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Sutter p 2000 laser puller

Manufactured by Sutter Instruments
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Sourced in United States

The Sutter P-2000 is a laser-based micropipette puller. It is designed to create precise and consistent micropipettes from glass or quartz capillaries. The device uses a CO2 laser heat source to heat and pull the capillary, allowing for the creation of micropipettes with customizable tip diameters and taper lengths.

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Lab products found in correlation

Loctite 401, by Henkel Adhesive Technologies (1 mentions) Supra 55 fesem, by Zeiss (1 mentions) Ltq orbitrap xl mass spectrometer, by Thermo Fisher Scientific (1 mentions) Fused silica capillaries, by Molex (1 mentions)

Spelling variants of this product

P-2000 (105 citations) P-2000 laser puller (29 citations) P-2000 puller (10 citations) Laser puller (9 citations) Sutter P-2000 (7 citations) Sutter P-2000 laser micropipette puller (2 citations)

3 protocols using sutter p 2000 laser puller

1

Microinjection of Drosophila Embryos

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Eggs were injected with a short taper quartz glass capillary with filament (inside diameter 1.0 mm and outside diameter 1.0 mm; Sutter Instruments) pulled with a Sutter P-2000 laser puller (1 line: heat = 700; filament = 2; velocity = 30; delay = 130; pull = 75) (Sutter Instruments). The underside of the glass slide with eggs was adhered with water to the glass stage plate on a Zeiss AxioZoom V16 stereomicroscope fitted with an aureka digital micromanipulator (Aura Optik) set at a 45° angle. A Narishige IM-300 microinjector (Narishige) with nitrogen-sourced pressure standing at 62.0 psi and an initial pressure of 20.0 psi was used during initial injection with further adjustments, as needed, down to 2.0 psi. The pressure was lowered to adjust to the fine capillary tip breaking off from repeated injection but maintains a thin enough taper to keep injecting with high survivability. After injection, wounds were sealed with cyanoacrylate adhesive (Loctite 401; Henkel) and the slide was placed in a fly vile with a wet cotton bottom and a sponge stopper; eggs were then placed in a climate chamber.
Fandino R.A., Haverkamp A., Bisch-Knaden S., Zhang J., Bucks S., Nguyen T.A., Schröder K., Werckenthin A., Rybak J., Stengl M., Knaden M., Hansson B.S, & Große-Wilde E. (2019). Mutagenesis of odorant coreceptor Orco fully disrupts foraging but not oviposition behaviors in the hawkmoth Manduca sexta. Proceedings of the National Academy of Sciences of the United States of America, 116(31), 15677-15685.
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2

Fabrication of SECCM Pipets

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Pipets used for SECCM were pulled from borosilicate theta capillaries (TG C150-10, Harvard Part No. 30-0114) using a Sutter P-2000 laser puller (Sutter Instruments, USA). The total inner diameters of pipets were in the range 600 nm–1 μm, determined accurately by scanning electron microscopy (SEM) using a Zeiss SUPRA 55FE-SEM. The outer walls of the pipets were rendered hydrophobic by silanization with dichlorodimethylsilane (99 + % purity, Acros), by flowing argon through the pipet to protect the inside from silanization.
Liu D.Q., Kang M., Perry D., Chen C.H., West G., Xia X., Chaudhuri S., Laker Z.P., Wilson N.R., Meloni G.N., Melander M.M., Maurer R.J, & Unwin P.R. (2021). Adiabatic versus non-adiabatic electron transfer at 2D electrode materials. Nature Communications, 12, 7110.
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3

Single-Probe Device for Mass Spectrometry

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The Single-probe (Figure 2a and b) fabrication was described in detail in previous publications [33 (link), 34 (link)]. Briefly, the device was made from a dual-bore quartz tubing (outer diameter (o.d.) 500 µm; inner diameter (i.d.) 127 µm; Friedrich and Dimmock, Inc., Millville, NJ, USA) pulled to a fine needle using a Sutter P-2000 laser puller (Sutter Instrument, Novato, CA, USA). Two fused silica capillaries (o.d. 105 µm, i.d. 40 µm; Polymicro Technologies, Phoenix, AZ, USA) were placed inside each channel within the pulled dual-bore quartz needle at the flat end, sealed with UV activated bonding resin (Light Cure Bonding Adhesive; Prime-Dent, Chicago, Il, USA), and the whole device was then secured onto a glass slide using regular epoxy. One of the fused silica capillaries acts as the solvent providing inlet whereas the other plays the role as the nano-ESI emitter (Figure 2b). The Single-probe device has been coupled with a XYZ translation stage system for sample motion control and a Thermo LTQ Orbitrap XL mass spectrometer for MS analysis (Figure 2a and c).
Rao W., Pan N., Tian X, & Yang Z. (2016). High-Resolution Ambient MS Imaging of Negative Ions in Positive Ion Mode: Using Dicationic Reagents with the Single-Probe. Journal of the American Society for Mass Spectrometry, 27(1), 124-134.
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