Anti cd31 pe

Detailed methods are provided in the online version of this paper and include the following: Resource dissociated by gentle trituration using a 1 mL pipette. Dissociated cells were layered on the top of 5 mL of Ovomucoid inhibitor solution (Worthington Biochemical Corp.) and harvested by centrifugation (140 x g for 6 min). This method routinely yielded $2 3 10 6 cells per mouse brain. Cell aggregates were removed by filtering with 30 mm cell strainers (Becton Dickinson). The cell suspension was then labeled for oligodendrocyte marker 1:50 anti-O4-PE (Miltenyi Biotec), endhothelial cell marker 1:50 anti-CD31-PE (Miltenyi Biotec) and microglial marker 1:50 anti-CD11b-APC (Miltenyi Biotec), according to the standard manufacturer's protocol. To collect astrocytes, GFAP/EGFP mice were used. The cells were kept on ice until sorting.