VEGF-B short hairpin RNAs (shRNA/sh) were synthesized by Vigene Biosciences. AhR small interfering RNA (siRNA/si) and the overexpression AhR (OE-AhR) plasmid (NM_001621.5) were provided by Guangzhou RiboBio Co., Ltd. The sequence of VEGF-B shRNA and AhR siRNA are shown in Additional file 1 : Table S1. In order to clone the VEGF-B (NM_003377) promoter region, gene-specific primers were designed to amplify a 2.0-kb (-2,000- + 50) genomic region upstream of the VEGF-B gene. For the generation of the luciferase reporter construct, the 2.0-kb VEGF-B promoter fragment was ligated into the pGL3-basic vector (RiboBio Co., Ltd). This plasmid was named VEGFB-wild-type (WT)1. A vector including a 1.0-kb (− 1000 to + 50) genomic region upstream of the VEGF-B gene was named VEGFB-WT2. The mutant type (VEGF-B Mut) was also cloned into the pGL3-basic vector.
Pgl3 basic vector
Manufactured by RiboBio
The PGL3-basic vector is a plasmid designed for basic gene expression studies. It contains a multiple cloning site for inserting genes of interest and a reporter gene for monitoring expression. The vector is widely used in molecular biology research for various applications.
Automatically generated - may contain errors
2 protocols using pgl3 basic vector
1
VEGF-B and AhR Regulation in Cells
2
NFIL3 Promoter Regulation by STAT3
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The NFIL3 promoter including the wild STAT3‐NFIL3 binding sites or mutant type was cloned into the pGL3‐Basic vector (Ribobio, Guangzhou, China). The luciferase reporter assay (Promega, Madison, WI) executed following the luciferase reporter assay protocol. JEG‐3 cells were co‐transfected with reporter plasmid (pGL3‐NFIL3 wt/mt), STAT3, shSTAT3, or treated with 50 ng/ml IL‐6 (Sigma) treatment as indicated. The fluorescence value was detected by GloMax‐multimode reader (Promega).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!