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Anti ly6g percp pe

Manufactured by Thermo Fisher Scientific
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The Anti-Ly6G-Percp-PE is a laboratory reagent that is used to detect and quantify Ly6G-positive cells, such as neutrophils, in biological samples. It is a fluorescent-labeled antibody that binds specifically to the Ly6G antigen, which is expressed on the surface of certain types of immune cells. The Percp-PE fluorophore allows for the detection and quantification of the labeled cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Anti cd11β fitc, by Thermo Fisher Scientific (2 mentions) Anti f4 80 percp, by Thermo Fisher Scientific (2 mentions) Anti cd45 apc, by Thermo Fisher Scientific (2 mentions) Facscalibur, by BD (2 mentions)

Close spelling variants of this product

Anti-Ly6G-PerCP Anti-Ly6G-PE Ly6G-PE Anti-Ly6G

2 protocols using anti ly6g percp pe

1

Microglial Cell Isolation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolated microglial cells from brain tissue and BV2 cells were washed in PBS twice. The cells were blocked with CD16/32 for 10 min and then stained for 30 min in a dark chamber with appropriate fluorochrome-conjugated antibodies (Anti-CD45-APC, eBioscience, Anti-CD11β-FITC, eBioscience, Anti-F4/80-Percp, eBioscience, Anti-Ly6G-Percp-PE, eBioscience). After washing thrice in PBS, flow cytometry was performed to analyze microglial cell populations using a BD FACSCalibur flow cytometer (BD Bioscience, San Jose, CA) and FlowJo software (Tree Star Inc., Ashland, OR).
Xu F., Mu J., Teng Y., Zhang X., Sundaram K., Sriwastva M.K., Kumar A., Lei C., Zhang L., Liu Q.M., Yan J., McClain C.J., Merchant M.L, & Zhang H.G. (2021). Restoring Oat Nanoparticles Mediated Brain Memory Function of Mice Fed Alcohol by Sorting Inflammatory Dectin-1 Complex Into Microglial Exosomes. Small (Weinheim an der Bergstrasse, Germany), 18(6), e2105385.
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2

Microglial Cell Isolation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolated microglial cells from brain tissue and BV2 cells were washed in PBS twice. The cells were blocked with CD16/32 for 10 min and then stained for 30 min in a dark chamber with appropriate fluorochrome-conjugated antibodies (Anti-CD45-APC, eBioscience, Anti-CD11β-FITC, eBioscience, Anti-F4/80-Percp, eBioscience, Anti-Ly6G-Percp-PE, eBioscience). After washing thrice in PBS, flow cytometry was performed to analyze microglial cell populations using a BD FACSCalibur flow cytometer (BD Bioscience, San Jose, CA) and FlowJo software (Tree Star Inc., Ashland, OR).
Xu F., Mu J., Teng Y., Zhang X., Sundaram K., Sriwastva M.K., Kumar A., Lei C., Zhang L., Liu Q.M., Yan J., McClain C.J., Merchant M.L, & Zhang H.G. (2021). Restoring Oat Nanoparticles Mediated Brain Memory Function of Mice Fed Alcohol by Sorting Inflammatory Dectin-1 Complex Into Microglial Exosomes. Small (Weinheim an der Bergstrasse, Germany), 18(6), e2105385.
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