature chemical biology, 13(12), 1207-1215.
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3

DUB Inhibitor Compound Preparation

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Midostaurin and crenolanib were purchased from Selleckchem (Houston, TX). DUB inhibitors HBX19818 and P22077 were purchased from Medchem Express. HBX19818 analogues, including C673-0105, C598-0556, C598-0563, C598-0466, C598-0515 and C598-0468, were purchased from ChemDiv. UPLC-MS analysis of all compounds was consistent with reported purity and molecular weight. All inhibitors were dissolved in DMSO to obtain a 10 mM stock solution. Serial dilutions were then made, to obtain final dilutions for cellular assays with a final concentration of DMSO between 0.2 and 0.5%.
Yang J., Meng C., Weisberg E., Case A., Lamberto I., Magin R.S., Adamia S., Wang J., Gray N., Liu S., Stone R., Sattler M., Buhrlage S, & Griffin J.D. (2020). Inhibition of the deubiquitinase USP10 induces degradation of SYK. British Journal of Cancer, 122(8), 1175-1184.
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4

BH4, Bortezomib, and P22077 Treatment

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BH4 was purchased from Sigma. BH4 was dissolved in RPMI 1640 medium or PBS to a stock concentration of 20 mM. Bor was purchased from Selleckchem. P22077 (USP inhibitor, USPi) was purchased from MedChemExpress. Bor and P22077 were dissolved in dimethylsulfoxide (DMSO) to stock concentrations of 80 mM and 150 mM. For cell assays, BH4 was used at a concentration of 40 μM, Bor at 15 nM, and P22077 at 10 μM. For in vivo use, BH4, Bor, and P22077 were injected intraperitoneally (i.p.) at doses of 60 μg/mouse, 20 μg/mouse, and 200 μg/mouse.
Zhang H., Chen J., Zhang M., Zhao M., Zhang L., Liu B, & Wang S. (2022). Tetrahydrobiopterin induces proteasome inhibitor resistance and tumor progression in multiple myeloma. Medical Oncology (Northwood, London, England), 39(5), 55.
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